Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes
A functional, traditional Chinese medicine fish technology, applied in the field of feed, can solve problems such as prevention and no solution, achieve the effects of improving stability and utilization, promoting healthy growth of fish, and improving feeding ability
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[0034] Preparation of reference solution:
[0035] Accurately weigh an appropriate amount of naringin reference substance, add methanol to make a solution with a concentration of 0.1336 mg / mL, ultrasonically treat for 10 min, and filter through a 0.22 μm microporous membrane to obtain the reference substance solution.
[0036] Preparation of the test solution:
[0037] Mix the bran-fried citrus aurantium, the bran-fried Atractylodes Rhizoma and the lotus leaf, add water for reflux extraction, and evaporate the extract to dryness to obtain an extract, add methanol to the volume to 20 mL, ultrasonically treat for 10 min, and filter with 0.22 μm micropores. After membrane filtration, the test solution was obtained.
[0038] Sample determination:
[0039] Precisely draw 10 μL of the reference solution and the test solution, respectively, and inject them into a liquid chromatograph for content determination. For the HPLC chromatograms of the reference substance and the test subs...
Embodiment 1
[0040] Embodiment 1. Exploration on the extraction process of traditional Chinese medicine compound formula for promoting gastrointestinal health
[0041] The formula of Chinese herbal compound extract:
[0042] Bran fried citrus aurantium 15g; bran fried Atractylodes 19g; lotus leaf 8g.
[0043] The above medicinal materials were weighed, and naringin was used as the index to carry out single factor analysis and response surface design of the compound formula, and the preparation of anti-influenza floating fish bait was carried out.
[0044] 1. One-factor experiment
[0045] 1.1 Influence of the amount of water added on the decoction process
[0046] Take 15g bran fried citrus aurantium, 10g bran fried Atractylodes, 8g lotus leaf, a total of 5 parts, put in a round-bottomed flask, add 4, 6, 8, 10, 12 times the amount of water respectively, decocted for 2 times, 1 hour each time, Combine the decoction liquid, centrifuge, take the supernatant, evaporate to dryness, and add m...
Embodiment 2
[0072] Embodiment 2, the preparation of gastrointestinal health-promoting functional traditional Chinese medicine fish bait
[0073] 1. Preparation of functional traditional Chinese medicine compound liquid for promoting gastrointestinal health
[0074] According to the above-mentioned optimal extraction process, the traditional Chinese medicine compound liquid is extracted and concentrated under reduced pressure to a relative density of 1.0, so as to obtain the effective components of the functional traditional Chinese medicine for promoting gastrointestinal health.
[0075] 2. Dosage and adding method of traditional Chinese medicine ingredients
[0076] Dosage of traditional Chinese medicine ingredients: add according to the mass ratio of the active ingredient extract of traditional Chinese medicine accounting for 2% of the whole fish bait. Therefore, 1Kg of fish bait was prepared, and 20mL of the effective extract of traditional Chinese medicine was added with a concentrat...
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