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Process for preparing yelk antibody with high bioactivity

A technology of egg yolk antibody and biological activity, which is applied in the field of preparation of highly biologically active egg yolk antibody, can solve the problems of egg yolk antibody activity loss, residual chemical harmful substances, high cost, etc., and achieve improved antibacterial ability, high biological activity, and increased potency Effect

Active Publication Date: 2006-10-11
NANTONG KANGDE BIOLOGICAL PROD
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Problems solved by technology

The first is that the cost is high (reagent consumption is large, such as the amount of PEG-6000 commonly used at present can reach a high concentration of 12%); the second is that the process is complicated, the operating conditions are difficult to master, and it is easy to cause the loss of egg yolk antibody activity; the third is The final product has chemical harmful substances residues, and the safety of the product does not meet the requirements
(2) Using ion exchange, metal chelation chromatography, and affinity chromatography separation methods, although the purity is high, the production scale is only in grams, and the cost can be as high as hundreds of dollars per gram
(3) The aqueous phase extraction method using macromolecular glues such as CMC, sodium alginate and chitosan, because the macromolecular glue is directly added to the egg yolk system, the extraction efficiency is low, and still contains more Impurities and residual colloidal macromolecules such as CMC and sodium alginate directly affect the next ultrafiltration separation and concentration process

Method used

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  • Process for preparing yelk antibody with high bioactivity

Examples

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Effect test

Embodiment 1

[0021] After sterilizing, beating and separating 10 tons of fresh eggs, the obtained egg whites are spray-dried or fermented by microorganisms to obtain about 600 kg of by-product protein powder or desugared protein powder, and about 2.3 tons of obtained egg yolks are added with 25-45 tons of pure water , stirred for 15 minutes, precipitated for 4 to 16 hours, and centrifuged. The precipitate is the remaining egg yolk, and the by-product egg yolk powder is obtained by spray drying, about 1200 kg. The supernatant is the crude extract of egg yolk antibody, and the food-grade additives xanthan gum and calcium lactate are added according to the ratio, stirred for 15 minutes, filtered through a plate frame, and the precipitate is discarded. The clarified liquid is ultrafiltered by POS membrane, 4~12℃, membrane area 100m 2 . After the concentrated retentate is sterilized by 0.22μm PS membrane or ceramic microfiltration membrane, it is then frozen to -40~-30℃, kept for about 120min...

Embodiment 2

[0023] The immunization method of laying hens: the vaccines for immunization are common pathogenic bacteria or viruses in the human oral cavity and gastrointestinal tract: pathogenic Escherichia coli, Salmonella, Pseudomonas aeruginosa, Streptococcus mutans or gastric Helicobacter pylori. After the dose is made into a vaccine, the laying hens aged 20 to 25 weeks are immunized 4 to 6 times in total.

[0024] Take 10 tons of fresh eggs produced by laying hens immunized by the above method as raw materials, and add food additives carrageenan and calcium chloride to the obtained yolk antibody crude extract according to the ratio. The rest of the operations are the same as in Example 1. The main product obtained is high biological The biological titer of the active egg yolk antibody obtained from common eggs in Example 1 is 16-64 times higher.

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Abstract

This invention is preparation procedure of the yolk antibody of high biological activity, belonging to the field of bioproduct and industrialized isolation technique. In this invention, we adopt the natural macromolecule gel high -extractive technique and hyperfiltration to extract the yolk-antibody of high biological activity from yolk at an industrialized level, annual output is upto 10 ton. the yolk-antibody content is about 30%-50%, meanwhile we could obtain some by-products like albumen powder, yolk powder, egg oil, vitellin and so on. In the invention high technology like bioimmunology and membrane separation is adopted. The invention is high-tech, high-usage, highly-automated and typical of green food and environmental-friendly.

Description

technical field [0001] The invention discloses a method for preparing egg yolk antibody with high biological activity, which belongs to the field of biological products and industrial separation technology. Background technique [0002] Chicken yolk is rich in biologically active proteins such as yolk antibody and phosphoprotein, and is considered to be the resource with the highest content and activity of active antibodies and the cheapest resource. But so far, it has not been used industrially as expected. The main reason is that the yolk is rich in fat, protein, cholesterol, lecithin and other substances, which have caused serious difficulties in the industrial separation of yolk antibodies. At present, the methods for isolating yolk antibody mainly include the following: (1) The method of separating organic reagents such as ethanol, polyethylene glycol, acetone, etc., although the separation effect is better, but because yolk antibody is mainly used for functional food ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/02
Inventor 杨严俊徐榕榕
Owner NANTONG KANGDE BIOLOGICAL PROD
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