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104results about How to "Avoid subjective errors" patented technology

Method for determining step-by-step speed limit value of exit ramp of freeway

The invention relates to a method for determining a step-by-step speed limit value of an exit ramp of a freeway, belonging to the field of traffic operation management of the freeway. The method comprises the following steps: dividing a speed characteristic value of the exit ramp into three site vehicle speeds, including a ramp nose end part, a transition section starting point and a reasonable outer side mainline of an exit ramp upstream section, through a research on running vehicle speed and deceleration of the exit ramp upstream section; deducing a running speed of the transition section starting point according to a ramp nose end speed limit value, continuously deducing a vehicle speed of the reasonable outer side mainline lane, combining an investigated or predicted mainline vehicle running speed to acquire a speed limit value calculation model and a corresponding calculation formula, finally determining a final step-by-step speed limit value according to engineering experience. Based on verification results of the method disclosed by the invention in combination with examples, the method disclosed by the invention has the advantages of simple and convenient calculation, high precision, suitability for engineering practical application and the like, effectively solves the shortcomings of the prior art and can be widely applied to the traffic operation management of the freeway.
Owner:SOUTHEAST UNIV

Electric transmission line house distribution graph generating method based on GIS mode

The invention relates to a house distribution graph generating method applied to a power system, in particular to an electric transmission line house distribution graph generating method based on a GIS mode. The method includes the specific steps of recording and managing properties of a house, automatically numbering the house, renaming batches of house photos, manually grouping of the house, outputting batches of distribution graphs and automatic loading and typesetting of correlated photos. The mode of outputting a house distribution graph in a flat profile graph in the prior art is changed and a GIS data processing method is adopted for directly editing and managing house graphs and property data in a plane graph. The house distribution graphs are output in batches according to a line path and the photos are automatically loaded. The electric transmission line house distribution graph generating method based on the GIS mode has the advantages that the automatic house numbering method is provided, efficiency is improved and subjective errors are avoided. Names of house correlated photos are stored in the house property, the photos are renamed in batches according to the serial number of the house, the house distribution graph is directly output from the house plane graph, middle files are not needed, and therefore house graph processing is more flexible.
Owner:POWERCHINA FUJIAN ELECTRIC POWER SURVEY & DESIGN INST CO LTD

Detector for detecting visible foreign matter in bottled liquid medicine

The present invention relates to a detector for detecting visible foreign matter in bottled liquid medicines, comprising a base, and a bottle inlet device, a plate rotation device, a light source device, an image capturing device, a bottle clamping and releasing device, and a bottle outlet device fixed on the base. The bottle inlet device is used for advancing the to-be-detected liquid medicine bottles, the liquid medicine bottles are rotated by the plate rotation device to facilitate the image capturing device to detect, the light source device provides light for the liquid medicine bottles, the bottle clamping and releasing device is used for clamping and releasing the liquid medicine bottles, and the detected liquid medicine bottles are removed from the bottle outlet device. The detector is reasonable in structure design, can automatically detect liquid medicine bottles through streamlined operations, does not need artificial detection of foreign matters, prevents subjective errors on artificial detection, and improves work efficiency.
Owner:东营益盟盛药业有限公司

Tank container frame assembly platform system

The invention relates to a tank container frame assembly platform system, which is characterized by mainly comprising a foundation pit, operation platforms, ladders and guard rails, wherein the operation platforms, the ladders and the guard rails are positioned on the two sides of the foundation pit and are parallel to each other; base beams at the front and rear ends of the operation platforms are provided with tank container front and rear frame fixing devices respectively; the base beams are movably provided with fixing support frames for fixing a lower beam of a tank container; cross beams of the operation platforms are movably provided with fixing support frames for fixing an upper beam of the tank container; a horizontal guide rail, a vertical guide rail and a three-dimensional adjusting trolley are arranged in the foundation pit; and the bottom of the three-dimensional adjusting trolley is provided with a hydraulic driving system, a trolley chassis and a rolling support mechanism. Accumulation of subject factor error of a person is avoided in an entire assembly process, the assembly accuracy is improved, and meanwhile, the working efficiency is also greatly improved; and in addition, a tank can also be adjusted and transported to a proper position through the three-dimensional adjusting trolley, so that the precision between a frame and the tank can be guaranteed, and the labor intensity is relieved.
Owner:天津新华昌运输设备有限公司

