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Strain with iron reduction capability and electrochemical activity and application thereof

An electrochemical and capable technology, applied in biochemical equipment and methods, bacteria, biochemical fuel cells, etc., to achieve wide application prospects and good power generation effects

Active Publication Date: 2022-07-05
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the pure culture diversity of Shewanella in the mangrove ecosystem, and only one humus-reducing bacterium, Shewanella, was found by Wu Peng et al. sp.W3 and a new species Shewanella mangrovi isolated by Liu et al. (2015)

Method used

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  • Strain with iron reduction capability and electrochemical activity and application thereof
  • Strain with iron reduction capability and electrochemical activity and application thereof
  • Strain with iron reduction capability and electrochemical activity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1: Isolation and identification of Shewanella sp. C31

[0038] 1. Preparation of culture medium

[0039] LB liquid medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH=7.4. Solid medium was prepared by adding 2% (w / v) agar powder to the above fresh medium.

[0040] 2. Isolation of strains

[0041] Weigh 2 g of mangrove sediment samples collected in the Mangrove Ecological Reserve (22°30′-22°32′N, 113°56′-114°3′E) in Futian District, Shenzhen City, Guangdong Province, and mix it with 50mL sterile Mix the water well, stand still for precipitation, take the supernatant with 0.9% normal saline and press 10 -1 ~10 -5 serial dilution, the 10 -3 Diluted supernatant, 10 times -4 Diluted supernatant, 10 times -5 The double-diluted supernatant was evenly spread on the LB medium plate with a coating rod, and cultured in a constant temperature incubator at 30 °C for one week. Overlined for further purification. After culturing the strain on LB medi...

Embodiment 2

[0062] Example 2: Iron reducing ability of Shewanella sp. C31

[0063] The strain Shewanella sp. C31 was cultured on a LB liquid medium in a constant temperature culture shaker (180 rpm, 30° C.) to logarithmic growth phase (OD 600 =0.8~1.0), high-speed centrifugation (5000×g, 15min) to harvest bacteria, resuspend and centrifuge with 1×PBS buffer to remove impurities in LB medium, wash three times, and then resuspend to OD with 1×PBS buffer 600 =0.8~1.0, the obtained bacterial liquid is filled with N 2 Deoxygenation, aerated for about 20 min, and ferric citrate (56 mM) basal medium and ferrihydrite (100 mM) medium supplemented with 20 mM sodium lactate (electron donor) were added according to 10% (v / v) inoculum volume. The two mediums were set up in three parallels, placed in a 30°C constant temperature incubator to avoid light, and sampled at regular intervals. Ferrozine method was used to monitor the iron reduction of strain Shewanella sp. C31. .

[0064] Wherein, the prep...

Embodiment 3

[0096] Example 3: Electricity generation effect of Shewanella sp. C31

[0097] A single-chamber reactor bioelectrochemical system (BES) was constructed, which consisted of a 100 mL multi-port reactor, working electrode (graphite plate), counter electrode and reference electrode (saturated calomel electrode). Both the working electrode and the counter electrode are graphite plates with an area of ​​4.5cm 2 (3cm×1.5cm), the thickness is 0.5cm. Assemble 3 identical cell reactors as parallels. 80mL of electrolyte was added to the reactor, and the mixed gas (CO 2 :N 2 =20:80) After deoxygenation and high temperature sterilization, the strain Shewanella sp. C31 grown to the logarithmic growth phase was inoculated into the reactor at an inoculation amount of 10% (v / v). Then connect the electrochemical workstation to the single-chamber reactor and computer, monitor and collect current data in real time in i-t mode, and judge the remaining status of electron donors according to the...

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Abstract

The invention provides a bacterial strain with iron reduction capacity and electrochemical activity and application thereof, and belongs to the technical field of microbial system classification and environmental biology. The bacterial strain with the iron reducing capacity and the electrochemical activity is named shewanella sp. C31, is preserved in Guangdong Microbial Culture Collection Center (GDMCC) of Institute of Microbiology, Guangdong Academy of Sciences, located on the fifth floor, No. 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City on January 17, 2022, and has the preservation number of CDMCCNO: 62214. The strain with the iron reducing capacity and the electrochemical activity can grow in an anaerobic environment by using ferric citrate or ferrihydrite as an electron acceptor and sodium lactate as a unique electron donor, and has a relatively good electrogenesis effect in a bioelectrochemical system; the method has a wide application prospect in the aspects of environmental pollution repair and microbial electrochemical research.

Description

technical field [0001] The invention belongs to the field of microbial system classification and environmental biotechnology, relates to a bacterial strain with iron reducing ability and electrochemical activity and an application thereof, in particular to a Shewanella sp. ) C31 and its applications. Background technique [0002] Mangroves are a special type of ecosystem that is an intertidal transition zone between terrestrial and marine habitats. Environmental factors such as salinity and nutrient availability in this ecosystem are highly variable, and the environment is aerobic / anaerobic, which determines the rich biodiversity. These microbes living in mangroves play an important role in nutrient transformation and ecological functions. They are known as "coast guards" and "green lungs of the ocean", and are also important habitats for rare and endangered waterfowl. [0003] Shewanella is a facultative anaerobe with extracellular respiration. It is widely distributed i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00H01M8/16C22B1/00C12R1/01
CPCC12N1/20C12P3/00H01M8/16C22B1/00Y02E60/50
Inventor 杨贵芹姚思婕庄莉张雪莹
Owner JINAN UNIVERSITY
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