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Achiral magnetic control micro-stent robot as well as preparation method and application thereof

A non-chiral and robotic technology, applied in surgical robots, surgical manipulators, surgery, etc., can solve the problems of slow repair process, low stem cell utilization, stem cell death in the scaffold center, etc., and achieve low production cost and high efficiency in vivo joints Cavity-targeted therapy, low-cost effects

Pending Publication Date: 2021-09-28
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the utilization rate of stem cells in this method is low, the demand for stem cells is large, and the repair process is relatively slow.
In recent years, some scientific research teams have studied the use of macroscopic scaffolds or microscopic scaffolds to culture stem cells in vitro, and used surgery or injection methods for stem cell therapy, but macroscopic scaffolds will use invasive surgery and cause the death of stem cells in the center of the scaffold.

Method used

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  • Achiral magnetic control micro-stent robot as well as preparation method and application thereof
  • Achiral magnetic control micro-stent robot as well as preparation method and application thereof
  • Achiral magnetic control micro-stent robot as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] In this embodiment, the achiral magnetically controlled micro-stent robot is prepared, and the preparation process is as follows figure 1 As shown, the following steps are specifically included:

[0062] (1) Photolithography manufacturing of the main body of the mesh stent

[0063] Spin-coat a 10% concentration of dextran solution on a clean silicon wafer at an angular speed of 1500 rpm for 20 s. After spin-coating, bake at 80°C for 1min, then bake at 180°C for 30min, and dry to form a film. Coat SU-8 2050 type photoresist, spin coating angular speed is 3000rpm, duration is 30s. The coated silicon wafers were baked at 65°C for 1 min. Fix the sample on a stage with a slope of 45°, place a mask on the rubber surface, expose it under vertical ultraviolet rays, then rotate the stage 90° clockwise (counterclockwise), and perform exposure with the same parameters again. Repeat the steps of rotation and exposure for a total of 4 exposures. The exposed samples were baked a...

Embodiment 2

[0067] In this embodiment, a micro-scaffold robot loaded with stem cells is prepared, and the preparation process is as follows: Figure 4 As shown, it specifically includes the following steps:

[0068] (1) PLL coating

[0069] Put the achiral magnetically controlled micro-stent robot into the PLL solution of 10 micrograms per milliliter and soak for 10 minutes. The samples were taken out, washed 6 times with 10% PBS solution, and dried naturally.

[0070] (2) Stem cell inoculation

[0071] After counting the number of suitable stem cells, they were inoculated on the achiral magnetically controlled micro-stent robot treated as above, cultured for 1 week, and a CCK-8 test was performed every 1, 3, and 7 days. Prepare the cell suspension, divide it into two groups, and inoculate one group into a 96-well plate after counting, 100 μl per well, at 37°C, 5% CO 2 environment for 24 h adherent culture. Add 10 μl of CCK-8 solution to each well, incubate for 2 hours, measure the a...

Embodiment 3

[0078] In this embodiment, the performance test is carried out on the micro-scaffold robot loaded with stem cells:

[0079] After the achiral magnetically controlled micro-scaffold robot is loaded with cells, it is driven by a magnetic field. It can move in DI water or different biological fluids with good movement speed, and has two different movement characteristics under the control of two kinds of magnetic fields. Achiral magnetically controlled micro-stent robots can carry out long-distance target movement, and can maintain good movement speed and stability on the bottom surface with three-dimensional structure and rough bottom surface, and easily climb over obstacles. The specific performance test method is as follows:

[0080] (1) Exercise test:

[0081] Place the achiral magnetically controlled micro-stent robot in DI water, apply a magnetic field, perform swimming and rolling tests, check whether it can move, and observe the speed and stability of movement.

[0082]...

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Abstract

The invention provides an achiral magnetic control micro-stent robot as well as a preparation method and application thereof. The achiral magnetic control micro-stent robot comprises a net-shaped stent main body, a magnetic film layer located on the outer layer of the net-shaped stent main body and a biological compatibility film layer located outside the magnetic film layer. The achiral magnetic control micro-stent robot stent structure can be used for attachment, growth, proliferation and differentiation of stem cells, has the effect of protecting and supporting the stem cells, can achieve directional movement under the control of a rotating magnetic field, and further can achieve targeted therapy on the affected part of osteoarthritis.

Description

technical field [0001] The invention belongs to the technical field of micro-nano robots, and relates to an achiral magnetically controlled micro-stent robot and its preparation method and application. Background technique [0002] Among various degenerative joint diseases, osteoarthritis (OA) is the most common chronic disease, which affects all joint tissues in the whole body. The main symptoms are knee joint pain and limitation of daily activities, and the prevalence rate of women is higher than that of men, and the prevalence rate of obese people is higher than that of the general population. [0003] KOA is a disease characterized by persistent articular cartilage damage and destruction, resulting in thickening of articular subchondral bone, varying degrees of joint synovial inflammation, and ligament degeneration. At present, the pathogenesis of KOA has not been clarified clinically, so there is no targeted prevention and treatment plan. The treatment options for KOA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M37/00A61B34/30
CPCA61B34/30A61B34/73A61B34/72A61M37/0069A61B2034/731
Inventor 郑裕基陈志丁惠钊
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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