Method for diagnosing active mud-ratio electronic transferring system
An electron transfer and activated sludge technology, applied in the field of water supply and drainage, can solve the problems of health hazards, accuracy effects, and inability to truly characterize the activity of the electron transfer system.
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[0014] 1. Clean the test tube with pure water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;
[0015] 2. Add 0.3mL of activated sludge mixture, 1.5mL of Tris-HCl buffer solution and 1mL of 0.2% INT solution to the 10mL test tube in sequence;
[0016] 3. Quickly place the prepared sample in a water bath shaker at (37±1)°C, shake and cultivate in the dark for 30 minutes;
[0017] 4. When the incubation time is up, add 1mL of 37% formaldehyde solution to the test tube to terminate the reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;
[0018] 5. Add 5mL of methanol to the sample, mix and stir evenly, shake and extract in the dark at (37±1)°C for 10min;
[0019] 6. Centrifuge the extracted sample at 4000r / min for 5min, then measure the absorbance of the INTF extract of the sample to be tested at 485nm of the 721-spectrophotometer with the sample added with 3 drops of m-cresol as a blank control;
[0020] 7. Dry the sepa...
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