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Quality control method of amygdalin in children lung heat cough and asthma granules

A quality control method, a technique for lung-heat, cough and asthma, applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of poor separation of amygdalin peaks, and achieve clear spots, good reproducibility, and good separation effect Effect

Active Publication Date: 2021-12-24
HAINAN HULUWA PHARMA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difference between raw materials and auxiliary materials in the above-mentioned medicine and the present invention, the peak separation effect of amygdalin using the above-mentioned method is poor

Method used

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  • Quality control method of amygdalin in children lung heat cough and asthma granules
  • Quality control method of amygdalin in children lung heat cough and asthma granules
  • Quality control method of amygdalin in children lung heat cough and asthma granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Differentiation test of amygdalin in Xiaoer Feirekechuan Granules

[0051] (1) Prepare 200 mL of developing agent, which is prepared by mixing cyclohexane, chloroform, formic acid and toluene at a volume ratio of 12:0.5:3:7.

[0052] (2) Test solution: take 3 batches of Feire Kechuan granules for children respectively, dissolve them in acetonitrile with a volume fraction of 40%, vortex oscillation speed is 700r / min, oscillation time is 2min, filter with 0.45μm polytetrafluoroethylene Membrane filtration to prepare a test solution with a concentration of 0.1 mg / mL.

[0053] (3) Control solution: Dissolve the amygdalin reference substance in acetonitrile with a volume fraction of 40%, shake well, and filter through a 0.45 μm polytetrafluoroethylene filter membrane to prepare a control solution with a concentration of 0.1 mg / mL.

[0054](4) Take 7 μl each of test solution 1, test solution 2, test solution 3, and control solution, respectively, and place them on ...

Embodiment 2

[0056] Determination of the content of amygdalin in Example 2 Xiaoerfeirekechuan Granules

[0057] Chromatographic conditions

[0058] Mobile phase A: the mobile phase A is 0.3% lithium bromide, 15% acetone and the rest of acetonitrile by mass percentage.

[0059] Mobile phase B: use N,N-dimethylformamide aqueous solution with a mass concentration of 0.3% as mobile phase B;

[0060] Chromatographic column: the stationary phase is Boston Green ODS chromatographic column (4.6×250mm, 5um).

[0061] Detection wavelength: 210nm.

[0062] Injection volume: 20 μl.

[0063] Column temperature: 30°C.

[0064] The elution conditions are:

[0065]

[0066]

Embodiment 3

[0067] Determination of the content of amygdalin in Example 3 Xiaoerfeirekechuan Granules

[0068] Chromatographic conditions

[0069] Mobile phase A: 0.1% lithium bromide by mass percentage, 10% acetone and acetonitrile as the mobile phase A.

[0070] Mobile phase B: use N,N-dimethylformamide aqueous solution with a mass concentration of 0.2% as mobile phase B.

[0071] Chromatographic column: Boston Green ODS (4.6×250mm, 5um).

[0072] Detection wavelength: 210nm.

[0073] Injection volume: 20 μl.

[0074] Column temperature: 30°C.

[0075] The elution conditions are:

[0076] time (min) Mobile phase A (v / v) Mobile phase B (v / v) 0～15.0 5 95 15.01～25.0 20 80 25.01～40.0 42 58 40.01～70.0 5 95

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Abstract

The invention provides a quality control method of amygdalin in children lung heat cough and asthma granules. A thin-layer chromatography identification method has the advantages of small sample application amount, good separation effect, clear spots, moderate specific shift value and good reproducibility. Quantitative analysis is carried out on the amygdalin component in the infantile lung-heat cough and asthma granules through content determination, it is verified that the content of the amygdalin in the infantile lung-heat cough and asthma granules can be accurately and efficiently detected through content determination chromatographic conditions, and through combination of a thin-layer chromatography identification method and content, the content of amygdalin in the children lung heat cough and asthma granules can be effectively controlled, and the medication safety of the children lung heat cough and asthma granules is ensured.

Description

technical field [0001] The invention relates to the field of amygdalin detection, in particular to a method for quality control of amygdalin in Xiaoerfeirekechuan granules. Background technique [0002] Bitter almonds are the dried and mature seeds of the Rosaceae plant Apricot apricot, Siberia apricot, Northeast apricot or apricot. Bitter in nature and flavor, tepor, slightly poisonous; returns lung, large intestine meridian. It has the effects of reducing qi, relieving cough and asthma, moistening the intestines and laxative, and is mostly used for cough and asthma, full chest phlegm, intestinal dryness and constipation. [0003] Amygdalin is a common cyanogenic glycoside and an active ingredient in the traditional Chinese medicine bitter almond. So far, it has become a commonly used expectorant and antitussive agent in medicine, as well as an auxiliary anticancer drug. In addition to amygdalin, bitter almonds generally contain β-glucosidase. Laetrile can generate gluco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 刘景萍刘全国陈克领林海银吴育强
Owner HAINAN HULUWA PHARMA GRP CO LTD
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