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Blended self-assembly membrane based micro ampere immunity sensor and preparation thereof

A hybrid self-assembly and immune sensor technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of reducing the effective load rate of antibodies on the electrode surface, reducing the detection sensitivity of immune sensors, and less commercial applications of immune sensors. Low cost of mass production, good spatial distribution, and the effect of maintaining immune activity

Inactive Publication Date: 2007-02-07
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

However, despite a large number of studies and reports in related fields, the commercial application of immunosensors in clinical diagnosis is very small, mainly due to several basic problems that have not been well resolved: immune biomolecules (antigen / Antibody) has a low loading rate on the electrode, it is difficult to control the orientation of the immobilized antibody and maintain the immunological activity of the immobilized antibody
However, immobilizing antibodies on a self-assembled monolayer of a single component tends to form a large steric hindrance between antibodies, which hinders the specific recognition and binding of the immunologically active site of the antibody to the antigen in the analysis sample, and reduces the ability of the antibody on the electrode surface. effective load rate, thereby reducing the detection sensitivity of the immunosensor

Method used

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  • Blended self-assembly membrane based micro ampere immunity sensor and preparation thereof
  • Blended self-assembly membrane based micro ampere immunity sensor and preparation thereof
  • Blended self-assembly membrane based micro ampere immunity sensor and preparation thereof

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Embodiment

[0035] The following uses the measurement of immunoglobulin G (IgG) as an example to illustrate the miniature amperometric immunosensor based on the mixed self-assembled membrane and its preparation method. The clinical significance of IgG is associated with a variety of congenital and autoimmune diseases.

[0036] 1. Miniature amperometric immunosensor based on hybrid self-assembled membrane, as shown in Figure 1:

[0037] The sensor is prepared by micro-nano processing technology, including a silicon substrate 1, a silicon oxide and silicon nitride insulating layer 2, and a circular gold film working electrode 3 concentrically distributed from the inside to the outside on the silicon oxide and silicon nitride insulating layer 2 , the annular sensitive film pool 4 surrounded by SU-8 photoresist, the annular platinum thin film counter electrode 5, the annular platinum thin film reference electrode 6, the annular reaction pool 7 surrounded by SU-8 photoresist, and the circular ...

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Abstract

The micro-amp immunity sensor based on mixed self-assembly film comprises: a silicon substrate, a SiO2 and Si3N4 insulation layer, and the concentric circular gold film electrode, SU-8 photoresist ring sensitive film pond, a ring Pt-film counter electrode / reference electrode, a Su-8 photoresist ring reaction pond, and a bio-molecular sensitive film fixed on the electrode. With nano technology and mixed self-assembly film, this invention has well consistence, correlativity and compatibility with IC, and shows a new path for immunity analysis and detection.

Description

technical field [0001] The invention relates to an immunosensor and its preparation, in particular to a miniature amperometric immunosensor based on a mixed self-assembled membrane, its preparation and its application in the fields of immune analysis, disease detection and the like. Background technique [0002] Immunoassay is a highly sensitive and highly selective method for the determination of trace substances using antigen / antibody reactions, which provides a powerful means for the clinical immunoassay of immune biomolecules and tumor markers. Conventional clinical immunoassay methods mainly include: radioimmunoassay, enzyme-linked immunoassay, fluorescence immunoassay, chemiluminescence immunoassay, etc. Among them, radioimmunoassay has high sensitivity, less interference, and wide application range, but the reagent life is limited, and its radioactive markers are harmful to human body; enzyme-linked immunoassay, fluorescence and chemiluminescence immunoassay have larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N27/30H01L49/00H10N99/00
Inventor 夏善红许媛媛边超陈绍凤
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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