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Cosmetic compositions comprising exopolysaccharides derived from microbial mats, and use thereof

Inactive Publication Date: 2011-06-23
LUCAS MEYER COSMETICS CANADA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0017]In another specific embodiment, the composition of the present invention is for use in anti-aging skin care. In another specific embodiment, the composition of the present invention is for use in after sun skin care. In another specific embodiment, the composition of the present invention is for use in sunscreen skin care. In another specific embodiment, the composition of the present invention is for use for preventing or reducing at least one skin aging sign.
[0018]In a specific embodiment, the composition is for improving hydration. In another specific embodiment, the composition is for improving the morphology of stratum corneum. In another specific embodiment, the composition is for improving skin microrelief. In another specific embodiment, the composition is for improving desquamation. In another specific embodiment, the composition is for improving keratinocytes differentiation. In another specific embodiment, the composition is for reducing bacterial adhesion on skin surface. In another specific embodiment, the bacterial strain is Staphylococcus epidermidis or Staphylococcus aureus. In another specific embodiment, the composition is for stimulating hyaluronic acid production by senescent human fibroblasts. In another specific embodiment, the composition is for stimulating epidermis total lipid synthesis. In another specific embodiment, the composition is for stimulating the expression of at least one gene involved in skin desquamation. In another specific embodiment, the gene is kallikrein 5, neurosin or stratum corneum chymotrypsic enzyme. In another specific embodiment, the composition is for stimulating the expression of at least one gene involved in keratinocytes differentiation. In another specific embodiment, the gene is filaggrin, loricrin or involucrin. In another specific embodiment, the composition is for stimulating the expression of transglutaminase. In another specific embodiment, the composition is for reducing intracellular lipid peroxides of irradiated skin cells.
[0020]In accordance with another aspect of the present invention, there is provided a use of at least one exopolysaccharide (EPS) originating from a microbial mat, for preventing or reducing at least one skin aging sign.
[0021]In a specific embodiment, the use is for improving hydration. In another specific embodiment, the use is for improving the morphology of stratum corneum. In another specific embodiment, the use is for improving skin microrelief. In another specific embodiment, the use is for improving desquamation. In another specific embodiment, the use is for improving keratinocytes differentiation. In another specific embodiment, the use is for reducing bacterial adhesion on skin surface. In another specific embodiment, the bacterial strain is Staphylococcus epidermidis or Staphylococcus aureus. In another specific embodiment, the use is for stimulating hyaluronic acid production by senescent human fibroblasts. In another specific embodiment, the use is for stimulating epidermis total lipid synthesis. In another specific embodiment, the use is for stimulating the expression of at least one gene involved in skin desquamation. In another specific embodiment, the gene is kallikrein 5, neurosin or stratum corneum chymotrypsic enzyme. In another specific embodiment, the use is for stimulating the expression of at least one gene involved in keratinocytes differentiation. In another specific embodiment, the gene is filaggrin, loricrin or involucrin. In another specific embodiment, the use is for stimulating the expression of transglutaminase. In another specific embodiment, the use is for reducing intracellular lipid peroxides of irradiated skin cells.
[0022]In accordance with another aspect of the present invention, there is provided a method of preventing or reducing a skin aging sign in a subject, comprising administering a composition comprising an effective amount of at least one exopolysaccharide (EPS) originating from a microbial mat on the subject's skin, whereby the skin aging sign is prevented or reduced.

Problems solved by technology

It is the alteration of the barrier properties and actual damage to this barrier that causes skin conditions.
Alteration of this layer will affect negatively perceived quality of the skin and will lead eventually to cutaneous aging.
In the absence of water, the cells do not desquamate normally and the result is thickened, dry, rough, scaly skin.
Exposure to irritants compromises the barrier function of the stratum corneum and decreases its ability to protect the skin against environmental stresses (e.g., ultraviolet irradiation, infections agents, etc.).
Repeated and prolonged exposition to environmental irritants results in denatured skin proteins, disorganization of the lipid lamellae layers, removal of the protective intercellular lipids, loss of natural moisturizing factors and decreased cohesion between cells.
There are also endogenous factors that make one susceptible to damaged skin by external factors.

