Application of bifidobacterium lactis JYBR-190 in product for removing heavy metals in body
A technology of JYBR-190 and Bifidobacterium lactis, applied in the fields of application, function of food ingredients, bacteria, etc., can solve problems such as loss of beneficial metal ions, inability to actively remove arsenic treatment, and many adverse reactions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] A kind of bifidobacterium lactis JYBR-190, the screening process of this bacterial strain JYBR-190 is as follows:
[0029] (1) Preparation of MRS plate medium: peptone 10g, beef powder 5g, longibacterium powder 4g, K 2 HPO 4 ·7H 2 O 2g, triammonium citrate 2g, sodium acetate trihydrate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O0.05g, 15g agar, 1000mL distilled water, mix well, sterilize at 121°C for 20min, let cool, and set aside;
[0030] (2) Isolation and identification of strains:
[0031] 1) Weigh 5g of naturally fermented yogurt from a herdsman's home in Yili Prefecture, Xinjiang under aseptic conditions, add the yogurt to 45mL of 0.85% sterilized physiological saline containing glass beads, and mix well to obtain a mother liquor;
[0032] 2) Shake the mother solution for 10 minutes at a speed of 1500 rpm, and then take 1mL of the mother solution under sterile conditions to dilute with a 10-fold gradient to form a dilution; choose 10 ...
Embodiment 2
[0036] The preparation process of Bifidobacterium lactis JYBR-190 bacterium powder is as follows:
[0037] (1) Pick a single colony of newly cultured Bifidobacterium lactis JYBR-190 from the MRS plate in Example 1, inoculate it in MRS liquid medium with an inoculum size of 1%, and culture it at 37°C for 24 hours to obtain bacteria liquid;
[0038] Among them, the MRS liquid medium includes peptone 10g, beef powder 5g, longibacterium powder 4g, K 2 HPO 4 ·7H 2 O2g, triammonium citrate 2g, sodium acetate trihydrate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O 0.05g, distilled water 1000mL;
[0039] (2) Using aseptic operation, the bacterial solution was centrifuged and washed with sterile saline, then resuspended in 10% (w / w) sterile reconstituted skim milk, and the concentration was adjusted to 1.0×10 10 cfu / mL, obtain the bacterial suspension, freeze-dry the bacterial suspension to obtain the freeze-dried powder;
[0040] (3) Mix the freeze-dried ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com