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Chitosan nanoparticles with high stability and high biological safety as well as preparation method and application of chitosan nanoparticles

A biosafety, chitosan nanotechnology, applied in the field of nanomaterials and biology, can solve the problems of poor water solubility of photosensitizers and weak cell interaction, and achieve strong bactericidal effect and high anti-microbial activity.

Pending Publication Date: 2022-05-13
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Purpose of the invention: The purpose of the present invention is to provide a highly stable and high biosafety chitosan nanoparticle to solve the problems of poor water solubility and weak cell interaction of photosensitizers, improve photodynamic antibacterial activity, and develop efficient, safe and stable The broad-spectrum PDT antimicrobial drug is used to cope with the lack of antimicrobial drug caused by the increasingly severe microbial drug resistance; the present invention also provides the preparation and application of this nanoparticle

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  • Chitosan nanoparticles with high stability and high biological safety as well as preparation method and application of chitosan nanoparticles
  • Chitosan nanoparticles with high stability and high biological safety as well as preparation method and application of chitosan nanoparticles
  • Chitosan nanoparticles with high stability and high biological safety as well as preparation method and application of chitosan nanoparticles

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Embodiment 1

[0035] Embodiment 1 Preparation of CMCS-PEI-PpIX nanoparticles

[0036] Dissolve 2 g of PEI and 10 mg of PpIX in 10 mL of ultrapure water. The solution was transferred to a 25mL hydrothermal reactor and reacted at 120°C for 8h. After cooling to room temperature, it was dialyzed with ultrapure water with a molecular weight of 1kDa for 1d, and passed through a 0.45μm filter membrane to obtain PEI-PpIX.

[0037] Add 5 mg CMCS and 50 µL EDC to 5 mL of freshly prepared PEI-PpIX solution. The mixture was magnetically stirred at 35 °C for 16 h. After the reaction, the CMCS-PEI-PpIX nanoparticles were obtained by dialysis with deionized water (MWCO=10kDa) for 1 d and passing through a 0.45 μm filter membrane.

Embodiment 2

[0038] Scanning electron microscope observation of embodiment 2 CMCS-PEI-PpIX nanoparticles:

[0039] The filtered CMCS-PEI-PpIX nanoparticles were diluted with deionized water, 10 μL was dropped on a clean silicon wafer, and observed with a scanning electron microscope (Ultra Plus; Zeiss Ltd., Germany), as shown in figure 1 shown. The result of transmission electron microscope observation shows that the nanoparticles have a nearly spherical structure and are evenly distributed.

Embodiment 3

[0040] Example 3 CMCS-PEI-PpIX Nanoparticle Size Distribution Detection

[0041] measure by scale figure 1 The diameter of the nanoparticle obtained by scanning electron microscope observation, and its particle size distribution are counted, such as figure 2 As shown, it shows that the average particle diameter of the nanoparticles is about 300nm;

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Abstract

The invention provides a preparation method of a chitosan nano material (CMCS-PEI-PpIX nano particle) with high stability and high biological safety and an application of the chitosan nano material in broad-spectrum photodynamic microbe resistance. The preparation method comprises the following steps: taking protoporphyrin (PpIX) and ethyleneimine (PEI) as raw materials, and obtaining PEI-PpIX through a one-step hydrothermal method. Then, the PEI-PpIX and carboxymethyl chitosan (CMCS) are used as raw materials, and CMCS-PEI-PpIX with negative charges is generated through EDC / NHS reaction. The CMCS-PEI-PpIX is self-assembled into nano particles in a solution, and shows a remarkably enhanced singlet oxygen yield in the solution. Therefore, the CMCS-PEI-PpIX nanoparticles have very good antibacterial PDT activity on gram-negative bacteria, gram-positive bacteria and fungi, and meanwhile, the CMCS-PEI-PpIX nanoparticles have no toxicity to normal cells and are highly stable. The invention provides an effective way for effectively killing disease microorganisms in food, medical supplies and food and medicine processing environments.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and in particular relates to a highly stable and high biological safety chitosan nanoparticle, a preparation method and an application. Background technique [0002] Photodynamic antimicrobial therapy (Antimicrobial Photodynamic Therapy, PDT) is the use of photosensitizers to generate reactive oxygen species (ROS) under light irradiation, such as singlet oxygen ( 1 o 2 ) and free radicals to kill microorganisms (Chem.Soc.Rev.2010,39,2835-2846). The generated ROS can damage key biomolecules, including lipids (Chem.Sci.2020, 11, 3405-3417), proteins (J.Am.Chem.Soc.2016, 138, 10968-10977) and nucleic acids (J.Biol Chem. 2001, 276, 6056-40604), leading to cell necrosis / apoptosis and tissue damage (Angew. Chem. Int. Ed. Engl. 2019, 58, 2558-2569). PDT is an effective method against microbial infections and is considered as a potential strategy to replace traditional antibiotics to eli...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/59A61K47/61A61K47/69A61P31/04A61P31/10B82Y5/00A01N25/10A01N43/90A01P1/00A01P3/00
CPCA61K47/61A61K47/59A61K47/6939A61K47/6935A61K41/0071A61P31/04A61P31/10B82Y5/00A01N25/10A01N43/90
Inventor 林凤鸣章杰
Owner SOUTHEAST UNIV
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