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Metarhizium rileyi and biological control method and application of Metarhizium rileyi in pupal stage of spodoptera frugiperda

A technology of Spodoptera frugiperda and Metarhizium anisopliae, applied in the field of microorganisms

Active Publication Date: 2021-06-25
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no underground control fungal agent for this insect. If a fungal agent can be developed to prevent and control in the pupal stage, it will be able to effectively control the population of Spodoptera frugiperda, which is of great significance to the control of Spodoptera frugiperda

Method used

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  • Metarhizium rileyi and biological control method and application of Metarhizium rileyi in pupal stage of spodoptera frugiperda
  • Metarhizium rileyi and biological control method and application of Metarhizium rileyi in pupal stage of spodoptera frugiperda
  • Metarhizium rileyi and biological control method and application of Metarhizium rileyi in pupal stage of spodoptera frugiperda

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Isolation and identification of Metarhizium anisopliae FJMR2 bacterial strain

[0039] Isolation, purification and pathogenicity determination of 1FJMR2 strain

[0040] Metarhizium anisopliae FJMR2 of the present invention was collected on August 6, 2019 from the larvae of the diseased Spodoptera moth in the corn planting area of ​​Dongfeng Town, Jian'ou City, Fujian Province. Put the collected carcasses in a 5mL sterile EP tube and bring them back, and gently shake the carcasses on PDA medium (200g of potatoes, 20g of glucose, 20g of agar, 1000mL of distilled water) until some conidia fall on the medium , cultured in a biochemical incubator at 26°C, and after sporulation, the conidia were transferred to a new PDA medium for purification to obtain the FJMR2 strain.

[0041] The purified FJMR2 strain was cultivated in a biochemical incubator at 26°C with SMAY medium (40 g maltose, 10 g peptone, 2 g yeast extract, 20 g agar, and 1000 mL distilled water). T...

Embodiment 2

[0046] Example 2: Infection characteristics of Metarhizium anisopliae FJMR2 to larvae of Spodoptera frugiperda at different ages

[0047] a. Inoculate Metarhizium anisopliae FJMR2 on SMAY solid slant medium, culture at 26°C for 7-9 days, and collect spores after the culture;

[0048] b. Dip a small amount of spore powder with a hairbrush, and brush it on the body wall of young larvae (1-3 instar larvae) and advanced age larvae (4-5 instar) Spodoptera frugiperda larvae respectively, cultivate them at 26°C for 7-12 days, and observe Metarhizium anisopliae FJMR2 Infection characteristics of Metarhizium anisopliae FJMR2 on different ages of Spodoptera frugiperda larvae.

[0049] Results: The young larvae could appear as early as the 4th day and died due to the infection of Metarhizium anisopliae. In the early stage of death, the surface of the larvae was covered with a thin layer of hyphae. In the later stage, the larvae shriveled and stiffened, and no spores appeared; The death ...

Embodiment 3

[0051] Embodiment 3: The pathogenicity determination of FJMR2 bacterial strain to Spodoptera frugiperda pupae

[0052] 1 Determination of the pathogenicity of different FJMR2 spore concentrations on Spodoptera frugiperda pupae

[0053] The culture method of Example 2 was used to collect Metarhizium anisopliae FJMR2 spores. The spores of Metarhizium anisopliae FJMR2 were prepared with an aqueous solution with a sterile mass percentage of 0.05% Tween-80 to a concentration of 3.00×10 8 Spores / mL, 3.00×10 7 Spores / mL, 3.00×10 6 Spores / mL and 3.00×10 5 Spore / mL spore suspension, 0.05% Tween-80 sterile water was used as blank control. Healthy 1-day-old Spodoptera frugiperda pupae of uniform size were selected for inoculation by dipping method. Place the pupae for testing in the prepared spore suspensions of different concentrations and immerse them for 5 seconds, take them out and dry them naturally, then place them in an incubator at 26°C, with a relative humidity of 70%, and ...

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Abstract

The invention provides Metarhizium rileyi and a biological control method and application of Metarhizium rileyi in a pupal stage of spodoptera frugiperda, and belongs to the technical field of microorganisms. The preservation number of the Metarhizium rileyi FJMR2 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.18553. The Metarhizium rileyiFJMR2 provided by the invention has strong pathogenicity to spodoptera frugiperda pupae, can obviously reduce the eclosion rate of the spodoptera frugiperda pupae, has higher teratogenesis rate and fatality rate to eclosion adults, can effectively control the increase of the population number of the spodoptera frugiperda, and is environment-friendly and safe to human and livestock. The Metarhizium rileyi FJMR2 provided by the invention can solve the technical problems that in the prior art, chemical pesticides are adopted for preventing and controlling the spodoptera frugiperda, the environment is polluted, drug resistance is easily generated, and underground pupae are difficult to prevent and control.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a metarhizium anisopliae and its biological control method and application in the pupal stage of Spodoptera frugiperda. Background technique [0002] Spodoptera frugiperda (Spodoptera frugiperda), commonly known as fall armyworm, belongs to the genus Spodoptera of the family Noctuidae. It is a major migratory and omnivorous agricultural pest that originated in the Americas. It mainly harms corn, rice, sugarcane It has the characteristics of wide suitable area, strong reproductive ability, and difficult control. At present, the prevention and control of Spodoptera frugiperda mainly relies on chemical pesticides, but this insect has strong resistance to pesticides, and excessive reliance and abuse of pesticides can easily cause damage to the environment and non-target organisms, and also make pests resistant to pesticides, increasing the difficulty of control . ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 丁雪玲何玉仙姚凤銮郑宇卢学松
Owner INST OF PLANT PROTECTION FAAS
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