Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzyme test peper for detecting hydrogen peroxide concentration

The technology of horseradish peroxidase and test paper, which is applied in the field of enzymatic analysis in the field of biotechnology, can solve the problems of complicated operation, unfavorable wide use and the like, and achieves the effect of being easy to carry and suitable for mobile operation.

Inactive Publication Date: 2009-07-22
SOUTH CHINA AGRI UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, another serious problem of hydrogen peroxide residues in food is that many criminals in the market abuse hydrogen peroxide to bleach watery foods such as beef louver, chicken feet and bamboo shoots, and even to bleach and eliminate the peculiar smell of spoiled seafood. Wait
However, these methods are cumbersome to operate and require special laboratory equipment such as spectrophotometers, which is not conducive to the widespread use in the food industry and other monitoring fields.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme test peper for detecting hydrogen peroxide concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0017] Take thick qualitative filter paper and cut it into 1cm×3cm size, soak it in 1% carboxymethyl cellulose aqueous solution for about 60 minutes, after fully imbibition, dry it, and then soak the filter paper strip in 20ml containing color reagent And in the mixed solution of horseradish peroxidase, wherein the composition of mixed solution is: phenol 0.025M, 4-aminoantipyridine 0.0075M, horseradish catalase 0.025g / L (W / V), ampicillin Penicillin 100mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

Embodiment 2

[0019] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0020] Cut the carboxymethyl cellulose membrane into a size of 1cm×3cm (because the carrier itself is carboxymethyl cellulose, which already has negatively charged anionic groups, so it is not necessary to soak it in carboxymethyl cellulose aqueous solution), then soak it in 20ml In the mixed solution of chromogen and horseradish peroxidase, wherein the mixed solution consists of: phenol 0.02M, 4-aminoantipyridine 0.005M, horseradish catalase 0.01g / L (W / V ), tetracycline 10mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

Embodiment 3

[0022] Preparation of Enzyme Test Paper for Determination of Hydrogen Peroxide Concentration

[0023] Cut the nitrocellulose membrane into a size of 1cm×3cm (because the carrier itself is nitrocellulose, which already has a negatively charged anion group, so it is not necessary to soak it in an aqueous carboxymethyl cellulose solution), and then soak it in 20ml of chromogen and In the mixed solution of horseradish peroxidase, wherein the mixed solution is composed of: phenol 0.10M, 4-aminoantipyridine 0.05M, horseradish catalase 0.025g / L (W / V), kana Mycin 50mg / L (W / V). After shaking at 4°C for 60 minutes, take out the filter paper, dry it at low temperature, and finally store it in a sealed plastic bag at low temperature and avoid light. That is, the enzyme test paper that can be used for the determination of hydrogen peroxide concentration is prepared.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an enzyme test paper which can rapidly detect the content of hydrogen peroxide in a sample prepared by enzymatic color reaction. The test paper is prepared through the method of immobilizing the positively charged horseradish peroxidase and the color developing agent on the carrier simultaneously by utilizing the adsorption effect of the anion carrier. The test paper can detect the presence of hydrogen peroxide in the sample at a concentration as low as 0.5 μmol / L. The response time of the test paper is 1 minute during detection. It can conveniently and quickly determine the content of hydrogen peroxide in the sample. At the same time, the test paper of the invention is easy to carry and is suitable for mobile operations.

Description

technical field [0001] The invention relates to an enzyme test paper for rapidly detecting the content of hydrogen peroxide in a sample by using an enzymatic color reaction, and belongs to the field of enzymatic analysis in the field of biotechnology. Background technique [0002] Hydrogen peroxide, commonly known as hydrogen peroxide, can be decomposed into water and oxygen under conditions such as stirring and light, and is extremely oxidizing and corrosive, so it is widely used as a disinfectant and bleaching agent for food. According to my country's "Hygienic Standards for the Use of Food Additives", food-grade hydrogen peroxide is allowed to be used as a processing aid in food production, but it should be removed before it is made into a finished product, and the residue should be strictly controlled. Excessive hydrogen peroxide residues in food will cause serious harm to the human body, such as: damage the digestive tract, cause cancer, accelerate human aging, induce c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78C12Q1/00
Inventor 王炜军吕志刘娥娥方颖徐凤彩
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com