Method used

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  • Cosmetic compositions comprising exopolysaccharides derived from microbial mats, and use thereof
  • Cosmetic compositions comprising exopolysaccharides derived from microbial mats, and use thereof
  • Cosmetic compositions comprising exopolysaccharides derived from microbial mats, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Effect of EPS on Human Keratinocytes (Viability In Vitro)

[0141]The cytotoxicity evaluation of each EPS was performed by using the MTT assay in order to determine the concentrations which are not harmful to human keratinocytes (NHEK, 3rd passage).

[0142]The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. The assay is based upon the capacity of the mitochondrial dehydrogenase to reduce the yellow tetrazolium MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide), to generate reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilised and quantified by spectrophotometric means. The results are given in Table IV below.

TABLE IVCell's viabilityConcentration% of MTTEPS preparation(mg / mL)conversionPol-10.056980.0181020.00698Pol-20.111970.0371030.012101Pol-30.037950.012970.004101Pol-40.056950.0181010.00698Pol-50.0371...

example 2

Effect of EPS on Senescent Human Fibroblasts (Viability In Vitro)

[0144]The cytotoxicity evaluation of each compound was performed by using the MTT assay as described in Example 1 above, in order to determine the concentrations which are not harmful to senescent human fibroblasts. These senescent cells were obtained by subcultures of normal dermal fibroblasts and a senescent phenotype was obtained from the 12th passage. Table V below provide viability results.

TABLE VCell's viabilityConcentration% of MTTEPS preparation(mg / mL)conversionPol-10.1671010.0561040.018108Pol-20.333990.1111070.037104Pol-30.3331020.1111050.037105Pol-40.5001120.1661180.055117Pol-50.3331040.1111160.037117Pol-60.111940.037950.01293Pol-70.1671040.0551000.018100Pol-80.500990.1661120.055112Pol-90.1111050.0371020.01295

[0145]No viability alteration was observed at the polysaccharide concentrations tested in this assay.

example 3

Effect of EPS on Hyaluronic Acid Synthesis by Senescent Human Fibroblasts

[0146]This study has been performed in order to evaluate the effects of the EPSs on the synthesis of hyaluronic acid by senescent human fibroblasts. Hyaluronic acid synthesis is a marker of fibroblasts activity and this compound is a natural skin humectant, as it possesses hygroscopic properties. These senescent cells are obtained by subcultures of normal dermal fibroblasts and a senescent phenotype is obtained from the 12th passage.

[0147]Gene expression analysis of EPSs tested demonstrated a decrease of synthesis of extracellular matrix proteins, a decrease in sensitivity to growth factors, and an increase of synthesis of matrix metalloproteinases. Some compounds having no direct effect on cell growth have been demonstrated to stimulate proliferation of senescent fibroblasts in presence of epidermal growth factor (EGF). This effect was attributed to an increase in sensitivity of cells to EGF. Based on this obs...

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Abstract

Disclosed herein is a skin care composition comprising at least one exopolysaccharide (EPS) originating from a microbial mat, wherein the EPS is in a concentration of about 0.001% w / w to about 1.5 w / w of the composition. Preferably, the EPS is derived from microorganism isolated from microbial mats found in French Polynesia. The composition is useful in reducing and preventing signs of skin aging and environmental damage by altering skin cell metabolism and improving hydration.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority, under 35 U.S.C. §119(e), of U.S. provisional application Ser. No. 61 / 044,992, filed on Apr. 15, 2008. All documents above are incorporated herein in their entirety by reference.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]N / A.FIELD OF THE INVENTION[0003]The present invention generally relates to cosmetic compositions and methods of use thereof.BACKGROUND OF THE INVENTION[0004]Skin is a physical barrier to the environment. It is the alteration of the barrier properties and actual damage to this barrier that causes skin conditions.[0005]The epidermis and the dermis, separated by the basal membrane, constitute the cutaneous covering on the hypoderm. The epidermis is the most superficial layer of the skin and provides its resistance and impermeability. Alteration of this layer will affect negatively perceived quality of the skin and will lead eventually to cutaneous aging. The dermis, ...

Claims

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Application Information

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IPC IPC(8): A61K8/73A61Q17/04
CPCA61K8/73A61Q19/08A61K31/715A61K8/99A61Q19/00A61K2800/74A61P17/00C12R2001/45C12N1/205C12R2001/445
Inventor LOING, ESTELLEBRIATTE, SANDRINEVAYSSIER, CATHERINEBEAULIEU, MARTINDIONNE, PATRICERICHERT, LAURENTMOPPERT, XAVIER
Owner LUCAS MEYER COSMETICS CANADA
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