Environmental sanitation management service system having garbage collection and monitoring functions

The invention discloses an environmental sanitation management service system having garbage collection and monitoring functions. The system comprises a portal sub-system used for providing a unified access module to users so as to realizing environmental sanitation cloud service online ordering and opening; a mobile application sub-system used for providing mobile application service to the users; an office OA sub-system used for realizing a standard OA office function; an ERP sub-system used for realizing optimization of enterprise resource configuration; a larger screen monitoring sub-system used for displaying video information of the management service system; an operation work sub-system used for operation management, standardizing behaviors of workers, and collecting and managing operation data; and a worker supervision and examination system used for refine management of workers. By adopting the environmental sanitation management service system, the information is collected in real time, and the statistic accuracy is high.
Owner:升禾城市环保科技股份有限公司

System for providing environmental sanitation management service

The invention discloses a system for providing environmental sanitation management service, comprising a portal subsystem used for providing a unified access module for users to realize online ordering and opening of environmental sanitation cloud services, a mobile application subsystem used for providing mobile application services for users, an office OA subsystem used for achieving a standard OA office function, an ERP subsystem used for optimizing enterprise resource configuration, a large-screen monitoring subsystem used for displaying video information of a management service system, an operation subsystem used for carrying out operation management, regulating the behaviors of operators and collecting and sorting operation data, and an operator supervision and appraisal subsystem used for fine management of operators. The purpose of real-time information acquisition and high statistical accuracy are achieved.
Owner:升禾城市环保科技股份有限公司

Platform for environmental management

The invention discloses a platform for environmental management. The platform includes a portal subsystem for providing a user with a unified access module to achieve online ordering and opening of a sanitation cloud service; a mobile application subsystem for providing the user with a mobile application service; an OA subsystem for achieving standard OA functions; an ERP subsystem for optimization of enterprise resource allocation; a large screen monitoring subsystem for displaying video information of a management service system; an operation subsystem for managing the operation, standardizing the behavior of operators, and collecting and organizing operation data; and an operator monitoring and assessing subsystem for refined management of the operators, wherein an RFID electronic tag is installed on a trash and is built in with a differential amplification circuit. The purposes of real-time information acquisition and high statistical accuracy are achieved.
Owner:升禾城市环保科技股份有限公司

Log information analysis method and related device

The invention discloses a log information analysis method, comprising the steps: selecting a corresponding log analysis mode according to received mode selection information; performing code generation processing on the received condition information according to the log analysis mode to obtain an analysis rule corresponding to the log analysis mode; analyzing log data to be analyzed according tothe analysis rule to obtain a target log event corresponding to the log data; selecting a corresponding log analysis mode according to the received mode selection information to perform encoding generation processing on the received condition information, obtaining the analysis rule and analyzing the log data, and obtaining the target log event, thus realizing analysis of log information. Therefore, as different log analysis modes can be selected to analyze log data, analysis flexibility is improved. The invention further discloses a log information analysis device, a server and a computer readable storage medium, which have the above beneficial effects.
Owner:SANGFOR TECH INC

Method for automatically judging vehicle door opening and closing state based on video passenger flow

The invention discloses a method for automatically judging vehicle door opening and closing state based on a video passenger flow. In combination with the video passenger flow analysis technology, vehicle front and rear doors can be automatically opened and closed, the subjective errors, for instance, passengers miss the stop because the rear vehicle door is not opened when a vehicle arrives at the station, the overload of the vehicle is serious, and the driving safety is not facilitated, of a driver can be avoided, and the rights and interests of the passengers are guaranteed to the greatest extent.
Owner:ANHUI FUHUANG TECH CO LTD

Medication guidance and gene detection kit for antihypertensive drug irbesartan

The invention provides a medication method and a kit of an antihypertensive drug irbesartan. The method comprises the following steps: respectively designing multiple amplification primers and extension primers according to a plurality of to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in a reaction system, using aplurality of primers for simultaneously and respectively carrying out amplification and single base extension reaction on a plurality of target SNP sites; carrying out flight time mass spectrometry analysis on a product obtained after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by mass spectrometry peaks, and guiding medication of the antihypertensive drug irbesartan. The invention also provides the detection kit using the method. The SNP locus related to metabolism of the antihypertensive drug irbesartan can be detected, and the SNP locus has the advantages of being low in cost, free of probe synthesis, short in consumed time, simple and convenient in result analysis and extremely wide in application field.
Owner:BIOYONG TECH

