37results about How to "Realize cascade amplification" patented technology

The invention discloses a novel quick separation method of bacilluscereus, which provides the basis for the subsequent research for a target bacterium and relates to the technical field of biology. The novel quick separation method comprises the steps of: carrying out covalent coupling on a multi-arm star-shaped polymer and an antibody, coating the antibody-modified multi-arc star-shaped polymer on a long-chain biotin molecule, capturing the target bacterium in a sample solution by the antibody and long-chain biotin co-modified multi-arc star-shaped polymer, recognizing and coupling the long-chain biotin multi-arm star-shaped polymer in the sample solution by a streptavidin-modified nano magnetic bead, separating and heavily-suspending the captured bacterium; a heavily-suspended liquid can be directly analyzed in subsequent. Compared with a conventional bacterium magnetic separation method, the novel quick separation method is more suitable for carrying out magnetic separation on the bacterium in a complex matrix; the separation efficiency of the target bacterium in a sample is increased.

The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.

The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.

The invention discloses a method for enriching and separating human peripheral blood CD34<+> and CD91<+> stem cells, which provides basis for the subsequent researches on CD34<+> and CD91<+> stem cells better, and relates to the field of biomedicine. The method comprises the following steps of: covalently coupling a multi-arm well star-shaped polymer with a mouse anti-human CD34<+> or CD91<+> monoclonal antibody; coating long-chain biotin molecules on the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; capturing the CD34<+> and CD91<+> stem cells in a peripheral blood sample by the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; identifying and coupling the long-chain biotinylated well star-shaped polymer in the peripheral blood by nanometre magnetic beads modified by streptavidin; separating and re-suspending the captured CD34<+> and CD91<+> stem cells, and the like, wherein the re-suspension can be directly used for subsequent analysis. Compared with the traditional cell separation method, the method is more suitable for performing magnetic separation on the CD34<+> and CD91<+> stem cells in the complex peripheral blood sample, and capable of increasing the separation efficiency for the CD34<+> and CD91<+> stem cells in the peripheral blood sample.

The invention discloses a method for separating and enriching dendritic cells (Dendritice cells, DC) in whole blood for further providing a basis for subsequent study of dendritic cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of hyperbranched polymers and mouse anti-human dendritic cell monoclonal antibodies; further coating long-chain biotin molecules by hyperbranched polymers modified by mouse anti-human dendritic cell monoclonal antibodies; capturing DC in a whole blood sample by hyperbranched polymers co-modified by mouse anti-human dendritic cell monoclonal antibodies and long-chain biotin; identifying and coupling long-chain biotin hyperbranched polymers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured DC. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of DC in complex whole blood samples, so that the magnetic separation time is shortened, and the DC separation efficiency in the whole blood sample is improved.

The invention relates to the technical field of biology and discloses a method for enriching and separating yersinia enterocolitica (YE). The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacteria in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.

The invention discloses a high-efficiency rapid enrichment and separation method, mainly relates to a method for enriching and separating vibrio parahemolyticus from a matrix, aims at providing basis for follow-up study on target bacteria, and relates to the field of biotechnology. The method comprises the following steps of: carrying out covalent coupling on an arborescence hyper-branched polymer and an antibody, enveloping the antibody-modified arborescence hyper-branched polymer with long-chain biotin molecules, capturing the target bacteria in a sample liquid by using the arborescence hyper-branched polymer which is co-modified by the antibody and the long-chain biotin, recognizing and coupling the long-chain biotin arborescence hyper-branched polymer in the sample liquid by using streptavidin-modified nano magnetic beads, separating and heavy-suspending the captured bacteria, and the like. The captured bacteria can be subjected to follow-up analysis directly; and compared with the conventional bacterium separation method, the method disclosed by the invention is more applicable to magnetic separation of bacteria in a complex matrix, and the target bacterium separation efficiency in the sample is improved.

The invention discloses a method for separating and enriching natural killer cells (natural killer cells, NK) in peripheral blood for further providing a basis for subsequent study of natural killer cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of dendritic polymers and mouse anti-human NKp46 antibodies; further coating long-chain biotin molecules by dendritic polymers modified by mouse anti-human NKp46 antibodies; capturing NK in a peripheral blood sample by dendritic polymers co-modified by mouse anti-human NKp46 antibodies and long-chain biotin; identifying and coupling long-chain dendritic polymers in the peripheral blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured NK. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of NK in complex peripheral blood samples, so that the magnetic separation time is shortened, and the NK separation efficiency in the peripheral blood sample is improved.

The invention discloses a method for enriching and separating streptococcus pneumoniae in order to provide a basis for the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the steps of: covalent coupling between a dendritic polymer and an antibody, exposed amino of long-chain biotin blocking antibody-dendritic polymer, capturing of target bacteria in sample liquid by the antibody coupled with the dendritic polymer, identification of streptavidin coupled with nano magnetic beads and coupling with the long-chain biotinylated dendritic polymer in the sample liquid, magnetic separation and re-suspension of bacteria and the like. The re-suspension liquid can be directly subjected to follow-up analysis; and compared with the traditional magnetic bacterial separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicated substrate, and improves the separation efficiency of the target bacteria in a sample.

