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Application of selenium preparation in preparation of medicine for treating Crohn disease

A Crohn's disease and preparation technology, which is applied to the application field of selenium preparations in the preparation of medicines for treating Crohn's disease, can solve the problems such as the treatment effect of selenium supplement preparations that have not been disclosed in the literature, and achieve the improvement of enteritis symptoms and the promotion of remission. Effect

Pending Publication Date: 2021-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature disclosing the therapeutic effect of selenium supplementation preparations in inflammatory bowel disease

Method used

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  • Application of selenium preparation in preparation of medicine for treating Crohn disease
  • Application of selenium preparation in preparation of medicine for treating Crohn disease
  • Application of selenium preparation in preparation of medicine for treating Crohn disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1 in vitro cell experimental implementation

[0024] 1.1 Mouse initial CD4 + T cell acquisition:

[0025] The mice were separated from the cervical spot, separated from the underarm, the mandibular lymph nodes and spleen, treated with a syringe to ground lymph nodes and spleen, filtrate, centrifugal, 180 ul rpmi + 20 μL anti-mouse CD4 magnetic bead, 4 ° C incubation 15 Minutes; 2 ml of washing; CD4 + T cells were selected by the LS column. The collected cell fluid was resuspended with 100 μl of 10% FBS RPMI, and antibody FITC ANTI-MOUSE CD44 was added, 1 μl of APC Anti-Mouse Cd62L was added to 4 ° C. Add 10% FBS RPMi2ML to centrifuge cells, discard it, resuspended to 600 μL, sorted by Arial II stream sorter, obtained CD44 lo CD62L hi The population is initial CD4 + T cell.

[0026] 1.2 T cell differentiation experiment:

[0027] The Anti-CD3 / Anti-CD28 package was 24-well plate, 4 ° C overnight. The initial T cells obtained according to the above steps are 4 × 10 5 The wel...

Embodiment 2

[0031] 2 animal enteritis model implementation

[0032] 2.1 experiment group

[0033] Piece method 1: 1 control group (normal diet drinking water); 2 selenium group (0.8 ppm of sodium selenite in drinking water).

[0034] Group 2: RAG1 - / - Mouse fed selenium feed (0 ppm) 4-8 weeks, establish a selenium in vivo, and then divided into two groups, 1 group continued to maintain selenium feed to the end of manufacturing, 2 other colitis model After the addition of selenium diet (0.2 ppm) and sodium selenate (0.8 ppm).

[0035] 2.2 Experimental Process

[0036] CD4 of a wild-type mouse by using a magnetic bead sorting a streaming cytometry + CD45RB hi T cells, end-tail intravenous injection RAG1 - / - Mouse (5 × 10 5 / only). Weigh the weight per week, and complete the model after 6-8 weeks. Mouse weight, statistical mouse mortality, etc. during modeling. After the mold, mice were sacrificed, and the length of the intestine was measured, and the tissue specimens at the anus 1, 3, 8 cm, and...

Embodiment approach

[0041] 1 Study population:

[0042] standard constrain

[0043] 1 age 18-60 years old;

[0044] 2 Combined with medical history, physical examination, auxiliary examination, etc. confirmed the diagnosis of Coron disease patients.

[0045] 3 Agree to detect the peripheral blood selenium level, and the selenium concentration is less than 84μg / L

[0046] 4 Agree to participate in this study and sign this research informed consent

[0047] Exclude standard:

[0048] 1 The combined person suffers from any unstable or uncontrolled cardiovascular, lung, liver, kidney, gastrointestinal tract, urinary hematology, coagulation function, immunization, endocrine / metabolism or other medical diseases, and researchers believe that the results of the interference Or endanger the safety of the subject.

[0049] 2 The results of lactating female subjects or serum pregnancy test results are positive.

[0050] 3.2 Administration / Treatment / Observation Program:

[0051] Serum selenium concentrat...

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Abstract

The invention provides application of a selenium preparation in preparation of a medicine for treating Crohn disease. In-vitro cell experiment research proves that the sodium selenite has a good effect of inhibiting Th1 differentiation when the concentration of the sodium selenite is controlled to be 0.5-1 mu M, and has no obvious toxicity to cells. The sodium selenite has a remarkable improvement effect on enteritis symptoms induced by adoptive transfer of mouse T cells, Th1 cells in mouse colon tissue can be remarkably inhibited, and small-range clinical tests show that the sodium selenite can promote relieving of enteritis of patients with selenium-deficient Crohn disease. The invention provides new application of sodium selenite, which is of great significance to research and development of medicines for treating Crohn disease and can provide reference for development and mechanism research of clinical medicines.

Description

Technical field [0001] The present invention relates to the application of selenium preparations in the preparation of the treatment of Croan disease drugs. Background technique [0002] Inflammatory Bowel Disease (IBD) mainly includes Crohn'sDisease, CDs and ulcerative colitis (UC), is a group of chronic non-specific intestinal inflammatory diseases. In recent years, IBD has grown rapidly in China, which repeatedly, seriously affecting the quality of life of patients, causing a heavy social medical economic burden. CD and UC have significant differences in epidemiology, genetic characteristics, clinical manifestations and histopathological changes. [0003] The main manifestation of Crohn disease is abdominal pain, diarrhea, weight loss, fever, etc., endoscopic, longitudinal ulcer, pebble-like change, pathological manifestation is transmissive inflammation, non-cheese granulation, Ulcerative colitis is expressed as mucus pus, abdominal pain, etc., endoscopic, pouring irrigation ...

Claims

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Application Information

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IPC IPC(8): A61K33/04A61K31/198A61K36/064A61P1/00A61P29/00
CPCA61K33/04A61K31/198A61K36/064A61P1/00A61P29/00
Inventor 靳津曹倩黄灵洁毛信韬李异媛
Owner ZHEJIANG UNIV
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