Gene detection kit for guiding individualized medication of nitrendipine drug for reducing high blood pressure

The invention provides a method and kit for guiding medication of nitrendipine. The method comprises steps as follows: multiplex amplification primers and an extension primer are designed respectivelyaccording to two to-be-detected target SNP sites; a multiplex amplification primer reaction system and an extension primer reaction system are prepared; in the reaction systems, an amplification reaction and a single base extension reaction are performed on the two target SNP sites respectively by using multiple sets of primers; products obtained by the single base extension reaction are subjected to time-of-flight mass spectrometry analysis, according to extension primer products with different molecular weights and represented by mass spectrum peaks, genotypes of different SNPs related to drug metabolism are identified, and medication of the nitrendipine drug for reducing high blood pressure is guided. Besides, the invention also provides a detection kit using the method. The kit can detect two SNP sites related to metabolism of the nitrendipine drug for reducing high blood pressure simultaneously and has the advantages that the kit is low in cost, doesn't need synthesis probe, takes short time and is simple and convenient in result analysis and very broad in application field.
Owner:BIOYONG TECH

Method for carrying out mass-spectrography differentiation on nitrendipine individualized medication through detection product

The invention discloses a method for carrying out mass-spectrography differentiation on nitrendipine individualized medication through a detection product. The method comprises the following steps of:according to two target SNP (single nucleotide polymorphism) sites to be detected, independently designing multiplex amplification primers and an extension primer; preparing a multiplex amplificationprimer reaction system and an extension primer reaction system; in the reaction systems, adopting multiple sets of primers to simultaneously independently carry out an amplification reaction and a single base extension reaction on the two target SNP sites; carrying out time-of-flight mass spectrometry analysis on products obtained by the single base extension reaction; according to extension primer products with different molecular weights and represented by mass spectrometry peaks, identifying the genotypes of different SNPs related to medicine metabolism; and guiding the individualized medication of a hypertension-reducing medicine, i.e., nitrendipine. The method can simultaneously detect the two SNP sites related to nitrendipin metabolism, has the advantages of low cost, short time consumption and an extremely wide application field and does not need to carry out probe synthesis, and result analysis is simple and convenient.
Owner:BIOYONG TECH

Application publishing method and related device

The invention discloses an application publishing method. The method comprises the steps of obtaining a template parameter file through a visual template editor; carrying out template file generationprocessing on the template parameter file according to preset chart template data to obtain a chart template file; and uploading the chart template file to an application management platform and carrying out application release processing to obtain a target application; obtaining a template parameter file through a visual editor; then generating the chart template file through the template parameter file, and finally carrying out application publishing according to the chart template file instead of manually compiling the chart template file, so that subjective errors are avoided while the efficiency is improved, and the threshold of application publishing is reduced. The invention further discloses an application publishing device, a server and a computer readable storage medium, which have the above beneficial effects.
Owner:北京浪潮数据技术有限公司

Environmental sanitation management service system with cleaning sprinkler operation regulation function

The invention discloses an environmental sanitation management service system with a cleaning sprinkler operation regulation function, comprising a portal subsystem used for providing a unified access module for users to realize online ordering and opening of environmental sanitation cloud services, a mobile application subsystem used for providing mobile application services for users, an office OA subsystem used for achieving a standard OA office function, an ERP subsystem used for optimizing enterprise resource configuration, a large-screen monitoring subsystem used for displaying video information of a management service system, an operation subsystem used for carrying out operation management, regulating the behaviors of operators and collecting and sorting operation data, and an operator supervision and appraisal subsystem used for fine management of operators. The purpose of real-time information acquisition and high statistical accuracy are achieved.
Owner:升禾城市环保科技股份有限公司

