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Process for biological enzyme extraction of grape seed polypeptide protein

A bio-enzyme method and grape seed technology, applied in the field of plant protein extraction technology, to ensure product quality, reduce costs, and facilitate digestion and absorption

Inactive Publication Date: 2005-06-29
通化腾龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After inquiry, there is no report of extracting polypeptide protein from grape seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] Bio-enzymatic extraction of grape seed polypeptide protein process:

[0035] 1. Raw materials: Dried grape seeds are free from impurities, mildew and insects.

[0036] 2. Crush to 20-40 mesh, separate shell and kernel.

[0037] 3. The grape seeds are crushed into 100 meshes, and 10 times of purified demineralized water is added.

[0038] 4. Pretreatment: The purpose of pretreatment is to destroy the substances in the protein solution that can destabilize the enzyme through high temperature 95-100°C, which is beneficial to the action of endonuclease and accelerates protein hydrolysis. When the protein solution is rapidly cooled to 45-50°C, enzymes are added for hydrolysis for later use.

[0039] 5. Add endo-enzyme hydrolysis: Add slightly acidic cellulase (slightly acidic crude protease) 5‰, and at the same time add endo-papain 3‰, hydrolyze at 45-50°C for 2 hours.

[0040] 6. Add exoprotease for hydrolysis: add exoprotease Aspergillus oryzae 3‰, hydrolyze for 2 hours...

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Abstract

The invention relates to a plant protein extraction process technology, that is, a process for extracting grape seed polypeptide protein by biological enzyme method. The grape seed is used as a raw material, crushed, softened with water, hydrolyzed with endo-cut slightly acidic cellulase and papain mixed enzyme, exo-cut rice koji enzyme hydrolyzed, separated, concentrated and dusted to obtain powdery grape seed polypeptide protein. It has the characteristics of no peculiar taste, high protein extraction rate, low production cost, and maximum protection of raw materials without loss of nutrition during processing.

Description

technical field [0001] The invention relates to a plant protein extraction process technology, that is, a process for extracting grape seed polypeptide protein by a biological enzyme method. Background technique [0002] In the prior art, grape seeds are leftovers in the production process of wineries and grape beverage factories, and are a natural functional food main resource rich in nutrients and having a wide range of sources. Grape seed kernels are rich in protein, which accounts for 28.38% of grape seeds and is of high quality. After inquiry, there is no report on extracting polypeptide protein from grape seeds. Contents of the invention [0003] The object of the present invention is to provide a kind of grape seed as raw material, process is advanced, and the technology of high-quality polypeptide protein is extracted grape seed polypeptide protein by biological enzymatic method aiming at above-mentioned deficiency. [0004] The technical solution of the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00
Inventor 张相明逄风光庄严
Owner 通化腾龙生物科技有限公司
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