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Application of hemicyanine small molecule compound as fluorescent probe and photodynamic antibacterial agent

A fluorescent probe and compound technology, applied in the field of biomedicine, can solve problems such as strong toxicity, easy photobleaching, and poor photostability, and achieve the effects of avoiding self-absorption, large displacement, and high yield

Active Publication Date: 2022-03-08
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a series of application research progress has been made in biochemical detection, it still has obvious disadvantages such as strong toxicity, poor photostability, and easy photobleaching. Biocompatibility of Novel Fluorescent Probes as Detection Materials

Method used

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  • Application of hemicyanine small molecule compound as fluorescent probe and photodynamic antibacterial agent
  • Application of hemicyanine small molecule compound as fluorescent probe and photodynamic antibacterial agent
  • Application of hemicyanine small molecule compound as fluorescent probe and photodynamic antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0029] According to the methods disclosed in Document 1, Document 2 and Document 3, semicyanine small molecule compounds are prepared, and its structural formula is as follows:

[0030]

[0031] Wherein, n=3, 6 or 10, the corresponding compounds are respectively counted as C3, C6 and C10;

[0032]Among them, Document 1 is: W.H.Zhao, J.P.Su, H.R.Ye, L.H.Gao, K.Z.Wang, Fourphotoelectroactive composite films based on [BW11O39M(H2O)]7-(M?Zn,Cu) and bipolar hemicyanines, Mater.Res.Bull. 92 (2017) 1–8;

[0033] Document 2 is: B.Q.Song, X.L.Wang, C.Y.Sun, Y.T.Zhang, X.S.Wu, L.Yang, K.Z.Shao, L.Zhao, Z.M.Su, An organic-inorganic hybrid photocatalyst based on sandwich-type tetra-Co-substituted phosphotungstates with high visible lightphotocatalytic activity, Dalton Trans. 44 (2015) 13818–13822;

[0034] Document 3 is: L.H.Gao, W.C.Jiang, K.Z.Wang, Preparation and photocatalytic activity of an electrostatically self-assembled film made of [PMo12O40]3-and abipolar hemicyanine cation...

Embodiment 4

[0036] Using 2,7-dichlorofluorescein diacetate (DCFH-DA) as a detection probe for active oxygen (ROS), test the ability of the semicyanine small molecule compounds prepared in Examples 1 to 3 to sensitize ROS, Specifically include the following steps:

[0037] 4.87 mg of DCFH-DA was dissolved in 1 mL of ethanol to obtain a 10 mM stock solution of DCFH-DA and placed in a -20°C refrigerator for use.

[0038] Before the experiment, 50 μL of 10 mM DCFH-DA stock solution was added to 450 μL of ethanol and 2 mL of 0.01M NaOH aqueous solution, and then stored and activated at room temperature in the dark for 30 minutes to hydrolyze DCFH-DA to obtain 2,7-dichlorodihydrofluorescein (DCFH). Add 10 mL of 1×PBS (10 mM, pH=7.4) to obtain a DCFH solution with a final concentration of 40 μM and store it on ice in the dark.

[0039] In the presence of reactive oxygen species (ROS), DCFH will be converted into 2,7-dichlorofluorescein (DCF, excitation 488nm, emission 524nm) with a high quantum...

Embodiment 5

[0045] In different pathogenic microorganisms: Escherichia coli (Amp r E.coli), Staphylococcus aureus (S.aureus), fungus Candida albicans (C.albicans) by adding the semicyanine small molecule compounds prepared in Examples 1 to 3, using a confocal laser scanning microscope (CLSM) or Fluorescence microscopy is used to detect whether it can stain pathogenic microorganisms, which may specifically include the following steps:

[0046] Take 100μL OD 600 =1.0 was added to the semicyanine C3, C6, and C10 prepared in Examples 1 to 3, and then 1×PBS (10mM, PH=7.4) was added to supplement the total volume to 500 μL, so that the final concentration of the semicyanine C3, C6, and C10 was 0.5 μM and divided into light group and dark group.

[0047] Place the samples in a mold incubator (37°C for bacteria, 30°C for fungi) and incubate for 30min. After taking it out, the light-proof group was wrapped in tin foil and placed in a dark place, while the light-emitting group was exposed to whi...

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PUM

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Abstract

The invention provides application of a compound with a structure as shown in a formula (I) as a fluorescent probe and a photodynamic bactericide and in preparation of a kit for detecting pathogenic microorganisms or a kit for detecting whether escherichia coli survives or not. Experimental results show that the compound with the structure as shown in the formula (I) has excellent capability of sensitizing oxygen to generate ROS; the antibacterial film can be successfully attached to or inserted into an escherichia coli film, provides the possibility of killing pathogenic microorganisms while serving as an imaging material, and has the capacity of distinguishing viable bacteria from dead bacteria; the fluorescent probe can interact with staphylococcus aureus or candida albicans to achieve a marking effect, and is used as a fluorescent probe for detecting pathogenic microorganisms; the compound has broad-spectrum antibacterial activity and efficient photodynamic antibacterial ability.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of a semicyanine small molecule compound as a fluorescent probe and a photodynamic antibacterial agent. Background technique [0002] Photodynamic therapy (PDT) has been proven to be a non-invasive and highly selective therapeutic technique widely used in the fields of sterilization and cancer treatment. PDT generally consists of three parts: light source, photosensitizer and oxygen molecules. Under the irradiation of light source, the photosensitizer absorbs energy to sensitize the surrounding oxygen to generate reactive oxygen species (ROS), such as singlet oxygen ( 1 o 2 ), superoxide anion (·O 2 - ), hydroxyl radicals (OH), etc., which can cause severe oxidative stress and lead to cell death. Compared with traditional therapies (such as surgery, chemotherapy, and radiotherapy), photodynamic therapy has the following advantages: (1) high selectivity for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/38C09B23/14C09K11/06C12Q1/04C12Q1/10C12Q1/14G01N21/64A61K41/00A61P31/04A61P31/10
CPCC07D213/38C09B23/145C09K11/06A61K41/0057A61P31/04A61P31/10G01N21/6458C12Q1/14C12Q1/10C12Q1/04C09K2211/1029Y02A50/30
Inventor 袁焕祥李泽琳高丽华
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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