Detection product for judging individualized medication type of nitrendipine

The invention provides a detection product for judging individualized medication type of nitrendipine. A preparation method comprises steps as follows: multiplex amplification primers and an extensionprimer are designed separately according to two to-be-detected target SNP sites; a multiplex amplification primer reaction system and an extension primer reaction system are prepared; in the reactionsystems, an amplification reaction and a single base extension reaction are performed on the two target SNP sites respectively by using multiple sets of primers; products obtained by the single baseextension reaction are subjected to time-of-flight mass spectrometry, according to extension primer products with different molecular weights and represented by mass spectrum peaks, genotypes of different SNPs related to drug metabolism are identified, and individualized medication of the nitrendipine drug for reducing high blood pressure is guided. The detection product can detect two SNP sites related to metabolism of the nitrendipine simultaneously and has the advantages that the detection product is low in cost, doesn't need probe synthesis, takes short time and is simple and convenient inresult analysis and very broad in application field.
Owner:BIOYONG TECH

Gene detection kit for medicine application instruction of hypertension reduction medicine telmisartan

The invention provides a medicine using method and a kit of hypertension reduction medicine telmisartan. The method comprises the following steps: separately designing multiple amplification primers and extension primers according to a plurality of target SNP locus to be detected; preparing multiple amplification primer reaction systems and extension primer reaction systems; simultaneously performing amplification and single base extension reaction on the plurality of target SNP locus by using multiple sets of primers in the reaction systems; performing flight time mass spectrometry analysis on the product subjected to the single base extension reaction, verifying gene types of metabolism-related SNP of different medicines, and guiding using of the hypertension reduction medicine telmisartan. Meanwhile, the invention provides the detection kit using the method. According to the medicine using method and the kit disclosed by the invention, SNP locus related with the metabolism of the hypertension reduction medicine telmisartan can be detected, and the kit has the advantages of being low in cost, having no need of synthetic probe, being short in time consumption, being simple and convenient in result analysis, and being extremely wide in application field, and can be used for aided diagnosis and treatment of hypertension patients who clinically need oral administration of the telmisartan.
Owner:BIOYONG TECH

System for providing health management service

The present invention discloses a system for providing health management service. The system comprises: a portal subsystem configured to provide a uniform access module for users to realize the online order and opening of an environmental sanitation cloud service; a mobile application subsystem configured to provide a mobile application service for users; an office OA subsystem configured to realize a standard OA office function; an EPP subsystem configured to realize the optimization of the enterprise resource configuration; a large-screen monitoring subsystem configured to display video information of a management service system; an operation work subsystem configured to perform operation management, regulate operating personnel's behaviors and collect and arrange the operation data; and a operating personnel monitoring and examination subsystem configured to perform operating personnel's fine management, wherein a radio frequency identification (RFID) is installed on a dustbin, and the RFID is internally provided with a power amplification circuit. The system for providing environment management service is able to acquire information in real time and has high statistics accuracy.
Owner:升禾城市环保科技股份有限公司

Platform for environmental management

The invention discloses a platform for environmental management. The platform includes a portal subsystem for providing a user with a unified access module to achieve online ordering and opening of a sanitation cloud service; a mobile application subsystem for providing the user with a mobile application service; an OA subsystem for achieving standard OA functions; an ERP subsystem for optimization of enterprise resource allocation; a large screen monitoring subsystem for displaying video information of a management service system; an operation subsystem for managing the operation, standardizing the behavior of operators, and collecting and organizing operation data; and an operator monitoring and assessing subsystem for refined management of the operators, wherein an RFID electronic tag is installed on a trash and is built in with a differential amplification circuit. The purposes of real-time information acquisition and high statistical accuracy are achieved.
Owner:升禾城市环保科技股份有限公司

Mass-spectrography method for identifying personalized medication of nitrendipine through primer composition

The invention provides a detection product for mass-spectrometry identification of personalized medication of nitrendipine through a primer composition. A preparation method of the detection product includes the steps of: designing multiple-amplification primers and extension primers separately according to two to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions on the two target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis on products obtained after the single-base extension reactions, identifying the genotypes of different SNPs related to metabolism of the drug according to extension primer products which are represented by mass spectrum peaks and have different molecular weights, and guiding personalized medication of the antihypertensive drug nitrendipine. The two SNP sites related to metabolism of the nitrendipine drug can be detected simultaneously, and the product has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wide application fields.
Owner:BIOYONG TECH

