Reagent case for extracting zooxanthellae genom from ledge rock coral and method thereof

Abstract

The invention provides a reagent kit which extracts zooxanthella genome DNA from reef building coral and a method of the reagent kit. The reagent kit comprises: reagent A (lysis buffer), reagent B (protease E), reagent C (RNAase A), reagent D (supersaturation NaC1 solution), reagent E (DNA cleaning mixture) and reagent F (DNA eluent). The method of the invention comprises: firstly, using the lysis buffer which contains SDS to crack tissue cells, then, providing a high salt low PH circumstance, enabling protein in solution to degenerate further, but combining DNA on a silica gel column, and finally, quantitatively reclaiming the DNA from a chromatography column in water-soluble buffer solution. The zooxanthella genome DNA can be rapidly and conveniently extracted from varous reef building coral, the DNA which is obtained can be used to research molecular ecology, systematic evolution and the like, and thereby the demands of regular monitoring tasks in coral reef nature reserves can be satisfied.

Description

technical field [0001] The invention relates to a kit for extracting zooxanthellae genome DNA from reef-building stony corals and a method for extracting reef-building stony coral symbiotic zooxanthellae genome DNA using the kit. Background technique [0002] Coral reefs have extremely high biodiversity. 25% of the world's marine life lives in coral reefs. They are known as "Oasis of the Sea" or "Marine Tropical Rainforest", and their socio-economic value is immeasurable. The coral reef ecosystem has extremely rich biological resources and is a breeding base for fisheries, providing human beings with important food and medicine resources; the coral reefs on the seabed have beautiful scenery and are famous underwater gardens. They are an excellent diving tourist destination for people. Therefore, the protection and sustainable use of coral reefs has attracted people's attention. For example, my country has established the Sanya Coral Reef National Nature Reserve in Hainan and...

AI Technical Summary

Problems solved by technology

Because zooxanthellae live symbiotically in reef-building corals, and reef-building corals themselves have calcium carbonate skeletons, calcium carbonate salts will mechanically shear genomic DNA fragments during the extraction process, thereby increasing the extraction of high-quality zooxanthellae genomic DNA. Difficulty

However, the conventional phenol / chloroform extraction method is not suitable for routine detection due to the cumbersome method and the toxicity of organic solvents.