Structure and function bionic urethral stent and preparation method thereof

Abstract

The invention provides a structure and function bionic urethral stent and a preparation method thereof. The preparation method comprises the following steps of: (1) uniformly mixing an oxidized bacterial cellulose nanofiber dispersion liquid and a decellularized matrix solution, pouring the mixture into a mold I until the mold I is completely filled, and performing freeze drying to obtain an uncrosslinked porous stent; (2) putting the uncrosslinked porous stent into a cross-linking agent solution, performing cross-linking, rinsing the cross-linked porous stent with water, and performing freeze drying to obtain a cross-linked porous stent; and (3) pouring a mixed solution II consisting of oxidized bacterial cellulose nanofibers, a modified or unmodified natural polymer material, a gelling aid and water into a silica gel mold II until the silica gel mold II is completely filled, fixing the cross-linked porous stent on the mold II, enabling the cross-linked porous stent to be in contact with the mixed solution II, and standing for a period of time to obtain the structure and function bionic urethral stent. The prepared stent consists of a hydrogel layer of a bionic urethral mucosa and a porous layer of a bionic urethral cavernous body, can be rapidly epithelized and vascularized after being implanted into a body, and has a good urethral defect repair effect.

Description

technical field [0001] The invention belongs to the technical field of urethral stents, and relates to a urethral repair stent and a preparation method thereof, in particular to a structural and functional bionic urethral stent and a preparation method thereof. Background technique [0002] Urethral stricture is a urological disease caused by trauma, infection, iatrogenic operation, etc. The global incidence of urethral stricture is 0.3%, especially complex long-segment urethral stricture, which seriously affects the quality of life of patients. Its repair and reconstruction have always been A major problem facing urology clinical practice. Autologous tissue transplantation, such as oral mucosa, penile flap, bladder mucosa, is currently the main measure for the clinical treatment of urethral stricture. However, this method has the limitations of "at the expense of normal tissue" and "limited tissue sources for transplantation". In recent years, the development of tissue en...

AI Technical Summary

Problems solved by technology

The Chinese invention patent with the publication number CN110960727A "a tissue-engineered urethral stent graft and its preparation method and application" discloses a degradable nanofibrous tube of polymer material inoculated with epithelial cells and smooth muscle cells as a tissue-engineered urethra For grafts, epithelial cells are used as seed cells to promote the rapid formation of epithelial layers after graft implantation. However, the source of epithelial cells is limited, the culture process is cumbersome, and sequelae will appear at the site of sampling.