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Essence containing mesenchymal stem cell factors and preparation method thereof

A technology of stem cell factor and mesenchymal stem cell, applied in the field of essence containing mesenchymal stem cell factor and its preparation, can solve the problems of low anti-wrinkle ability and poor repair effect

Pending Publication Date: 2021-06-29
春日花开生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide an essence containing mesenchymal stem cell factor and its preparation method, aiming to solve the problems of poor repair effect and low anti-wrinkle ability caused by adding stem cell factor alone in the prior art

Method used

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  • Essence containing mesenchymal stem cell factors and preparation method thereof
  • Essence containing mesenchymal stem cell factors and preparation method thereof
  • Essence containing mesenchymal stem cell factors and preparation method thereof

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preparation example Construction

[0040] Correspondingly, the embodiment of the present invention also provides a method for preparing an essence containing mesenchymal stem cell factors. The method comprises the steps of:

[0041] S01. Weigh each component according to the essence containing mesenchymal stem cell factor;

[0042] S02. Add the glycerin, the trehalose, the sodium hyaluronate, the carbomer, and the preservative into the pure water, heat to 60-65°C and mix to obtain a first composition;

[0043] S03. Cool the first composition to 20-25°C, add the butanediol, the biopolysaccharide gum, the hydrolyzed collagen, the vitamin A acetate, and the vitamin E acetate, and mix to obtain second composition;

[0044] S04. Lower the temperature of the second composition to 2-8° C., add the stem cell factor solution and the stem cell exocrine, and mix to obtain the essence containing mesenchymal stem cell factor.

[0045] Specifically, the preferred contents and types of the components of the essence contain...

Embodiment 1

[0083] Preparation of umbilical cord mesenchymal stem cells

[0084] The preparation method of the umbilical cord mesenchymal stem cells comprises the following steps:

[0085] (1) Provide primary cells of umbilical cord mesenchymal stem cells; the specific operation is as follows: operate in an ultra-clean workbench, take sterile fresh umbilical cord, and use normal saline containing gentamicin Wash the fresh umbilical cord mentioned above to remove the blood attached to its surface. At the same time, the blood vessels on the umbilical cord are stripped, and the umbilical cord stroma tissue with the blood vessels stripped is washed with normal saline containing gentamicin until it flows through the sieve. After the liquid is not viscous, soak in the culture medium for cultivation. Cut the umbilical cord matrix tissue into pieces of about 3-5mm 3 Add 5-8 mL of serum-free culture medium to tissue pieces of different sizes, and culture them at 37°C in an incubator with a volum...

Embodiment 2

[0090] Preparation of Stem Cell Factor Solution

[0091] The preparation method of stem cell factor solution comprises the following steps:

[0092] (1) Provide umbilical cord mesenchymal stem cells for cultivation to obtain a first culture system, centrifuge the first culture system to collect the cell bodies of the first culture system, wash the cell bodies of the first culture system with PBS solution, and then The cell pellet was collected by centrifugation at 1000 rpm for 5 min. Repeat the above washing steps 2 to 3 times to obtain cell bodies, and select physiological saline to dissolve the cell bodies to prepare a cell body suspension; wherein, the density of the cell body suspension is 5×10 7 / mL.

[0093] (2) The cell body suspension is prepared by freezing and thawing with liquid nitrogen to obtain a stem cell factor solution, and the method for freezing and thawing with liquid nitrogen includes the following steps: placing the cell body suspension in a liquid nitr...

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Abstract

The invention provides essence containing mesenchymal stem cell factors. The essence containing the mesenchymal stem cell factors is prepared from the following components in parts by weight: 3 to 8 percent of glycerol, 3 to 10 percent of butanediol, 2 to 7 percent of biological polysaccharide gum, 1 to 4 percent of trehalose, 0.5 to 1 percent of sodium hyaluronate, 0.1 to 1 percent of hydrolyzed collagen, 0.1 to 1 percent of vitamin A acetate, 0.1 to 1 percent of vitamin E acetate, 0.05 to 0.3 percent of carbomer, 0.5 to 1 percent of a preservative, 0.5 to 2 percent of a stem cell factor solution, 0.5 to 2 percent of stem cell exosome and the balance pure water, wherein the weight of the essence containing the mesenchymal stem cell factors is 100 percent. The essence containing the mesenchymal stem cell factors can be used for enhancing the repairing of skin and inhibiting a reaction of excessive inflammation of the skin, so that the repairing effect and the anti-wrinkle capability are improved.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to an essence containing mesenchymal stem cell factor and a preparation method thereof. Background technique [0002] As time goes by, the skin will age, and the aging of the skin is affected by endogenous and exogenous factors. Endogenous factors include physiological, endocrine and genetic factors, also known as time-course aging. Exogenous factors include environmental influences such as air pollution, sunlight and ultraviolet rays, smoking, chemical exposure, and malnutrition. Exogenous aging is also known as photoaging. Photoaging results in thinning of the skin, degradation of collagen, deposition of denatured elastic fibers, and depigmentation of wrinkled and yellow skin. In addition, photoaging generates free radicals that activate matrix metalloproteinases, which in turn degrade the extracellular matrix. When the dermis of the skin becomes thinner, the elasticity of the skin bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/98A61K8/64A61K8/73A61K8/34A61K8/65A61K8/67A61K8/81A61Q19/08
CPCA61K8/99A61K8/982A61K8/64A61K8/735A61K8/345A61K8/65A61K8/671A61K8/678A61K8/8147A61Q19/08A61K2800/805A61K2800/592
Inventor 黎绵生谢崇辉
Owner 春日花开生物科技(深圳)有限公司
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