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Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum

An electrospray ion source and separation system technology, applied in the field of ion sources, can solve the problems of different ionization efficiency, inability to realize the combination of multiple liquid phase separation systems, and increase ionization efficiency, etc., and achieves strong versatility and practicability. , the structure is simple, the effect of increasing the ionization efficiency

Inactive Publication Date: 2012-04-11
徐州脉析医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the inconsistent distance between each spray port and the mass spectrometer inlet, the ions and ionization efficiency generated by each port are different. Therefore, this interface can only be used to increase the ionization efficiency of the sample, and cannot realize multiple sets of liquid phase separation systems. Combined use of mass spectrometry

Method used

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  • Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum
  • Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum
  • Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Such as figure 1Shown is a multi-channel electrospray ionization source coupled with a micro-liquid phase separation system and a mass spectrometer. A five-channel design is adopted, that is, the ion source 1 is composed of a heating capillary sleeve 2, a spray chamber 3, and five sockets 4 for installing a single-channel electrospray nozzle / nozzle distributed in a fan-shaped space. In addition, 5 is a nitrogen inlet, and 6 is a nitrogen outlet. Needles / nozzles suitable for different modes of separation systems coupled with mass spectrometers can be easily installed in 4, and the best spray effect can be achieved by adjusting the distance between the needles / nozzles and the inlet of the vacuum region of the mass spectrometer (heated capillary orifice). In this embodiment, ensure that the central axis of each single-channel electrospray nozzle / nozzle end intersects at the position of 0.8 cm from the front end of the vacuum zone inlet, so that the central axes of the fou...

Embodiment 2

[0035] Experimental conditions: capillary (Hebei Yongnian Ruifeng Chromatography Device Co., Ltd., inner diameter: 50 μm, outer diameter: 375 μm), syringe pump (Harvard, USA), LCQ duo Ion trap mass spectrometry (U.S. Finnigan company), the ion source used is the five-channel micro-liquid phase separation system described in embodiment 1 and the electrospray ion source coupled with mass spectrometry, the central axis of each single-channel electrospray nozzle / nozzle end Intersect at the position 0.6cm from the front end of the inlet of the vacuum area, so that the central axis of the end of the four single-channel electrospray nozzles / nozzles is at an angle of 45 degrees to the axis of the vacuum inlet, and the spray voltage is 2.2kV.

[0036] Sample: a polypeptide (m / z=815) (0.1% formic acid aqueous solution)

[0037] see image 3 , which is obtained by using a micro-liquid phase separation system provided in Example 1 of the present invention coupled with a mass spectrometer...

Embodiment 3

[0039] Experimental conditions: Michrom multidimensional liquid chromatography, LCQ duo Ion trap mass spectrometry (Finnigan, USA). The ion source used is the five-channel micro-liquid phase separation system described in Example 1 and the electrospray ion source coupled with mass spectrometry. The central axis of each single-channel electrospray nozzle / jet end intersects at the position of 1 cm from the front end of the vacuum zone inlet At the position, the central axes of the four single-channel electrospray needle / nozzle ends are at an angle of 45 degrees to the vacuum inlet axis, and the spray voltage is 3kV.

[0040] Liquid conditions: Chromatographic column: 250μm×50mm, 5μC18

[0041] Mobile phase: A: 2% ACN (containing 0.1% formic acid, 0.05% HFBA) B: 98% ACN (containing 0.1% formic acid, 0.05% HFBA); 60min gradient: 0min: 10%A; 50min: 40%; 55min: 60%A; 60min: 60%A; injection volume 2μL; flow rate 5μL / min.

[0042] Sample: 50μg / mL β-Casein hydrolyzate

[0043] see ...

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Abstract

The invention relates to a multi-channel electrospray ion source used in the combination of a micro liquid phase separation system and mass spectrum, wherein 2 to 100 single-channel electrospray nozzle needles / nozzles are fixed on the inlet of the vacuum area of a mass spectrometer, and are distributed in a sector, thereby ensuring that the central axial lines of the tail ends of the single-channel electrospray nozzle needles / nozzles are crossed at the position of 0cm to 1cm away from the front end of the inlet of the vacuum area and are at an angle of 0 degree to 90 degrees to the axial lineof the vacuum inlet. When high voltage is respectively applied on each electrospray nozzle needle / nozzle, electrospray ionization of multi-flow path liquid phase can be realized, thereby realizing the combination of a liquid phase separation system and mass spectrum. The ion source has the characteristics of simple structure, easy debugging operation, convenient mode switching, excellent stability and low maintenance cost, etc., and can be directly fixed on a mass spectrometer along with better commonality and practicability. In addition, through installing electrospray nozzle needles / nozzlesof different modes, the invention can realize the combination of micro liquid phase separation systems of a plurality of modes and mass spectrum.

Description

technical field [0001] The invention relates to an ion source used in conjunction with a liquid phase separation system and a mass spectrometer, in particular to a multi-channel electrospray ion source used in conjunction with a micro liquid phase separation system and a mass spectrometer. The combination of multiple sets of micro-liquid phase separation systems and mass spectrometry can be realized at the same time. Background technique [0002] In the past decade, electrospray mass spectrometry (ESI-MS) has developed into a general mass spectrometry technique, which has been applied to the analysis of many small molecule samples and biological samples, even for the analysis of non-volatile and thermally unstable compound. [0003] With the wide application of electrospray mass spectrometry, the coupling technology of various liquid phase separation systems and mass spectrometry has also begun to mature. Micro-separation technology is one of the emerging technologies deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/72H01J49/10
Inventor 张丽华段继诚梁作成袁辉明张维冰梁振王晖张玉奎
Owner 徐州脉析医疗技术有限公司
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