Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bladder targeted drug-loaded exosome and application as well as drug for treating bladder diseases

A technology of bladder disease and exosomes, applied in the field of biomedicine, can solve problems such as increasing drug costs, lack of tissue targeting, and difficulty in achieving targeting effects, so as to improve therapeutic effects, reduce drug side effects, and facilitate application foreground effect

Inactive Publication Date: 2021-09-17
姜海涛
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanocarriers based on liposomes or albumin cannot exist stably in blood, are easily cleared by the reticuloendothelial system or mononuclear phagocyte system, and lack tissue targeting; antibody-modified carriers increase drug costs and are difficult to reach Ideal targeting effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bladder targeted drug-loaded exosome and application as well as drug for treating bladder diseases
  • Bladder targeted drug-loaded exosome and application as well as drug for treating bladder diseases
  • Bladder targeted drug-loaded exosome and application as well as drug for treating bladder diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Normal human embryonic kidney cells HEK-293 were collected (this example takes normal human embryonic kidney cells HEK-293 as an example). Take the culture medium of normal human embryonic kidney cells HEK-293 (kidney cell culture medium is cultured with exosome-free serum and DMEM medium) and centrifuge at 2000g centrifugal force for 10min, take the supernatant; then centrifuge at 10000g centrifugal force for 30min , take the supernatant, discard the cell debris and sediment; centrifuge the centrifuged cell culture solution at 100000g (g is the acceleration of gravity) centrifugal force for 2h, resuspend the precipitate with sterile PBS and store it for a short time at 4°C to obtain Bladder targeting exosomes. The extracted and isolated bladder-targeted exosome surface marker protein and particle size distribution are as follows: figure 1 , figure 2 shown. The expression of exosomes secreted by kidney cells was measured, and the concentration of exosomes protein se...

Embodiment 2

[0029] The exosomes secreted by normal human embryonic kidney cells HEK-293 were collected and stained with PKH67 to track the distribution of exosomes secreted by kidney cells in the body. The specific steps are as follows:

[0030] Take 100 μg of exosomes secreted by kidney cells HEK-293, incubate with 1 μL of PKH67 overnight at 4°C in the dark, centrifuge at 100,000 g (g is the acceleration of gravity) for 2 h, discard the supernatant, wash twice with PBS, no Exosomes secreted by kidney cells were resuspended in PBS and injected into C57bl / 6 mice through the tail vein. In this example, male C57bl / 6 (4-6 weeks) mice were purchased from Beijing Huafukang Biotechnology Co., Ltd. Co., Ltd., all mice were bred in SPF facilities; 24 hours later, the mice were anesthetized, and the liver, lung, spleen, bladder, kidney, stomach and intestines of the mice were taken to make frozen sections. After the nuclei were stained with Hoechst33342, the secretion of kidney cells was observed. ...

Embodiment 3

[0032] In this example, gemcitabine hydrochloride, a drug used to treat bladder diseases, was selected.

[0033] In this example, drugs for treating bladder diseases are introduced into bladder-targeted exosomes to prepare drugs for bladder tumor-targeted therapy. The specific operations are as follows:

[0034] 1) Take 150 μg of bladder-targeted exosomes and mix them with gemcitabine hydrochloride. The electroporation buffer can be (PBS, DMEM, Cytomix, Tris-HCl) to make up to 150 μL, and transfer to different specifications (0.2cm, 0.4cm) electroporation cups middle. Use different waveforms (exponential wave, square wave) and different voltages (50V, 100V, 200V, 300V) to perform electroporation on bladder-targeted exosomes and gemcitabine hydrochloride;

[0035]2) The electroporation product was ultracentrifuged for 120 min under a centrifugal force of 100,000 g, and the supernatant was collected to measure the drug loading. Results: For gemcitabine hydrochloride, 150 μg bl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biological medicine, and discloses a bladder targeted drug-loaded exosome and application as well as a drug for treating bladder diseases. The drug for treating bladder diseases is introduced into the exosome with bladder tissue targeting property to obtain the exosome, and the exosome with bladder tissue targeting property is derived from kidney cells; and a bladder tumor treatment drug contains the bladder targeted drug-loaded exosome. Compared with the prior art, the exosome secreted by the kidney cells can be enriched in the bladder tissue without any modification, the exosome derived from the kidney cells is used, the problem of exosome yield is solved, and the application prospect is very good; and the exosome from the kidney cells can be used for loading different drugs or active molecules, through targeted bladder tissue administration, the treatment effect on bladder diseases is improved, and toxic and side effects of the drugs are reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a bladder-targeted drug-loaded exosome and a drug for the application and treatment of bladder diseases. Background technique [0002] The bladder is the organ for storing and excreting urine in the human body. If the bladder loses the above-mentioned normal functions for any reason, there will be abnormalities in the frequency of urination, urine volume and urine color, and bladder diseases will occur. Common bladder disorders include bladder inflammation, bladder polyps, bladder muscle weakness, and bladder cancer. Bladder cancer is the most common malignant tumor of the urinary system and one of the ten most common tumors in the whole body. It accounts for the first place in the incidence of genitourinary system tumors in my country, and its incidence rate is second only to prostate cancer in the West, ranking second. [0003] At present, whether it is chemical drugs or ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/46A61K45/00A61P13/10
CPCA61K9/5068A61K45/00A61P13/10
Inventor 姜海涛王天怡
Owner 姜海涛
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com