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Manufacturing method of rat myocardial infarction model

A technology of myocardial infarction and production method, applied in the field of medicine, can solve the problems of large damage, inaccurate embolism target, high mortality rate, etc., and achieve the effect of small damage, exact effect and low mortality rate

Pending Publication Date: 2021-12-31
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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Problems solved by technology

[0005] The purpose of the present invention is to provide a new rat myocardial infarction model production method, to overcome the need to use needles and threads to ligate the local myocardium in the prior art, causing great damage and high mortality; also solves the technical problems of lauric acid The sodium model requires retrograde injection through the aortic arch or the left ventricle, and the embolization target is not exact technical problems

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  • Manufacturing method of rat myocardial infarction model
  • Manufacturing method of rat myocardial infarction model

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Effect test

Embodiment 1

[0023] Embodiment 1 rat myocardial infarction model making method

[0024] 1) Using 10% chloral hydrate, 0.3ml / 100g, intraperitoneal injection for anesthesia, fixed behind the small animal operating table, and then performed tracheal intubation with a small animal laryngoscope, connected to a ventilator to assist breathing, and connected Small animal biological signal acquisition and analysis system;

[0025] 2) Draw 1ml of blood from the tail vein, add 50μl of thrombin freeze-dried powder to promote its coagulation, and then put the coagulated blood into a beaker filled with normal saline, and repeatedly suck it with a syringe to break it into tiny thrombi. Use a 50 μl micro-syringe to aspirate 25 μl of thrombus for use;

[0026] 3) Open the thoracotomy layer by layer from the 3-5 intercostal space of the left sternum of the rat, use 2-3 small cotton balls to insert under the heart to raise the heart, fully expose the heart, select the position of the left main coronary arte...

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Abstract

The invention relates to a manufacturing method and application of a rat myocardial infarction model. The method comprises the steps of anesthesia, intubation, connection with a breathing machine to assist breathing, caudal vein blood drawing, solidification, breaking into tiny thrombus, layer-by-layer thoracotomy of intercostal space, injection of thrombus into a left trunk in the coronary artery direction, and observation of the electrocardiogram condition of a rat. Accurate embolism is caused by using autologous thrombus, target blood vessels can be embolized more accurately, the autologous thrombus can better reflect the pathophysiological state of model I-type myocardial infarction and has no rejection reaction, and a local coronary artery injection technology is far smaller in injury than ligation, more accurate in effect and lower in death rate. The method can be applied to medicines for evaluating and treating myocardial infarction and treatment methods for evaluating and treating myocardial infarction.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for making a rat myocardial infarction model. Background technique [0002] Myocardial infarction refers to the loss of myocardial cells caused by prolonged myocardial ischemia, which is the result of an imbalance between the supply and demand of blood perfusion. Due to acute occlusion of the coronary artery, partial myocardial necrosis occurs due to severe and persistent ischemia. Clinically, it includes persistent retrosternal pain, shock, fever, leukocytosis, increased erythrocyte sedimentation rate, and progressive electrocardiogram ischemia and injury. Sexual changes etc. The disease is prone to occur in the elderly, and the elderly who suffer from hypertension, obesity, diabetes, dyslipidemia, and inactivity are more likely to suffer from the disease. Patients usually have precordial pain for a long time, often half an hour, and the pain cannot be re...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 何尤夫吴强周瑜黄晶龙向淑
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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