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Method for separating and purifying ceramide using molecular ungram method

A separation and purification and molecular imprinting technology, applied in the field of separation and purification of ceramide, can solve the problems of long cycle, low processing capacity, complicated separation process, etc., and achieve the effects of low cost, continuous operation, and simple separation and purification process.

Inactive Publication Date: 2006-04-19
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the separation and purification of ceramide by thin layer chromatography requires pretreatment processes such as extract hydrolysis and chromatographic crude separation. The separation process is cumbersome, long cycle, and low throughput.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh the hydrophobic monomer styrene, the hydrophilic monomer glycidyl methacrylate, methacrylic acid and acryloyl-β-cyclodextrin (1:0.5:0.5:0.01mol / mol / mol / mol), Crosslinking agent divinylbenzene and trimerized isocyanurate triallyl urate (1: 1mol / mol, crosslinking agent / monomer 0.5: 1mol / mol); Porogen pyridine (porogen / reaction mixture 75vol% ); initiator azobisisobutyronitrile (initiator / monomer 0.01: 1mol / mol); imprinted molecule ceramide standard sample (imprinted molecule / monomer 0.01: 1mol / mol), mixed evenly, and loaded into 100×5.0 mm glass tube, seal the other end, and react at 65°C for 24 hours. The polymer monolith was removed, ground, extracted with ethanol, and dried. Pack the chromatographic medium with a particle size of 37-74 microns into a 150×4.6 mm chromatographic column and connect it to the chromatographic instrument. Utilize Bligh-Dyer method, extract lipid substance from yeast, (Bligh-Dyer method is that the volume ratio is 2: 1 mixed solution ...

Embodiment 2

[0027] Weigh the hydrophobic monomer styrene, hydrophilic monomer acrylic acid and single methacryloyl-β-cyclodextrin (0.5:1:0.1mol / mol / mol / mol), cross-linking agent divinylphenylmethane And trimethylol propane trimethacrylate (1: 1mol / mol, crosslinking agent / monomer 1: 1mol / mol), porogen dimethylformamide (porogen / reaction mixture 60vol%), trigger Azobisisobutyronitrile (initiator / monomer 0.005: 1mol / mol), imprinted molecular ceramide standard sample (imprinted molecule / monomer 0.1: 1mol / mol), mix well, and put them into a 100×5.0mm glass The other end of the tube was sealed and reacted at 75°C for 12 hours. The polymer monolith was removed, ground, extracted with ethanol, and dried. Pack the chromatographic medium with a particle size of 37-74 microns into a 150×4.6 mm chromatographic column and connect it to the chromatographic instrument. Using the Bligh-Dyer method, the lipids were extracted from the yeast with a 2:1 volume ratio of chloroform and methanol mixed solutio...

Embodiment 3

[0029] Weigh the reaction monomers styrene, hydroxypropyl methacrylate, methacrylic acid and pentasubstituted methacryloyl-β-cyclodextrin (0.5:1:1:0.025mol / mol / mol / mol), cross-linked agent allyl methacrylate and trivinylbenzene (1:2mol / mol, crosslinking agent / monomer 20:1mol / mol), porogen dimethyl sulfoxide (porogen / reaction mixture 40vol%), Initiator dibenzoyl peroxide (initiator / monomer 0.02: 1mol / mol), imprinted molecular ceramide standard sample (imprinted molecule / monomer 1: 1mol / mol), mix well, and pack into 100×5.0mm In a glass tube, the other end was sealed and reacted at 85° C. for 12 hours. The polymer monolith is removed, ground, extracted and dried. Pack the chromatographic medium with a particle size of 37-74 microns into a 150×4.6 mm chromatographic column and connect it to the chromatographic instrument. Using the Bligh-Dyer method, the lipids were extracted from the yeast with a 2:1 volume ratio of chloroform and methanol mixed solution. After drying, the ext...

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PUM

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Abstract

The invention is a method that adopts molecular print to separate and purify nervous amide, mixing the hydrophilic methyl acrolic acid monomers: methyl-acrylate, acrylic acid or one or two of acrylates with the hydrophobic monomer phenylethene and super-molecule monomer, then mixing with the cross-linking agent, the hole-making agent, the evocating agent and the sample of molecule-printed nervous amide, polymerizing, and getting rid of hole-making agent and template molecules to get the molecule-printed separating medium; adopting organic solvent to extract esters; add the esters in chromatogram column with the medium to realize the separation and purification.

Description

technical field [0001] The invention relates to a method for separating and purifying ceramide, which belongs to the separation and purification technology of ceramide. Background technique [0002] The English name of ceramide is Ceramide, and its chemical structure is N-fatty acyl (nervous) sphingosine. It is high in the stratum corneum of the skin and has barrier, bonding, moisturizing, anti-aging and anti-allergic effects. The latest research shows that it also has the function of activating various protein kinases and transcription factors to participate in intracellular signal transduction, thereby affecting various physiological and pathological processes such as cell growth, proliferation, differentiation, apoptosis and injury. As a new type of biologically active substance, ceramide has broad application prospects in the medical care and cosmetic industries. [0003] The ceramides currently used in practice are generally natural extracts, which are obtained from p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C233/20C07C231/22
Inventor 刘铮张敏莲谢建平丁富新袁乃驹
Owner TSINGHUA UNIV
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