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Humanized cell factor hair agent and preparing method thereof

A cytokine and human-derived technology, applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of inability to decrease biological activity, easy degradation of drugs, and difficulty in reaching effective concentrations, etc. problems, to achieve the effect of reducing adverse reactions, increasing retention volume and retention time, and applying safe and non-irritating effects

Inactive Publication Date: 2008-06-18
何荫良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for such drugs to directly reach the hair follicle tissue through the skin barrier, and it is difficult to achieve an effective concentration in the local hair follicle; at the same time, these drugs cannot be treated through intravenous or oral administration due to their easy degradation and decreased biological activity.

Method used

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  • Humanized cell factor hair agent and preparing method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Extraction of Active Cytokines in Cell Culture Supernatant

[0021] Use the ammonium sulfate fractional precipitation method to concentrate and extract active cytokines: collect the cell culture supernatant, add ammonium sulfate until the ammonium sulfate saturation reaches 30%, wait for the dissolution to be completed, let it stand for 30 minutes, then centrifuge at 20°C, 3000r / min for 30 minutes, and separate Clear and precipitate. The precipitate was suspended with PBS, ammonium sulfate was added to the supernatant until the ammonium sulfate saturation reached 50%, and centrifuged again. Repeat once until the saturation of ammonium sulfate reaches 70%, collect the precipitate three times, use PBS as the dialysate and W3500 dialysis bag for 3 hours to desalt, replace the dialysate every 1 hour, and use a magnetic stirrer to accelerate the desalination, collect the liquid in the dialysis bag, Freeze-dry at -70°C for 24 hours to obtain protein freeze-dried po...

Embodiment 2

[0022] The preparation of embodiment 2 human-derived cytokine hair growth preparation liposome dosage form

[0023] Weigh 1 g of egg yolk lecithin and 0.5 g of cholesterol, and dissolve them with 67 ml of chloroform and 33 ml of methanol. The solvent was evaporated on a RE-52 rotary evaporator in a water bath at 48°C and 120 r / min until a yellow-green milky lipid film was formed. Weigh 0.75 g of the dry protein powder described in Example 1, dissolve it with 12.5 ml of PBS, add it to the lipid film, add 100 ml of ether, and mix well. After oscillating and emulsifying for 10 minutes on a KQ-400KDE high-power ultrasonic cleaner at 20°C and 160W, use a RE-52 rotary evaporator to remove the solvent in a water bath at 38°C and 120r / min until curd / tofu-like coagulation Gel formed, suspended with 17.5ml PBS. Let stand for 1h. On the CP-750 ultrasonic breaker, ice bath, 150W, intermittent ultrasonic emulsification 100 times (ultrasonic 2s, intermittent 9s), to obtain a milky white ...

Embodiment 3

[0024] Morphological identification of embodiment 3 human-derived cytokine hair growth preparation liposome dosage form

[0025] Adjust the ultrasonic frequency and time as needed to control the particle size and shape of liposomes, and use transmission electron microscopy to identify: 2% phosphotungstic acid staining, copper grid drying, observe liposome morphology under a transmission electron microscope after 15 minutes, take pictures and measure its diameter. The average diameter of the prepared liposomes is 140.8nm (59.3-296.3nm), and the morphology is shown in Figure 1. The results show that the liposomes prepared by this process are relatively uniform in size, and the membrane edges are smooth and wrinkle-free.

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Abstract

The invention discloses a human-derived cell-factor germinal preparation and the preparation method, relating to the technical filed of the healthcare product. The invention contains lecithin which is the lipid membrane-forming material, cholesterin and active component germinal cell factor, with the proportion by weight between them being 2-6:1-4:1-5; the lecithin is selected from egg yolk lecithin or soybean lecithin; the germinal cell factor is selected from the albumen freeze-dry powder of the culture supernatant of human fibroblasts, placental cells, cuticle cells or dermal papilla cells, with abundant cell factors for the hair growth in the culture supernatant; the germinal cell factors are enveloped in the lipid membrane. The rotary evaporating method is adopted in the process to make the product the lipidosome medicament form which can easily permeate the skin barrier. In addition, the product is frozen and dried into the more stable powder, which needs only to be dissolved by the distilled water containing 2% penetrating enhancer azone while in use. The invention is of easy penetration, high skin retention and good curative effect and free of side effect.

Description

technical field [0001] The invention relates to a hair growth preparation and a preparation method thereof, in particular to a human-derived cytokine hair growth preparation and a preparation method thereof, and also to a liposome formulation of the human-derived cytokine hair growth preparation. Background technique [0002] According to epidemiological investigation, the incidence of hair diseases in the world is very serious, and the total incidence rate reaches 60%, wherein the male incidence rate is 74.6%, women's 24.4%, and alopecia patients account for 77.3%. Hair follicles play a key role in hair growth and development. At present, the basic research on the growth and development of hair follicles and hair diseases has made remarkable progress, but the clinical drugs for hair diseases are still relatively scarce. FDA has only certified the internal drug finasteride and the external drug minoxidil for the treatment of hair loss. Class 2 drugs. There are many traditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61K9/19A61K47/24A61P17/14A61K35/12A61K35/36A61K35/50A61K35/33
Inventor 邓国宏伍津津何荫良谭文婷岳世韬高小燕
Owner 何荫良
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