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Fermentation method for increasing yield of riboflavin

A fermentation method and a riboflavin technology are applied in the field of fermentation for increasing the yield of riboflavin, which can solve the problems of long fermentation period of riboflavin, waste of resources and low utilization rate of nutrients, etc., and achieves optimized absorption, increased yield, maintained The effect of growth vitality

Pending Publication Date: 2021-12-07
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, riboflavin production by fermentation has a long fermentation period, low yield and low nutrient utilization rate, resulting in serious waste of resources and increased cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A fermentation method for improving riboflavin production, the specific steps are as follows:

[0027] (1) Activation of strains: the riboflavin-producing Bacillus subtilis was taken out from the -80°C refrigerator, and activated on slant medium for two generations. The composition and content of the slant medium are: tryptone 10g / L, beef heart extract powder 17.5g / L, sodium chloride 5g / L, glucose 2g / L, disodium hydrogen phosphate 2.5g / L, agar powder 25g / L, pH=7.0.

[0028] (2) Seed cultivation: the activated bacteria are eluted with sterile water, and then inserted into the seed medium for cultivation. The composition and content of the seed medium are: grape 80g / L, yeast powder 8g / L, peptone 3g / L, magnesium sulfate heptahydrate 0.5g / L, ammonium sulfate 8g / L, FeSO 4 ·7H 2 O 10mg / L, MnSO 4 ·H 2 O 5mg / L, V H 1mg / L, antifoaming agent 1g / L, and adjust the pH of the medium to 7.0-7.2 with ammonia water.

[0029] (3) Fermentation culture: inoculate 30% of the seed sol...

Embodiment 2

[0031] A kind of fermentation method that improves riboflavin output, with reference to embodiment 1, difference is: do not feed fermentation nutrient, add gluconic acid metal complex in fermentation medium simultaneously (fermentation medium volume is 2L, glucose Acid metal complex is added according to the volume of fermentation medium), its composition and content are: ferrous gluconate, zinc gluconate, calcium gluconate composition, its content is: 100mg / L, 70mg / L, 70mg / L L. When put in the tank for 50 hours, the riboflavin output was 26.7g / L.

Embodiment 3

[0033] A kind of fermentation method that improves riboflavin output, with reference to embodiment 1, difference is: when thalline amount reaches 15, start to add fermentation nutrient (feeding dosage 300ml, use peristaltic pump to feed at a constant speed during fermentation, Flow rate 0.2g / L / h), do not add gluconate metal complex in the fermentation medium. Fermentation nutrient composition and content are: citric acid 3g / L, glutamine 1g / L and KH 2 PO 4 3g / L. When put in the tank for 50 hours, the riboflavin output was 27.3g / L.

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Abstract

The present invention provides a fermentation method for increasing the yield of riboflavin, the gluconic acid metal complex is added into the fermentation culture medium, fermentation nutrients are fed, the functions of carbohydrates can be diversified, absorption of thalli to metal elements is optimized, and the toxic and side effects of the metal elements to the thalli are reduced; along with the proceeding of fermentation, the nutritional ingredients in the fermentation tank are gradually reduced, the vitality of thalli is gradually weakened, and fed-batch fermentation nutrients can supplement the nutritional ingredients required by the thalli in the fermentation process and maintain the growth vitality of the thalli; due to the addition of citric acid, the EMP pathway is inhibited, and the HMP pathway is enhanced; glutamine enhances the generation of riboflavin precursor guanosine triphosphate, and is beneficial to the accumulation of riboflavin; and the bacillus subtilis is used as a growth coupling type strain for producing riboflavin, the quantity of thalli influences the yield of riboflavin to a great extent, and the fed-batch of KH2PO4 can keep the thalli in a good growth state, so that the yield of riboflavin is increased.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a fermentation method for increasing riboflavin production. Background technique [0002] Riboflavin (Riboflavin) is also known as vitamin B2 (VitaminB2), the chemical formula is C 17 h 20 N 4 o 6 , Slightly soluble in water, stable under neutral and acidic conditions, easily soluble in alkali, and irreversibly decomposed when exposed to light under alkaline conditions. Riboflavin is one of the 13 kinds of vitamins necessary for the human body, and it is an essential nutrient for maintaining the normal substance metabolism of human and animal bodies. Because animals and humans lack endogenous riboflavin production pathways, they cannot synthesize it by themselves, and need to supplement an appropriate amount of riboflavin from the outside world. It is called one of the six major indicators of human growth and nutritional status listed by the World Health Orga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P25/00C12N1/20C12R1/125
CPCC12P25/00C12N1/20
Inventor 徐庆阳李澜潇
Owner TIANJIN UNIV OF SCI & TECH
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