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Viscosity reduction bacteria for increasing recovery ratio of petroleum, and application

A technology for application and oil recovery, applied in the direction of bacteria, drilling composition, and microbial-based methods, can solve problems such as poor hydrocarbons, unsatisfactory effects, and application constraints, so as to reduce fluidity and improve crude oil flow capacity , Improve the effect of the sweep coefficient

Inactive Publication Date: 2009-06-24
厦门绿启源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1955, La Riviere observed the surface tension of Pseudomonas aeruginosa, mycobacteria, and Bacillus subtilis in cultures such as glycerol. The surface tension decreased significantly, but the use of hydrocarbons was not good.
In 2002, Ni Yin used Corynebacterium, Mycobacterium and Nocardia as demulsifiers, but they must be combined with chemical demulsifiers and inorganic salts to reduce the oil-water interfacial tension, and the application is restricted
In 2004, Hou Zhaowei screened out that Bacillus cereus and Bacillus brevus have certain effects in improving the rheology of crude oil and reducing the interfacial tension between oil and water, but the effect is not ideal under high temperature conditions

Method used

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  • Viscosity reduction bacteria for increasing recovery ratio of petroleum, and application
  • Viscosity reduction bacteria for increasing recovery ratio of petroleum, and application
  • Viscosity reduction bacteria for increasing recovery ratio of petroleum, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, Streptococcus zooepidemicus (Streptococcus zooepidemicus) CGMCC NO.1988 to crude oil (oil-water ratio is 5-0.2, crude oil comes from Xinjiang Karamay Oilfield, Henan Zhongyuan Oilfield, Heilongjiang Daqing Oilfield) degradation

[0049] (1) Growth curve

[0050] Inoculate Streptococcus zooepidemicus CGMCC NO.1988 directly into 100ml of crude oil, culture it on a shaker at 20-60°C, 120-180r / min, and regularly measure the density (OD value) of bacteria in the crude oil. is the abscissa, and the OD value is the ordinate to draw its growth curve, and the results are shown in Figure 2. It can be seen from the growth curve that the bacteria enter the logarithmic phase at 4 hours of crude oil, and enter the stable phase at about 10 hours, which shows that the bacteria multiply very quickly in crude oil, which is beneficial to practical applications.

[0051] (2) Dispersion and emulsification effect

[0052] Take 5ml of Streptococcus zooepidemicus (Streptococcus...

Embodiment 2

[0083] Embodiment 2, the analysis of Streptococcus zooepidemicus CGMCC NO.1988 strain fermented liquid

[0084] (1) Qualitative and quantitative analysis of organic acids in fermentation broth

[0085] ① Qualitative analysis results of organic acids

[0086] Using Shimadzu GC-14C gas chromatography: FFAP quartz capillary column (0.250×30m), the carrier gas is high-purity nitrogen, the flow rate is 1ml / min, the temperature of the vaporization chamber is 220°C, the column temperature is programmed to rise, and the starting temperature is 90°C. The rate is 3°C / min, the final temperature is 200°C, and the injection volume is 1.0 μl. The organic acid is qualitatively determined according to the conventional method. The chromatogram of the organic acid standard sample is shown in Figure 6a, and the retention time under the chromatographic conditions is shown in Table 5. shown. The organic acid gas chromatograms of crude oil samples before and after the treatment of Streptococcus z...

Embodiment 3

[0104] Embodiment 3, subsequent treatment of the oil produced by Streptococcus zooepidemicus CGMCC NO.1988

[0105] Subsequent treatment of crude oil includes: dehydration and desalination.

[0106] (1) Dehydration

[0107] Water-containing crude oil must be dehydrated before being transported from the oil field to reduce transportation costs. To separate the crude oil from water, the crude oil needs to be demulsified. Demulsification is the process of transforming a relatively stable system into a relatively unstable system. At present, the commonly used demulsification methods include mechanical demulsification, electric demulsification and chemical demulsification.

[0108] Mechanical demulsification includes gravity sedimentation, centrifugal separation, fiber bed filtration, super filtration, etc. Due to its slow demulsification speed and high equipment investment, large-scale application of mechanical demulsification is limited; electric demulsification is performed un...

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Abstract

The invention discloses a slime reducing bacterium for enhancing oil recovery and application thereof. The Mycosis bacterium provided by the present invention is Streptococcus zooepidemicus (Streptococcus zooepidemicus) CGMCC NO.1988. Experiments have proved that Streptococcus zooepidemicus can quickly and effectively improve the properties of crude oil. There is no need to add other nutrients to the crude oil. It is directly connected with the bacterial liquid and fermented at 20-60°C for 8-72 hours, and the viscosity of the crude oil is changed from 8000- 15000MPa·s drops to 50-250MPa·s, a drop as high as 160 times; the acid value of crude oil increases by 8-15 times; light components increase, the wax and gum content decreases by 50-95%, the rheological properties of crude oil become better, oil-water The interfacial tension decreases, and the content of organic acids and other substances in the fermentation broth increases; moreover, Streptococcus zooepidemicus produces CO2 biogas and hyaluronic acid biopolysaccharides during the fermentation process, which makes the crude oil easier to emulsify, wet and disperse, increases the pressure of the oil layer, and reduces Crude oil viscosity, improve crude oil flow ability. Therefore, the bacterial strain can be widely used in the field of oil production engineering, especially in the field of microbial enhanced oil recovery and the field of oil pipeline transportation.

Description

technical field [0001] The invention relates to a viscosity-reducing microorganism for enhancing oil recovery and its application in the field of bioengineering, in particular to a viscosity-reducing bacterium for enhancing oil recovery and its application. Background technique [0002] Today's world is undergoing a new technological revolution marked by bioengineering and its application, and bioenergy technology has become one of the fields with the greatest economic potential, the most promising and promising prospects in bioengineering. These factors have promoted the development of microbial enhanced oil recovery technology. research and application. [0003] At present, in the petroleum industry, the primary oil recovery is a process in which crude oil is sprayed out of the ground through the oil well relying on the underground pressure. With the decrease of the underground pressure, the self-spraying ends, and about 10-15% of the underground crude oil can be recovered...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12S1/00C09K8/582C12R1/46
Inventor 张鹏张淑荣刘春巧
Owner 厦门绿启源生物科技有限公司
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