Gene detection kit for guiding medication of antihypertensive drug nitrendipine and atenolol

The invention provides a method and a kit for guiding medication of nitrendipine and atenolol. The method comprises the following steps: respectively designing multiple amplification primers and extension primers according to 10 to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneouslyand respectively carrying out amplification and single-base extension reaction on the 10 target SNP loci by using a plurality of sets of primers; and carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by a mass spectrum peak, and guiding the medication of antihypertensive drug nitrendipine and atenolol. Meanwhile, the invention provides the detection kit using the method. The methodcan simultaneously detect the 10 SNP loci related to metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.
Owner:BIOYONG TECH

Primer composition for identifying personalized medication of nitrendipine

The invention provides a primer composition for identifying personalized medication of nitrendipine. The preparation method of the primer composition includes the steps: designing multiple-amplification primers and extension primers separately according to two to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions on the two target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis of products obtained after the single-base extension reactions, identifying the genotypes of different SNPs related to metabolism of the drug according to extension-primer products which are represented by mass spectra and have different molecular weights, and guiding personalized medication of the antihypertensive drug nitrendipine. The two SNP sites related to metabolism of the nitrendipine drug can be detected simultaneously, and the product has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wideapplication fields.
Owner:BIOYONG TECH

Gene detection kit for medication guidance of antihypertensive drug lacidipine

The invention provides a method utilizing extension primers with different molecular weights at different SNP (single nucleotide polymorphism) sites to detect multiple sites related to metabolism of an antihypertensive drug lacidipine by MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) and guide medication of lacidipine finally as well as a kit. The method comprises steps as follows: designing multiple amplification primers and extension primers respectively according to six target SNP sites to be detected; preparing a multi-amplification primer reaction system and an extension reaction system; performing amplification and single-base extension reactions on the six target SNP sites by multiple primers simultaneously respectively; performing time-of-flight mass spectrometry on products obtained after the single-base extension reaction, identifying genetypes of SNPs related to different drug metabolisms according to products, represented by massspectrum peaks, of extension primers with different molecular weights, and guiding the medication of the antihypertensive drug lacidipine. Meanwhile, the invention provides a detection kit utilizingthe method. The SNP sites related to metabolism of six antihypertensive drug lacidipine can be detected simultaneously, and the method has the advantages of low cost, no need of probe synthesis, low time consumption, simple and convenient result analysis and quite wide application field.
Owner:BIOYONG TECH

Mass spectrography method for differentiation of individualized medication of lacidipine through primer composition

The invention provides a mass spectrography method for differentiation of individualized medication of lacidipine through a primer composition. According to different single nucleotide polymorphism (SNP) sites having extension primers with different molecular weights, through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), a plurality of sites pertinentto metabolism of a hypertension reducing medicine namely lacidipine can be detected at the same time, and finally the mass spectrography method for differentiation of individualized medication of lacidipine through the primer composition is obtained. The method comprises the steps of: according to 6 target SNP sites to be detected, separately designing multiplex amplification primers and an extension primer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing an amplification reaction and a single-base extension reaction on the 6 target SNP sites at the same time with multiple sets of primers; and performing time-of-flight mass spectrometric analysis on products after the single-base extension reaction,identifying the genotypes of different drug metabolism relevant SNPs according to extension primer products with different molecular weights and represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the lacidipine. The method disclosed by the invention can detect 6 metabolism pertinent SNP sites of the hypertension reducing medicine namely thelacidipine at the same time, and has the advantages of being low in cost, free from probe synthesis, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.
Owner:BIOYONG TECH

Detection product for discriminating individualized medication type of nitrendipine and atenolol

The invention provides a detection product for discriminating the individualized medication type of nitrendipine and atenolol. The method comprises the following steps: respectively designing multipleamplification primers and extension primers according to 10 to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in thereaction systems, simultaneously and respectively carrying out amplification and single-base extension reaction on the 10 target SNP loci by using a plurality of sets of primers; and carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by a mass spectrum peak, and guiding the medication of antihypertensive drug nitrendipine and atenolol. The method can simultaneously detect the 10 SNP loci related to metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.
Owner:BIOYONG TECH

