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Method for diagnosing active mud-ratio electronic transferring system

An electron transfer and activated sludge technology, applied in the field of water supply and drainage, can solve the problems of health hazards, accuracy effects, and inability to truly characterize the activity of the electron transfer system.

Inactive Publication Date: 2007-06-20
尹军 +1
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Problems solved by technology

The scope of application of TTC is limited, and it is not suitable for detecting the biological activity of anaerobic and denitrifying denitrification sludge; when TTC is used to detect the activity of the electron transfer system of activated sludge, acetone, toluene and acetone + tetrachloroethylene (2 +3) As the extraction agent of TF, these extraction agents have different degrees of harmful effects on the health of experimental operators, especially tetrachlorethylene is also a suspected carcinogen; the TF extraction process is often completed at high temperature (90°C) , resulting in partial evaporation of the extractant, thereby affecting the accuracy of the experimental results; the activity of the activated sludge electron transport system detected by TTC is often expressed in units of the number of TFs produced per unit volume of mixed solution per unit time. The real characterizing unit is the activity of the electron transfer system of the activated sludge in dry weight, that is, the activity of the specific electron transfer system, and in the case of different concentrations of the mixed solution, the accuracy of the experimental results expressed by this unit is also seriously affected

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  • Method for diagnosing active mud-ratio electronic transferring system
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  • Method for diagnosing active mud-ratio electronic transferring system

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Embodiment Construction

[0014] 1. Clean the test tube with pure water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;

[0015] 2. Add 0.3mL of activated sludge mixture, 1.5mL of Tris-HCl buffer solution and 1mL of 0.2% INT solution to the 10mL test tube in sequence;

[0016] 3. Quickly place the prepared sample in a water bath shaker at (37±1)°C, shake and cultivate in the dark for 30 minutes;

[0017] 4. When the incubation time is up, add 1mL of 37% formaldehyde solution to the test tube to terminate the reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;

[0018] 5. Add 5mL of methanol to the sample, mix and stir evenly, shake and extract in the dark at (37±1)°C for 10min;

[0019] 6. Centrifuge the extracted sample at 4000r / min for 5min, then measure the absorbance of the INTF extract of the sample to be tested at 485nm of the 721-spectrophotometer with the sample added with 3 drops of m-cresol as a blank control;

[0020] 7. Dry the sepa...

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Abstract

A method for diagnosing activity of activated sludge ratio electronic transmission system includes utilizing test tube to determine dry weight of sludge, using iodinenitryl tetraazole as electronic receptor and using methanol as excctant to extract out INTF in microbiological cell under ambient temperature.

Description

technical field [0001] The invention belongs to the technical field of water supply and drainage, and in particular relates to a method for diagnosing the activity of activated sludge. Background technique [0002] The microbial activity of activated sludge is an important indicator for analyzing, evaluating and predicting the performance of sewage biological treatment. It can not only reflect the ability of microorganisms to degrade organic substances in the treatment system from the perspective of molecular biology, but also measure the degradation rate of organic substances from the level of biological activity. and the performance of biological treatment facilities. The sewage treatment plants in my country are dominated by biological treatment methods. According to the biological activity analysis results of the sewage biological treatment system, the biological treatment performance can be monitored online, the biological activity status of sewage treatment microorgani...

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Application Information

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IPC IPC(8): G01N21/78C12Q1/02C02F11/02
Inventor 尹军谭学军张立国唐利王建辉王雪峰
Owner 尹军
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