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Molecular cngram resin and prepartion method, and application for separating and purifying protein

A molecular imprinting and resin technology, applied in the field of protein separation and purification, can solve the problem of difficult to remove template imprinting effect, and achieve the effect of avoiding damage, strong visibility and strong practicability

Inactive Publication Date: 2005-10-26
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to propose a molecularly imprinted resin and its preparation method and its application for separating and purifying proteins, so as to overcome the shortcomings of the traditional embedding method, which is difficult to remove the template when imprinting proteins, and the surface imprinted protein method. A hydrophilic auxiliary recognition chain with randomly distributed functional groups is introduced into the recognition system. The auxiliary recognition chain and the template protein are first self-assembled, and then the assembled body and free monomer acrylamide are combined with polyacrylate macroporous resin Carry out cross-linking polymerization in aqueous phase as carrier

Method used

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  • Molecular cngram resin and prepartion method, and application for separating and purifying protein
  • Molecular cngram resin and prepartion method, and application for separating and purifying protein
  • Molecular cngram resin and prepartion method, and application for separating and purifying protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Add 53mL of vinyl acetate, 490mL of methanol, and 0.5g of azobisisobutyronitrile into the reaction flask, pass nitrogen and remove oxygen, and react at 70°C for 4h. Add 4.3 g of 40% sodium hydroxide solution to the methanol solution of 10% polyvinyl acetate prepared above, and alcoholyze at 30° C. for 3 h. After centrifugation, vacuum-dry at room temperature for 24 hours to obtain polyvinyl alcohol (PVA).

[0029] Dissolve 2g of polyvinyl alcohol in 18mL of water, add 2mL of dimethylformamide, and pass N at 40°C 2 After 50 minutes, 0.9 mL of nitric acid solution of ammonium cerium nitrate was added, after 15 minutes, 1.94 g of acrylamide was added, after 15 minutes, 1.43 g of 4-vinylpyridine was added, and the reaction was carried out under nitrogen protection at 45°C for 10 hours. Precipitate, separate, and dry in a vacuum oven for 24 hours to obtain PVAVP, a copolymer of polyvinyl alcohol grafted with acrylamide and 4-vinylpyridine.

[0030] Dissolve 1 g of PVAVP in...

Embodiment 2

[0068] Add 16 mL of vinyl acetate, 264 mL of methanol, and 0.15 g of azobisisobutyronitrile into the reaction flask, pass nitrogen and remove oxygen, and react at 65°C for 6 hours. Add 5.73 g of 40% sodium hydroxide solution to the methanol solution of 13% polyvinyl acetate prepared above, and alcoholyze at 35° C. for 3 h. After centrifugation, vacuum-dry at room temperature for 24 hours to obtain polyvinyl alcohol (PVA).

[0069] Polyvinyl alcohol (PVA) 1g, dissolved in 20ml of water, 35 ° C N 2 After 50 minutes, 0.45 mL of nitric acid solution of ammonium cerium nitrate was added, after 15 minutes, 1.04 g of acrylamide was added, after 15 minutes, 0.843 g of 4-vinylpyridine was added, and the reaction was carried out under nitrogen protection at 40°C for 24 hours. Precipitate, separate, and dry in a vacuum oven for 24 hours to obtain PVAVP, a copolymer of polyvinyl alcohol grafted with acrylamide and 4-vinylpyridine.

[0070] Dissolve 0.8g of PVAVP in 15mL of dimethyl sulf...

Embodiment 3

[0080] Add 53mL of vinyl acetate, 574mL of methanol, and 0.65g of azobisisobutyronitrile into the reaction flask, pass nitrogen and remove oxygen, and react at 60°C for 10h. Add 4.78 g of 40% sodium hydroxide solution to the 8% methanol solution of polyvinyl acetate prepared above, and alcoholyze at 30° C. for 2 h. After centrifugation, vacuum-dry at room temperature for 24 hours to obtain polyvinyl alcohol (PVA).

[0081] Polyvinyl alcohol (PVA) 4g, dissolved in 20ml of water, add 5mL of dimethylformamide, 35 ℃ pass N 2 After 50 minutes, 1.10 mL of nitric acid solution of ammonium cerium nitrate was added, after 15 minutes, 3.92 g of acrylamide was added, after 15 minutes, 2.89 g of 4-vinylpyridine was added, and the reaction was carried out under nitrogen protection at 35°C for 36 hours. Precipitate, separate, and dry in a vacuum oven for 24 hours to obtain PVAVP, a copolymer of polyvinyl alcohol grafted with acrylamide and 4-vinylpyridine.

[0082] Dissolve 2g of PVAVP in...

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Abstract

The present invention relates to a molecular imprinting resin, its preparation and application for separation and purification of protein. Its aperture is 2-3 micrometers, and it is prepared by using acrylamide as monomer, using grafts of low molecular weight polyethanol as auxiliary identification chain, using bovine serum albumin as template, using N,N-methylenebisacrylamide as cross-linking agent, using acrylic ester macroporous resin spheres as carrier in phosphate baffer system and using ammonium peroxydisulfate-sodium sulfite oxidation reduction system as initiator through cross-linking polymerization for 4-6 h. at 4-10 deg.C.

Description

technical field [0001] The present invention relates to the synthesis and application of imprinted resin, especially a kind of molecular imprinted resin and its preparation method and its application of separating and purifying protein, using the limited length polymer chain to assist recognition to synthesize protein imprinting polymer, specifically for protein Separation and purification. Background technique [0002] Molecular imprinting technology is the specific recognition ability at the molecular level that polymers possess by "memorizing" the three-dimensional space of imprinted molecules. Due to the particularity of protein structure and the requirement of biological activity, imprinted protein has become a research hotspot in the field of molecular imprinting in recent years. Using non-covalent interactions is the easiest way to imprint proteins. Commonly used weak intermolecular forces include: hydrogen bonds, electrostatic forces, charge transfer, hydrophobic i...

Claims

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Application Information

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IPC IPC(8): C08F261/04
Inventor 宓怀风郭敏杰范云鸽
Owner NANKAI UNIV
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