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Thymosin alpha-1 microball prepn and its prepn process

A technology of thymosin and microspheres, which is applied in the field of medicine, can solve the problems of single administration route, high storage and use conditions, etc., and achieve the effects of improving protein stability, easy storage, and ensuring protein activity

Inactive Publication Date: 2003-02-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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Problems solved by technology

At present, there is only one dosage form of Tα1 preparation——powder injection for injection, which has a single route of administration, which is subcutaneous injection, and requires relatively high conditions during storage and use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach ： Embodiment 1

[0015] Specific embodiments: Example 1: Preparation of Tα1 microspheres

[0016] Using Span80 as emulsifier, emulsify 3~5mg Tα1 in 10ml of dichloromethane solution containing 10% PLGA, control the preparation temperature at 15±2°C to obtain Tα1 emulsion; slowly drop the prepared Tα1 emulsion under stirring Pour into the aqueous solution of 2% PVA, stirring speed is 800rpm; After stirring for 10 minutes, pour 500ml distilled water into this system again, continue to stir, the organic solvent is evaporated to form drug-containing microspheres. The microspheres were collected by centrifugation, washed three times with 60 ml of distilled water, and freeze-dried to obtain 2.5 g of microspheres. The particle diameter of the microsphere is 75-100 μm, and the encapsulation efficiency is 20.8%.

[0017] Animal experiment: Choose a small white pig of about 15kg as the experimental animal. After anesthesia, use a constant temperature electric scald instrument at 75±2°C for 10 seconds to...

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Abstract

The present invention relates to medical technology field and is thymosin alpha-1 microball preparation as a new dosage form of thymosin alpha-1 (Ta1) and its preparation process. Tal is one kind of polypeptide with immunologic function and is used usually in treating viral hepatitis. In the present invention, Ta1 is prepared into protein microball preparation for easy storage and easy use. The present invention can ensure the bioactivity of Ta1 while making it possible to administrate in different ways, such as wound, oral taking and channel.

Description

Technical field: [0001] The invention relates to the technical field of medicine, and relates to a new dosage form of thymosin α1-microsphere preparation and a preparation method thereof. Background technique: [0002] Thymosin α1 (thymosin α1, referred to as Tα1) is a polypeptide with immune function, which is mostly used in clinical treatment of viral hepatitis, and its amino acid sequence is NH 2 -Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu -Ala-Glu-Asn-COOH [Goldstein A L, Low T L, McAdoo M, McClure J, Thurman G B, Rossio J, Lai C Y, Chang D, Wang S S, Harvey C, Ramel A H, Meienhofer. J. Thymosin alpha 1: isolation and sequence analysis of an immunological active thymic peptide. Proc Natl Acad Sci USA, 1977, 74(2): 725-729]]. At present, there is only one dosage form of Tα1 preparation—powder injection for injection, which has a single route of administration, which is subcutaneous injection, and requires relative...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K38/42A61P1/16A61P17/02A61P31/12A61P35/00A61P37/00A61P37/02
Inventor 郭葆玉苗红章杰袁鹏群道书艳邱磊张冉
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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