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Ultralow-concentration single-component polypeptide hydrogel as well as preparation method and application thereof

An ultra-low concentration, hydrogel technology, applied in the fields of application, medical science, surgery, etc., can solve the problems of lack of tissue interface clearance, difficult application, long hemostasis time, etc., and achieve high practical application value and excellent biocompatibility Sexuality, the effect of suitable adhesion

Active Publication Date: 2021-11-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The hydrogel has no small molecule residue inside, has self-healing properties, injectability, suitable adhesion, tissue interface clearance, biocompatibility and biodegradability, which not only solves the complicated preparation process of the existing hydrogel , high gelation concentration, difficult application of double (multiple) components, lack of tissue interface clearance, resulting in clinical secondary damage; and significantly overcome the long hemostasis time and hemostasis existing in the application of current wound hemostatic agents, sealants, and trauma accessories. Low rate and poor wound healing effect

Method used

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  • Ultralow-concentration single-component polypeptide hydrogel as well as preparation method and application thereof
  • Ultralow-concentration single-component polypeptide hydrogel as well as preparation method and application thereof
  • Ultralow-concentration single-component polypeptide hydrogel as well as preparation method and application thereof

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preparation example Construction

[0055] The present invention also provides a method for preparing an ultra-low concentration single-component polypeptide hydrogel, comprising the following steps:

[0056] (1) Using carboxylated polypeptide as the polymer skeleton, the catechol-functionalized polypeptide is prepared after catechol modification;

[0057] (2) oxidizing the catechol-functionalized polypeptide obtained in step (1) in an alkaline solution, and then dialyzing or stirring with deionized water;

[0058] (3) Freeze the solution obtained in step (2), thaw at room temperature to obtain an ultra-low concentration single-component polypeptide hydrogel or freeze-dry the solution obtained in step (2) to obtain a solid, and then The polypeptide hydrogel is obtained after the solution is dissolved, and the gelation time is 20-120s.

[0059] In one embodiment of the present invention, the catechol is dopamine or 3,4-dihydroxybenzylamine, etc., and the grafting rate is 10%-50%.

[0060] In one embodiment of t...

Embodiment 1

[0073] This example provides a method for preparing an ultra-low concentration single-component polypeptide hydrogel.

[0074] (1) Dissolve 129 mg (1 mmol) of α-polyglutamic acid (molecular weight 38.7 kDa) with a degree of polymerization of 300 in DMSO (dimethyl sulfoxide), and add 46 mg of 1-(3-dimethylaminopropyl) at low temperature base)-3-ethylcarbodiimide hydrochloride (EDC) (0.24mmol) and 27.6mg N-hydroxysuccinimide (NHS) (0.24mmol) activated overnight, under nitrogen atmosphere, add dopamine hydrochloride 38mg (0.2mmol) and a small amount of triethylamine (TEA) as an acid-binding agent, and reacted at 25°C for 48h, and then the reaction solution was directly dialyzed in PBS with pH=7.4 for 1d, then dialyzed with deionized water for 1d, and then deionized After water dialysis for 1 day, after using ultraviolet light to detect the absence of dopamine (DA) in water, the obtained colorless transparent solution was collected and freeze-dried to obtain a white solid, that is...

Embodiment 2

[0079] This example provides a method for preparing a hybrid polypeptide hydrogel.

[0080] (1) Dissolve 129 mg (1 mmol) of α-polyglutamic acid with a degree of polymerization of 300 in DMSO, add 46 mg EDC (0.24 mmol) and 27.6 mg NHS (0.24 mmol) to activate overnight at low temperature, and add dopamine salt under nitrogen atmosphere Salt 38mg (0.2mmol) and a small amount of TEA were used as acid-binding agent, and after reacting at 25°C for 48h, the reaction solution was directly dialyzed with deionized water, and the resulting solution was collected after using ultraviolet light to detect the absence of DA in the water.

[0081] The schematic diagram of catechol functionalized α-polyglutamic acid H NMR spectrum obtained in this example is as follows figure 1 shown.

[0082] (2) The solution obtained in (1) was dissolved in 0.05M NaHCO 3 Dialyze in the solution for 24 hours at a temperature of 25°C, then dialyze in deionized water for 6 hours to remove NaHCO 3 .

[0083] ...

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Abstract

The invention relates to the field of biomedical materials, in particular to ultralow-concentration single-component polypeptide hydrogel as well as a preparation method and application thereof. Carboxylated polypeptide used as a polymer skeleton is modified in one step to obtain catechol functionalized polypeptide, and then chemical bond-hydrogen bond co-crosslinked single-component polypeptide hydrogel is obtained through oxidation and freezing physical treatment. The ultralow-concentration single-component polypeptide hydrogel not only has ultralow critical gelling concentration and heat-resistant, salt-resistant and urea-resistant stability, but also has tissue interface clearing performance. The ultralow-concentration single-component polypeptide hydrogel has excellent biocompatibility and biodegradability, and can quickly and efficiently stop bleeding and promote wound healing. The single-component polypeptide hydrogel disclosed by the invention is simple in preparation method, low in cost and convenient to use, and has an important clinical application potential in the aspects of wound haemostat, sealant and wound auxiliary materials.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to an ultra-low concentration single-component polypeptide hydrogel and its preparation method and application. Background technique [0002] Worldwide, trauma is the third leading cause of death of all age groups, so there is a lot of research on hemostatic and wound healing dressings, and the market demand is still growing rapidly. Polypeptide and protein hydrogels have been extensively studied in wound hemostatic agents, sealants, and wound accessories, but only a small number of products have entered clinical applications. This is mainly due to the fact that they are mostly multi-component materials, used at a higher gel concentration (5-20wt%), complicated to prepare, difficult to remove from the tissue interface and cause secondary damage, and the immunogenicity and biological properties of protein materials. security etc. For example, the Chinese invention patent (CN 107...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/48C08J3/075C08L77/04C08K3/04A61L24/00A61L24/02A61L24/04A61L26/00
CPCC08G69/48A61L26/008A61L26/0019A61L26/009A61L26/0004A61L24/0042A61L24/0031A61L24/046A61L24/02C08J2377/04C08K3/042C08L77/04
Inventor 柏谦董常明
Owner SHANGHAI JIAO TONG UNIV
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