Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof

A technology of microbial strains and insecticides, applied in the direction of application, fungi, biocides, etc., can solve the problems of slow insecticidal effect, large environmental impact, and unstable effect

Pending Publication Date: 2021-07-23
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, Beauveria bassiana has the problems of slow insecticidal effect, large environmental impact and unstable effect in practical application.

Method used

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  • Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof
  • Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof
  • Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the collection of soil sample and the separation and purification of fungus

[0021] Collect soil samples, take about 100g of soil at 10-15cm below the topsoil, pack it in a plastic bag and bring it back to the laboratory for processing. Sieve the soil sample to remove stones and sundries, then take 10g of pure soil and suspend in 90mL 0.1% Tween 80 solution, shake well, let it stand for 15min, take 2mL of its supernatant and dilute it in 8mL 0.05% Tween 80, Prepared as a soil suspension. Inoculate 0.1mL soil suspension on Bengal red agar medium plate, push the suspension evenly under the surface of the plate with a triangular glass scraper, incubate at 25°C for 3-7 days, then cut out a single colony with an inoculation loop, and inoculate it on a PDA plate Continue to cultivate. A total of 114 strains of various genera were collected and isolated, and the mycelium pieces of each strain were cut and transplanted on a PDA slope, continued to be cultivated...

Embodiment 2

[0025] Embodiment two, the screening of highly virulent strain

[0026] 1. Preparation of spore suspension

[0027] Take the collected entomogenous fungi that have been cultured for about 10-14 days, add distilled water containing 0.5% Tween-80 in the ultra-clean workbench to scrape off the colonies, wash out the spores in the plate, and transfer them to a sterilized Erlenmeyer flask. Use a constant temperature magnetic stirrer to stir at a medium speed for 20 minutes to make it evenly dispersed and prepare a mother liquor. According to the 10-fold dilution method, the bacterial solution was diluted 100 times, and the number of conidia was counted with a hemocytometer under a microscope, and repeated on 5 plates. The conidia concentration was then diluted to 1 × 10 8 Spores / mL for later use.

[0028] 2. Toxicity determination of isolated and purified bacterial strains against Spodoptera litura

[0029] The virulence of the isolated and purified 114 strains was tested by di...

Embodiment 3

[0040] Example 3. Further analysis of the virulence of bacterial strain SCAUYZ-16

[0041] 1. Concentration gradient analysis of highly virulent strains

[0042] The strain SCAUYZ-16 with good virulence effect was selected, and its spore powder was taken to prepare a concentration of 1.00×10 8 spore / mL spore suspension and diluted according to a certain gradient, the final dilution concentration was 1.00×10 8 spores / mL, 1.00×10 7 spores / mL, 1.00×10 6 spores / mL, 1.00×10 5 spores / mL, 1.00×10 4 spores / mL spore suspension, and the mortality rate of Beauveria bassiana strain SCAUYZ-16 to the 2nd instar larvae of Spodoptera litura under different concentrations was determined by dipping method. According to the relationship between concentration and mortality, a regression equation was established to determine LC50. The formula for calculating the mortality rate and corrected mortality rate of the 2nd instar larvae of Spodoptera litura is as follows:

[0043] Mortality rate (...

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Abstract

The invention belongs to the field of plant protection and particularly relates to the field of biological control. An excellent strain SCAUYZ-16 with high pathogenicity to prodenia litura is obtained through screening research, the SCAUYZ-16 is preserved in Guangdong Microbial Culture Collection Center on November 19, 2020, and the preservation number is GDMCC No: 61300. Experiments show that the virulence of the strain at 10<8> spores / mL to second-stage larvae of prodenia litura reaches 70.71%, the pathogenicity of the strain is more stable than that of other tested strains, and the strain has great potential to be used for biological control of prodenia litura.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to the field of biological control, in particular to the biological control of Spodoptera litura. Background technique [0002] According to public information (FAO, 2015), from 2000 to 2015, the total use of pesticides in China increased by 39.35%. At present, China has become one of the largest pesticide producers and consumers in the world. 1.5 and 4.0 times the world average, respectively, the widespread use of pesticides in modern agriculture has led to agricultural non-point source pollution of rivers and streams worldwide, threatening drinking water resources and aquatic ecosystems (Jargentz, S., Mugni, H. ,Bonetto,C.,Schulz,R.,2005.Assessment of insecticide contamination in runoff and stream water of small agricultural streams in the main soybean area of ​​Argentina.Chemosphere61,817-826.) Pesticides on fish, algae and aquatic Invertebrate toxicity has also been reported (L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 吴建辉乐扬陈月银李佳颖袁芳周向平肖卡特·阿里邱宝利
Owner SOUTH CHINA AGRI UNIV
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