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Method for evaluating virus inactivation efficiency of virus inactivation mask

A virus inactivation and face mask technology, which is applied in biochemical equipment and methods, and microbial measurement/inspection, can solve the problems that the inactivation effect of virus inactivation masks cannot be evaluated, so as to promote development and innovation, reduce the risk of infection, and ensure authenticity effect

Pending Publication Date: 2021-06-29
陕西省医疗器械质量检验院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for evaluating virus inactivation mask to virus inactivation efficiency, overcome the deficiency that the inactivation effect of virus inactivation mask cannot be evaluated, realize the mensuration of virus inactivation efficiency of virus inactivation mask, effectively promote The quality supervision and development innovation of virus inactivated masks will enhance the protection of medical staff and the safety protection of ordinary residents, and reduce the risk of infection

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  • Method for evaluating virus inactivation efficiency of virus inactivation mask
  • Method for evaluating virus inactivation efficiency of virus inactivation mask
  • Method for evaluating virus inactivation efficiency of virus inactivation mask

Examples

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Effect test

Embodiment 1

[0056] Taking the indicator virus as Sindbis virus (Sindbis virus) as an example, a method for evaluating the virus (Sindbis virus) inactivation efficiency of a virus inactivation mask, the corresponding host cell is a Vero cell, including the following steps:

[0057] S1. After the Vero cells in the logarithmic growth phase were digested with trypsin and counted, MEM medium containing 10% fetal bovine serum was added to adjust the cell density to 5×10 5 cells / mL to obtain cell suspension, take 15mL of cell suspension into T75 cell culture flask and place at 37°C, 5% CO 2 Culture in an incubator. When the cells reach 90% confluence, discard the medium in the T75 cell culture flask, add 13 mL of MEM medium containing 10% fetal bovine serum, and continue to culture the cells to a super confluent state;

[0058] Discard the medium in the T75 cell culture flask, first add 1 mL of Sindbis virus liquid to the T75 cell culture flask, then add 3 mL of MEM medium containing 10% fetal b...

Embodiment 2

[0077] A method for evaluating virus inactivation mask to virus (Sindbis virus) inactivation efficiency, corresponding host cell is Vero cell, comprises the following steps:

[0078] S1. After the Vero cells in the logarithmic growth phase were digested with trypsin and counted, MEM medium containing 10% fetal bovine serum was added to adjust the cell density to 5×10 5 cells / mL to obtain cell suspension, take 15mL of cell suspension into T75 cell culture flask and place at 37°C, 5% CO 2 Culture in an incubator. When the cells reach 90% confluence, discard the medium in the T75 cell culture flask, add 14 mL of MEM medium containing 10% fetal bovine serum, and continue to culture the cells to a super confluent state;

[0079] Discard the culture medium in the T75 cell culture flask, first add 1 mL of Sindbis virus solution to the T75 cell culture flask, then add 3.5 mL of MEM medium containing 10% fetal bovine serum, shake the T75 cell culture flask to remove Sindbis virus Afte...

Embodiment 3

[0098] A method for evaluating virus inactivation mask to virus (Sindbis virus) inactivation efficiency, corresponding host cell is Vero cell, comprises the following steps:

[0099] S1. After the Vero cells in the logarithmic growth phase were digested with trypsin and counted, MEM medium containing 10% fetal bovine serum was added to adjust the cell density to 5×10 5 cells / mL to obtain cell suspension, take 15mL of cell suspension into T75 cell culture flask and place at 37°C, 5% CO 2 Culture in an incubator. When the cells reach 90% confluence, discard the medium in the T75 cell culture flask, add 15 mL of MEM medium containing 10% fetal bovine serum, and continue to culture the cells to a super confluent state;

[0100] Discard the culture medium in the T75 cell culture flask, first add 1 mL of Sindbis virus liquid to the T75 cell culture flask, then add 4 mL of MEM medium containing 10% fetal bovine serum, shake the T75 cell culture flask to make the Sindbis virus liquid ...

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Abstract

The invention relates to the technical field of virus protection, in particular to a method for evaluating the virus inactivation efficiency of a virus inactivation mask. The method comprises the following steps: infecting host cells with viruses, culturing to obtain virus-containing cells, freezing and thawing the virus-containing cells, centrifuging, taking supernate, filtering to obtain a virus suspension, preparing the virus suspension into aerosol particles, simulating the respiratory action of a human body, enabling the aerosol particles to be in full contact with a virus inactivation mask, then preparing a virus test solution, infecting host cells by using the virus test solution diluted in a gradient manner, observing and recording the well number of diseased cells, taking a common medical mask without sterilization and antiviral components as a positive control group, treating as above, and calculating the inactivation efficiency of the virus inactivation mask according to the virus titer values of the virus test solution of the sample group and the positive control group. On the basis of safety, the authenticity of simulation is guaranteed, the defect that the inactivation effect of the mask cannot be evaluated is overcome, development and innovation of the virus inactivation mask are effectively promoted, the protection effect is enhanced, and the infection risk is reduced.

Description

technical field [0001] The invention relates to the technical field of virus protection, in particular to a method for evaluating the virus inactivation efficiency of a virus inactivation mask. Background technique [0002] At present, the respiratory system diseases caused by viruses are widely concerned all over the world, and the transmission routes, treatment methods and protective means of such diseases have also become the hotspots of competing researches in various countries. The key to infectious diseases is that people usually wear medical masks to isolate their mouth and nose from the external risk environment. However, although ordinary medical masks rely on multi-layer filter materials with extremely small pores to absorb and filter bacteria and viruses, they can greatly reduce the risk of infection , but it does not guarantee 100% filtration of bacteria or viruses, especially the long-term exposure and accumulation of medical staff in high-risk environments, whi...

Claims

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Application Information

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IPC IPC(8): C12Q1/22
CPCC12Q1/22
Inventor 王妙张成韩溟刘东鸣刘博何晴丁鸽陈博宇王亚琪王晓东许文锋王燕慧明文娟
Owner 陕西省医疗器械质量检验院
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