Quality control method of amygdalin in children lung heat cough and asthma granules
A quality control method, a technique for lung-heat, cough and asthma, applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of poor separation of amygdalin peaks, and achieve clear spots, good reproducibility, and good separation effect Effect
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Embodiment 1
[0050] Example 1 Differentiation test of amygdalin in Xiaoer Feirekechuan Granules
[0051] (1) Prepare 200 mL of developing agent, which is prepared by mixing cyclohexane, chloroform, formic acid and toluene at a volume ratio of 12:0.5:3:7.
[0052] (2) Test solution: take 3 batches of Feire Kechuan granules for children respectively, dissolve them in acetonitrile with a volume fraction of 40%, vortex oscillation speed is 700r / min, oscillation time is 2min, filter with 0.45μm polytetrafluoroethylene Membrane filtration to prepare a test solution with a concentration of 0.1 mg / mL.
[0053] (3) Control solution: Dissolve the amygdalin reference substance in acetonitrile with a volume fraction of 40%, shake well, and filter through a 0.45 μm polytetrafluoroethylene filter membrane to prepare a control solution with a concentration of 0.1 mg / mL.
[0054](4) Take 7 μl each of test solution 1, test solution 2, test solution 3, and control solution, respectively, and place them on ...
Embodiment 2
[0056] Determination of the content of amygdalin in Example 2 Xiaoerfeirekechuan Granules
[0057] Chromatographic conditions
[0058] Mobile phase A: the mobile phase A is 0.3% lithium bromide, 15% acetone and the rest of acetonitrile by mass percentage.
[0059] Mobile phase B: use N,N-dimethylformamide aqueous solution with a mass concentration of 0.3% as mobile phase B;
[0060] Chromatographic column: the stationary phase is Boston Green ODS chromatographic column (4.6×250mm, 5um).
[0061] Detection wavelength: 210nm.
[0062] Injection volume: 20 μl.
[0063] Column temperature: 30°C.
[0064] The elution conditions are:
[0065]
[0066]
Embodiment 3
[0067] Determination of the content of amygdalin in Example 3 Xiaoerfeirekechuan Granules
[0068] Chromatographic conditions
[0069] Mobile phase A: 0.1% lithium bromide by mass percentage, 10% acetone and acetonitrile as the mobile phase A.
[0070] Mobile phase B: use N,N-dimethylformamide aqueous solution with a mass concentration of 0.2% as mobile phase B.
[0071] Chromatographic column: Boston Green ODS (4.6×250mm, 5um).
[0072] Detection wavelength: 210nm.
[0073] Injection volume: 20 μl.
[0074] Column temperature: 30°C.
[0075] The elution conditions are:
[0076] time (min) Mobile phase A (v / v) Mobile phase B (v / v) 0~15.0 5 95 15.01~25.0 20 80 25.01~40.0 42 58 40.01~70.0 5 95
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