The invention discloses a method for directly separating CD<4+> and CD<8+> lymphocytes, lays a better foundation for the subsequent research on the CD<4+> and CD<8+> lymphocytes, and relates to the field of biomedicines. The method comprises steps of: multi-arm well and star-shaped polymer and mouse anti-human CD<4+> or CD<8+> monoclonal antibody covalent coupling, long-chain biotin molecule coating through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody-modified multi-arm well and star-shaped polymer, peripheral blood sample CD<4+> and CD<8+> lymphocyte acquiring through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody and long-chain biotin co-modified multi-arm well and star-shaped polymer, peripheral blood long-chain biotinylation multi-arm well and star-shaped polymer identifying and coupling through utilizing streptavidin-modified nano magnetic beads, captured CD<4+> and CD<8+> lymphocyte separating and suspending and the like. A suspension can be directly used for subsequent analysis; and compared with a conventional cell separating method, the method is suitable for magnetically separating complicated peripheral blood sample CD<4+> and CD<8+> lymphocytes, so that the peripheral blood sample CD<4+> and CD<8+> lymphocyte separation efficiency is increased.

The invention discloses a magnetic enrichment and separation method of Shigellaspp in a complex matrix. The method provides the basis for the follow-up study on target bacteria and relates to the technical field of biology. The method comprises the following steps of: covalently coupling hyperbranched polymers with antibodies, enveloping long-chain biotin molecules by the hyperbranched polymers modified with the antibodies, capturing target bacteria in a sample solution by the hyperbranched polymers modified with the antibodies and long-chain biotin, identifying and coupling long-chain biotinylated hyperbranched polymers in the sample solution by nano magnetic beads modified with streptavidin, and carrying out separation and heavy suspension on the captured bacteria. The captured target bacteria can be directly used for the follow-up analysis. Compared with the traditional magnetic separation method of bacteria, the method is suitable for magnetic separation of the bacteria in the complex matrix, and the separation efficiency of the target bacteria in a sample is improved.

The invention discloses a method for enrichment and separation, and mainly discloses a method for enriching and separating Candida albicans. The method provides a basis for better follow-up research on a target fungus, and relates to the technical field of biology. The method comprises the following steps that: a multi-arm star polymer is covalently coupled with an antibody, an antibody-modified multi-arm star polymer further coats long-chain biotin molecules, the multi-arm star polymer jointly modified by the antibody and the long-chain biotin molecules captures target bacteria in sample liquid, streptavidin-modified nano magnetic beads identify and couple with a long-chain biotinylated multi-arm star polymer in the sample liquid, the captured bacteria is separated and suspended again, and the like. The separated target bacteria can be directly subjected to follow-up analysis, and in comparison with a conventional bacteria separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the bacteria in a complex ground substance, and the separation efficiency of the target bacteria in the sample is improved.

The invention discloses a method for separating and enriching dendritic cells (Dendritice cells, DC) in whole blood for further providing a basis for subsequent study of dendritic cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of hyperbranched polymers and mouse anti-human dendritic cell monoclonal antibodies; further coating long-chain biotin molecules by hyperbranched polymers modified by mouse anti-human dendritic cell monoclonal antibodies; capturing DC in a whole blood sample by hyperbranched polymers co-modified by mouse anti-human dendritic cell monoclonal antibodies and long-chain biotin; identifying and coupling long-chain biotin hyperbranched polymers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured DC. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of DC in complex whole blood samples, so that the magnetic separation time is shortened, and the DC separation efficiency in the whole blood sample is improved.

The invention discloses a method of enrichment separation of meticillin-resistant Sta-phylococcusaureus (MRSA), provides a better basic for follow-up study of target bacteria, and relates to the field of biology technology. The method comprises steps of: covalently coupling broom molecules and antibodies, coating antibody-modified broom molecules with long-chain biotin molecules, capturing target bacteria in a sample solution via broom molecules that are modified by the antibodies and the long-chain biotin molecules, identifying streptavidin-modified nano-magnetic-beads and coupling with long-chain biotinylated broom molecules in the sample solution, separating and re-suspending the captured bacteria. Because the re-suspending solution can be used for follow-up analyses directly, the method provide by the invention is even more suitable for magnetic separation of bacteria in complex matrixes compared with conventional magnetic separation of bacteria, and rises the separating efficiency of target bacteria in samples.

The invention discloses a method for enrichment separation of mycobacterium tuberculosis (MT), and better provides a basis to the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the following steps of: performing covalent coupling of a hyperbranched polymer and an antibody; coating a long-chain biotin molecule with the antibody-modified hyperbranched polymer; capturing target bacteria in sample liquid by use of the hyperbranched polymer co-modified by the antibody and the long-chain biotin; identifying streptavidin-modified nano magnetic beads, and coupling with the long-chain biotinylation hyperbranched polymer in the sample liquid; separating and re-suspending the captured bacteria, and the like, wherein the re-suspension liquid can be directly subjected to follow-up analysis. Compared with a traditional magnetic bacterium separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicate matrix, and improves the separation efficiency of target bacteria in a sample.

The invention discloses a method for enriching and separating staphylococcus aureus (SA), provides a good basis to the subsequent research on a target bacterium, and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacterium in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.

The invention discloses a method for enriching and separating listeria monocytogenes (Listeria monocytogenes, Lm), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody, coating a long-chain biotin molecule on the antibody-modified dendrimer, capturing the target bacteria in a sample solution through the dendrimer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendrimer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.

The invention discloses a method for enriching and separating helicobacter pylori (Helicobacter pylori, Hp), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a hyperbranched polymer and an antibody, coating a long-chain biotin molecule on the antibody-modified hyperbranched polymer, capturing the target bacteria in a sample solution through the dendriform hyperbranched polymer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendriform hyperbranched polymer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.