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Preparation method and application of acellular porcine dermal matrix antibacterial conductive skin scaffold

A technology of decellularized porcine dermis and decellularized dermis, which is applied in the fields of medical science and prostheses, can solve the problems of poor antibacterial effect and low biocompatibility, so as to promote the regeneration of cells and blood vessels, promote cell growth, The effect of accelerating angiogenesis

Active Publication Date: 2022-07-12
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide the preparation method of decellularized porcine dermal matrix antibacterial conductive skin scaffold, which can solve the problem of poor antibacterial effect and low biocompatibility of existing skin scaffolds The problem

Method used

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  • Preparation method and application of acellular porcine dermal matrix antibacterial conductive skin scaffold
  • Preparation method and application of acellular porcine dermal matrix antibacterial conductive skin scaffold
  • Preparation method and application of acellular porcine dermal matrix antibacterial conductive skin scaffold

Examples

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Embodiment 1

[0048] 1) Preparation of micron-sized porous copper oxide microspheres: Accurately weigh 2.5 g of copper nitrate hydrate Cu(NO 3 ) 2 ·3H 2 O was mixed with 20 mL of ethanol, 20 mL of ammonium hydroxide aqueous solution and 10 mL of aqueous sodium hydroxide solution, and the mixture was transferred to a hydrothermal reactor and heated at 140 °C for 14 hours. The precipitate was collected by centrifugation and washed alternately with deionized water and absolute ethanol. Three times, dried in an oven at 50 °C for 8 h to obtain micron-sized porous copper oxide microspheres.

[0049] 2) Preparation of pH-controlled release acellular porcine dermal matrix antibacterial conductive skin scaffold: Take 0.5 mL of multi-walled carbon nanotube aqueous solution and 0.05 mL of micron-sized porous copper oxide microsphere aqueous solution in 5.0 mL of deionized water for 10 minutes ultrasonic treatment, cut The porcine acellular dermal matrix with a diameter of 3.0 cm was immersed in the ...

Embodiment 2

[0051] 1) Preparation of micron-sized porous copper oxide microspheres: Accurately weigh 3.0 g of copper nitrate hydrate Cu(NO 3 ) 2 ·3H 2 O was mixed with 30 mL of ethanol, 30 mL of ammonium hydroxide aqueous solution and 20 mL of sodium hydroxide, and the mixture was transferred to a hydrothermal reactor and heated at 120 °C for 12 hours. The precipitate was collected by centrifugation and washed three times alternately with deionized water and absolute ethanol. , and dried in an oven at 50 °C for 8 h to obtain micron-sized porous copper oxide microspheres.

[0052] 2) Preparation of pH-controlled release decellularized porcine dermal matrix antibacterial conductive skin scaffold: Take 0.1 mL of multi-walled carbon nanotubes aqueous solution and 0.1 mL of micron-sized porous copper oxide microsphere aqueous solution in 7.5 mL of deionized water for 20 minutes of ultrasonic treatment, cut out The pADM with a diameter of 3.0 cm was immersed in the mixed solution and continuo...

Embodiment 3

[0054] 1) Preparation of micron-sized porous copper oxide microspheres: Accurately weigh 5.0 g of copper nitrate hydrate Cu(NO 3 ) 2 ·3H 2 O was mixed with 40 mL of ethanol, 40 mL of ammonium hydroxide aqueous solution and 30 mL of sodium hydroxide, and the mixture was transferred to a hydrothermal reactor and heated at 100 °C for 6 hours. The precipitate was collected by centrifugation and washed three times alternately with deionized water and absolute ethanol. , and dried in an oven at 50 °C for 6 h to obtain micron-sized porous copper oxide microspheres.

[0055] 2) Preparation of pH-controlled release acellular porcine dermal matrix antibacterial conductive skin scaffold: Take 2.0 mL of multi-walled carbon nanotubes aqueous solution and 0.2 mL of micron-sized porous copper oxide microspheres aqueous solution in 10.0 mL of deionized water for 10 minutes ultrasonic treatment, cut out The pADM with a diameter of 3.0 cm was immersed in the mixed solution and continuously sh...

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Abstract

The invention discloses a preparation method of an acellular porcine dermal matrix antibacterial conductive skin scaffold, which specifically comprises the following steps: preparing micron-sized porous copper oxide microspheres by a hydrothermal method, ultrasonically dispersing the micron-sized porous copper oxide microspheres and a multiwalled carbon nanotube aqueous solution uniformly, and loading into a porcine acellular dermal matrix, adding polyethylene glycol diacrylate as a covering layer, and freeze-drying the porcine acellular dermal matrix to obtain the antibacterial conductive skin scaffold. The porcine acellular dermal matrix / micron-sized porous copper oxide microsphere / carbon nanotube antibacterial conductive skin scaffold prepared by the invention has the multifunctional characteristics of antibacterial property, conductivity, tissue regeneration induction capability and the like, can realize real-time monitoring on sports health, and can be applied to wound repair and missing skin tissue reconstruction; and the integration of exercise health monitoring and tissue repair is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedical material preparation, and in particular relates to a preparation method of an acellular pig dermal matrix antibacterial conductive skin support, and also relates to the application of the support. Background technique [0002] The skin is the largest organ of the human body. Acute wounds such as surgical incisions and chronic wounds such as ulcers will cause damage or even loss of the skin, and the wounds are susceptible to bacterial infection and cause greater harm to the human body. The use of antibiotics in wounds will be accompanied by the emergence of drug resistance, and the use of bacteriostatic substances such as quaternary ammonium salts and metallic silver will bring certain cytotoxicity. Since bacterial metabolism in wounds will make the physiological environment acidic, it is necessary to develop a medical skin scaffold that can control the release of antibacterial substances under ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60A61L27/56A61L27/02A61L27/08A61L27/50A61L27/54
CPCA61L27/3633A61L27/60A61L27/56A61L27/025A61L27/08A61L27/50A61L27/54A61L2300/102A61L2300/404A61L2400/12A61L27/362A61L27/36A61L27/40
Inventor 刘新华崔博强郑漫辉白忠薛费一凡王学川姜慧娥
Owner SHAANXI UNIV OF SCI & TECH
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