Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dendritic cell-targeted hybrid dendrimeter/YTHDF1 siRNA compound as well as preparation and application thereof

A technology of dendrimers and amine dendrimers, which is applied in the field of hybrid dendrimers/YTHDF1siRNA complexes and their preparation, can solve undiscovered problems and achieve easy implementation, enhanced tumor immunotherapy, and response conditions mild effect

Active Publication Date: 2021-11-19
DONGHUA UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the results of domestic and foreign literature searches, there is no discovery about the preparation of dendritic cell-targeted dendritic macromolecule-encapsulated gold nanoparticles / YTHDF1 siRNA complex and its application in silencing YTHDF1 gene of dendritic cells and its immunotherapy. Related reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dendritic cell-targeted hybrid dendrimeter/YTHDF1 siRNA compound as well as preparation and application thereof
  • Dendritic cell-targeted hybrid dendrimeter/YTHDF1 siRNA compound as well as preparation and application thereof
  • Dendritic cell-targeted hybrid dendrimeter/YTHDF1 siRNA compound as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Weigh 10 mg of the fifth generation PAMAM dendrimer (G5.NH 2 ) (purchased from Dendritech, USA) and 0.94mg of 1,3-PS (purchased from Shanghai Bailingwei Technology Co., Ltd.), were dissolved in 5mL ultrapure water and 2mL ultrapure water, respectively, and stirred in a water bath at 30°C for 24 hours. After the reaction, use a cellulose dialysis bag with a molecular weight cut-off of 8000-14000 to dialyze in pure water (2L) for 3 days (change the water 3-4 times a day) and freeze-dry to obtain G5.NH 2 -PS;

[0088] (2) Weigh 10mg G5.NH 2 -PS, dissolved in 5 mL of ultrapure water. Subsequently, add 121 μL of HAuCl 4 4H 2 O (purchased from Sinopharm Chemical Reagent Co., Ltd.) aqueous solution (30mg / mL), after stirring for 15 minutes, quickly add 2mL containing 1.67mg NaBH 4 ice solution, reacted for 3 hours. Then use a cellulose dialysis bag with a molecular weight cut-off of 8000-14000 to dialyze in pure water (2L) for 3 days (change the water 3-4 times per da...

Embodiment 2

[0094] For the G5.NH prepared in embodiment 1 step (1) and step (3) 2 Weigh 5mg each of -PS and G5-Man-PS and dissolve in 500μL D 2 In O, carry out nuclear magnetic spectrum analysis (such as figure 2 shown). Such as figure 2 As shown in A, 2.2-3.4ppm is the characteristic peak of the fifth-generation dendrimer, and 1.93ppm is the characteristic peak of 1,3-PS. Through integral calculation, it is obtained that each dendrimer is connected with 19.8 1 ,3-PS. Such as figure 2 As shown in B, the characteristic peak at 8.05ppm is the Schiff base, the characteristic peak at 2.2-3.4ppm is the characteristic peak of the fifth generation dendrimer, and the characteristic peak at 1.93ppm is the characteristic peak of 1,3-PS, and each 6 Man and 20.1 1,3-PS were linked to each dendrimer.

Embodiment 3

[0096] The DNP and MDNP prepared in step (2) and step (4) of Example 1 were characterized.

[0097] (1) Get the DNP and MDNP prepared in Example 1 step (2) and step (4), configure it into an aqueous solution of 0.5mg / mL, carry out ultraviolet-visible absorption spectrum (UV-vis) test, measure the ultraviolet absorption, the result Such as image 3 shown. The UV results show that Au nanoparticles have an absorption peak around 510nm, which indicates the successful synthesis of gold nanoparticles;

[0098] (2) In order to characterize the morphology and size of the prepared nanoparticles, 1 mg of DNP and MDNP prepared in step (2) and step (4) of Example 1 of the present invention were dissolved in 1 mL of ultrapure water Prepare a suspension of nanoparticles in the medium, and take 5 μL of the suspension and drop it on the surface of the copper grid for TEM testing (such as Figure 4 shown). TEM results showed that DNP( Figure 4 A) and MDNP ( Figure 4 B) The size is unif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a dendritic cell-targeted hybrid dendrimer / YTHDF1 siRNA compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing G5.NH<2>-Man; preparing G5.NH<2>-Man-PS; preparing {(Au<0>)<25>-G5.NH<2>-Man-PS<20>}DENPs; and preparing the dendritic cell-targeted hybrid dendrimer / YTHDF1 siRNA compound. The method is simple in operation process and mild in reaction conditions; and the prepared compound can be efficiently swallowed by dendritic cells without influencing the activity of the dendritic cells, can transfect the dendritic cells and silence the expression of YTHDF1, and is used for enhancing tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of hybrid nanomaterials for tumor immunotherapy and their preparation and application, in particular to a dendritic cell-targeted hybrid dendrimer / YTHDF1 siRNA complex and its preparation method and application. Background technique [0002] Immunotherapy has become one of the most popular cancer treatment strategies in recent years due to its powerful effect on tumor eradication and prevention of recurrence. Immunotherapy can kill tumor cells by enhancing autoimmune sensitivity, and has lower toxicity and longer efficacy than traditional treatments. Tumor neoantigens play an important role in generating spontaneous antitumor immune responses and predicting clinical response to immunotherapy. Although there are a large number of neoantigens in the patient's body, it is basically difficult to completely cure the tumor due to the failure to obtain a sufficient and durable anti-tumor immune response. Studies have found...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/59A61K31/7088A61K47/02A61K47/54A61P35/00A61P37/04B82Y5/00B82Y30/00B82Y40/00
CPCA61K47/6925A61K47/549A61K47/545A61K47/595A61K47/02A61K31/7088A61P35/00A61P37/04B82Y5/00B82Y30/00B82Y40/00
Inventor 史向阳欧阳智俊高悦杨瑞沈明武
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com