Polysaccharide produced by microorganism belonging to genus bifidobacterium

一种微生物、多糖的技术，应用在细菌、药物组合、食物科学等方向，能够解决功能未明确等问题

Inactive Publication Date: 2008-07-09
MORISHITA JINTAN CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, regarding polysaccharides produced by these Bifidobacterium genus microorganisms, only glucose-containing polysaccharides produced by Bifidobacterium longum (Japanese Patent Application Laid-Open No. 7-255465), Bifidobacterium longum, and Bifidobacterium longum have been reported so far. Produced polysaccharides containing glucose, galactose, uronic acid, etc. (Appl. Microbiol. Biotechnil. Vol. 43, pp. 995-1000), etc., and their functions have not been clarified

Method used

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  • Polysaccharide produced by microorganism belonging to genus bifidobacterium
  • Polysaccharide produced by microorganism belonging to genus bifidobacterium
  • Polysaccharide produced by microorganism belonging to genus bifidobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] (Example 1: isolation of microorganisms)

[0095] (preparation of medium)

[0096] As a medium for isolating bifidobacteria, BL agar medium (Nissui Pharmaceutical Co., Ltd.) and BS agar medium were used. The BL agar medium was sterilized in an autoclave at 115° C. for 20 minutes, added 5% horse blood under aseptic conditions, and dispensed into petri dishes for use as plates. BS agar medium was prepared as follows. First, 30 g of sodium propionate, 100 mg of paromomycin sulfate, 400 mg of neomycin sulfate, and 6 g of lithium chloride were dissolved in purified water to make the total amount 100 ml, and an additive solution for BS agar medium was prepared. 50 ml of this supplemented BS agar medium was added to 1000 ml of BL agar medium to obtain a BS agar medium. This BS agar medium was dispensed onto a petri dish and used as a plate.

[0097] (Screening of microorganisms)

[0098] Human feces were dissolved in sterile water, inoculated on the above-mentioned BL aga...

Embodiment 2

[0099] (Example 2: Structural Analysis of Polysaccharides)

[0100] (preparation of medium)

[0101] Pancreatin preparation (Amano Enzyme Co., Ltd.) and silicon were added to a 9% by mass skim milk solution so that the final concentrations were 0.36% by mass and 0.01% by mass, respectively. Next, the pH of the solution was adjusted to 8 with 10N NaOH, and reacted at 55° C. for 4 hours to obtain enzymatic skim milk. Cultivator (Yaizu Suisan Chemical Industry Co., Ltd.), lactose, and sodium ascorbate were added thereto so that the final concentrations were 3.0% by mass, 2.5% by mass, and 0.2% by mass, respectively, and sterilized with an autoclave at 121° C. for 15 minutes. Used as a liquid medium.

[0102] (purification of polysaccharides)

[0103] The Bifidobacterium longum JBL05 strain (NITE BP-82) pre-cultivated with the liquid medium prepared above was inoculated into the same liquid medium at a concentration of 1% (v / v), and carried out at 37°C for 40 hours. Static ana...

Embodiment 3

[0118] (Example 3: cultivation and production of polysaccharides)

[0119] Sodium ascorbate was added to the enzymatically digested skim milk prepared in Example 2 so that the final concentration was 0.2% by mass, and it was sterilized with an autoclave at 121° C. for 15 minutes to prepare a liquid medium. The culture solution of Bifidobacterium longum JBL05 strain (NITE BP-82) that is pre-cultivated with the same liquid medium is inoculated in the liquid medium prepared above, and the concentration is 1% (v / v), stirred at a low speed, The pH was controlled at 6.0, and anaerobic culture was carried out at 37°C for 40 hours. The pH was controlled using 10N NaOH. The case where static anaerobic culture was performed was set as a comparative example.

[0120] Fig. 1 shows the relationship between the culture time and the viscosity of the culture solution. Compared with the case of static anaerobic culture, when the pH was adjusted and the culture was performed, the viscosity i...

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Abstract

A polysaccharide comprises galactose, glucose, rhamnose and pyruvic acid as constituents, the galactose, glucose and rhamnose being contained at a molar ratio of 4:2:1 and the pyruvic acid being contained in an amount of 4 to 7% by mass. The polysaccharide can be produced by culturing cells of Bifidobacterium longum strain JBL05 (NITE BP-82) under anaerobic conditions.

Description

technical field [0001] The present invention relates to novel polysaccharides produced by microorganisms belonging to the genus Bifidobacterium and compositions of food, cosmetics, and medicines containing the polysaccharides. Background technique [0002] Microorganisms of the genus Bifidobacterium are one of the most dominant flora in the intestinal flora. Like lactic acid bacteria, this microorganism can directly ingest the bacterial cells themselves. It has been reported that ingestion of these microorganisms provides an intestinal regulation effect of adjusting the balance of intestinal flora, an effect of improving serum cholesterol levels, an immune activation effect, and the like. Among them, for the enhancement of immune function, an antitumor agent (Japanese Patent Application Laid-Open No. 7-82158 ) and a cytokine production promoter (Japanese Patent Laid-Open No. Gazette), a drug for the prevention and treatment of inflammatory bowel disease using microorganism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/30A61K8/00A61K8/72A61K31/715A61P37/04A61Q19/00C08B37/00C12P19/04
CPCC08B37/0036C12P19/04A61Q19/00A23L1/3014A61K8/73A61K8/99A23L33/135A61P37/04C08B37/00C12N1/20
Inventor 浅田雅宣河野麻实子金谷忠吉野智惠松浦洋一河原有三北村进一
Owner MORISHITA JINTAN CO LTD
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