Gene detection kit for guiding administration of antihypertensive drug verapamil

The invention provides a method and kit for guiding administration of verapamil by simultaneously detecting multiple loci related to the metabolism of the antihypertensive drug verapamil through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) because different single nucleotide polymorphism (SNP) loci have extension primers with different molecular weights. The method includes the following steps: designing multiple amplification primers and extension primers according to eight target SNP loci to be detected; preparing a multiple amplification primerreaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single-base extension reactions on the eight target SNP loci by using multiple sets of primers; and performing time-of-flight mass spectrometry analysis on the products after the single-base extension reactions, and identifying the genotypes of different drug metabolism-related SNP loci according to the products of different-molecular-weight extension primers represented by mass spectrum peaks to guide the administration of the antihypertensive drug verapamil. At the same time, the invention provides the detection kit using the method. The method can simultaneously detect the eight SNP loci related to the metabolism of the antihypertensive drug verapamil, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient result analysis, and extremely wide application fields.
Owner:BIOYONG TECH

Method for identifying individualized administration of verapamil by performing mass spectrometry through detection products

The invention provides a method for identifying individualized administration of verapamil by performing mass spectrometry through detection products. The method comprises the following steps: designing multiple amplification primers and extension primers according to eight target SNP loci to be detected; preparing a multiple amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single-base extension reactions on the eight target SNP loci by using multiple sets of primers; and performing time-of-flightmass spectrometry analysis on the products after the single-base extension reactions, and identifying the genotypes of different drug metabolism-related SNP loci according to the products of different-molecular-weight extension primers represented by mass spectrum peaks to guide the administration of the antihypertensive drug verapamil. The method can simultaneously detect the eight SNP loci related to the metabolism of the antihypertensive drug verapamil, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient result analysis, and extremely wide application fields.
Owner:BIOYONG TECH

Detection product for distinguishing individualized medication types of trandolapril

The invention provides a detection product for distinguishing individualized medication types of trandolapril as an antihypertensive drug. A preparation method of the detection product comprises the following steps: designing multiplex amplification primers and extension primers respectively according to a plurality of to-be-detected target SNP (single nucleotide polymorphism) loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and single base extension reactions on the target SNP loci simultaneously and respectively by multiple sets of primers; performing time-of-flight mass spectrometry on a product obtained after the single base extension reaction, identifying genotypes of SNP relatedto metabolism of different drugs, and guiding individualized medication of the trandolapril as the antihypertensive drug. The detection product can detect five SNP loci related to trandolapril drug metabolism simultaneously, has the advantages of low cost, no need of synthetic probes, short consumed time, simple and convenient result analysis and extremely wide application field, and can be usedfor auxiliary diagnosis and treatment of hypertensive patients requiring to orally take the trandolapril clinically.
Owner:BIOYONG TECH

Mass spectrometry method for identifying personalized medication of trandolapril through product detection

The invention provides a mass spectrometry method for identifying personalized medication of trandolapril through product detection. The method includes the steps: designing multiple-amplification primers and extension primers separately according to multiple to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions at the target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis of products obtained after the single-base extension reactions so as to identify the genotypes of SNP related to metabolism of different drugs, and guiding personalized medication of the antihypertensive drug trandolapril. Five SNP sites related to metabolism of the trandolapril drugs can be detected simultaneously, and the method has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wide application fields, and can be applied to auxiliary diagnosis and treatment of hypertensive patients who need to take trandolapril clinically.
Owner:BIOYONG TECH

Medication guiding gene detection reagent kit of hypertension reducing medicine namely trandolapril

The invention provides a medication method and reagent kit of a hypertension reducing medicine namely trandolapril. The medication method comprises the steps of according to multiple objective SNP sites to be detected, respectively designing a multiplex amplification primer and an extension primer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and a single-base extension reaction on the objective SNP sites at the same time with multiple sets of primers; and performing flight time mass spectrometric analysis on products after being subjected to the single-base extension reaction, identifying the genotypes of different drug metabolism relevant SNPs according to products of different molecular weight extension primers represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the trandolapril. Besides, the invention provides a detectionreagent kit using the method. According to the medication method disclosed by the invention, 5 trandolapril drug metabolism pertinent SNP sites can be detected at the same time, and the medication method has the advantages of being low in cost, free from synthetized probes, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.
Owner:BIOYONG TECH
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