Application of demethylzeylasteral in medicine for treating leucoderma and ointment prepared from demethylzeylasteral

A technology of norzeratin and vitiligo, applied in the field of biomedicine, can solve the problems of excessive toxic and side effects, complex chemical components, and difficulty in quality assurance, and achieve the effects of stable drug efficacy, high purity, and immune damage protection

Active Publication Date: 2021-06-11
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical composition of Tripterygium wilfordii is complex, so it is difficult to guarantee its quality when the medicinal material is extracted with a single ingredient as the index, especially some monomers such as triptolide and triptolide have the defects of excessive toxic side effects and strong mutagenicity , thus limiting the clinical application of Tripterygium wilfordii

Method used

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  • Application of demethylzeylasteral in medicine for treating leucoderma and ointment prepared from demethylzeylasteral
  • Application of demethylzeylasteral in medicine for treating leucoderma and ointment prepared from demethylzeylasteral
  • Application of demethylzeylasteral in medicine for treating leucoderma and ointment prepared from demethylzeylasteral

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Extraction and preparation of norzera woodaldehyde

[0034] S1: Take fresh Tripterygium wilfordii to clean the surface sludge, cut it into small pieces, and then clean it with distilled water. After drying under reduced pressure, the medicinal material of Tripterygium wilfordii is crushed, sieved, and cellulase and pectinase are added for enzymolysis. The amount of enzyme is 7% of the raw material mass, the enzymolysis temperature is 30°C, and the enzymolysis time is 1.5h. After the reaction solution was filtered, the filter residue was collected, 5 times the amount of 60wt% ethanol was added for ultrasonic extraction for 3 times, the extracts were combined, and concentrated to obtain an extract;

[0035] S2: Add an appropriate amount of lye with pH=8 to the extract in step S1 to dissolve, filter, take the filtrate and add acid to adjust to pH=7, then add 2 times the amount of ethyl acetate to extract 3 times, combine the extracts, evaporate Dried to get the...

Embodiment 2

[0037] Example 2: Norsera wood aldehyde affects CD8 in patients with vitiligo + T cell proliferation, activation and killing effect experiment

[0038] The peripheral blood of patients with vitiligo used in the experiment came from the Vitiligo Specialist Clinic of the Dermatology Department of Xijing Hospital. A total of 10 patients with non-segmental vitiligo were included in the peripheral blood. The inclusion criteria were:

[0039] 1) Conform to the criteria for the diagnosis, clinical classification and curative effect determination of vitiligo revised in 2014 by the Pigment Disease Group of Dermatology and Venereology of Traditional Chinese and Western Medicine;

[0040] 2) The subjects voluntarily participated in this study and signed the informed consent.

[0041] Exclusion criteria:

[0042] 1) Those suffering from organic dysfunction;

[0043] 2) Abnormal liver and kidney function;

[0044] 3) Suffering from other autoimmune diseases;

[0045] 4) Suffering from i...

Embodiment 3

[0058] Example 3: Cell experiment of treating primary human keratinocytes with norzeratinaldehyde

[0059] Weigh the norzeratin aldehyde extracted in Example 1, dissolve it in DMSO and then dilute it with 1640 medium to a concentration of 1 μM used in the experiment to obtain the 1640 medium containing norzeratin, which is used for norzeratin Cellular experiments of xylaldehyde-treated primary human keratinocytes.

[0060] After the keratinocyte SFM medium was resuspended, the cell pellet was transferred to a cell culture flask and kept at 37°C, 5% CO 2 Routine culture in a cell culture incubator. When the density of adherent cells increases to 80-90%, add 0.25% trypsin-EDTA digestion solution for differential digestion, observe the cell morphology continuously under an inverted microscope, remove melanocytes and fibroblasts, and conduct cell passage to identify the primary Human keratinocytes.

[0061] H&E (hematoxylin and eosin) staining was used to identify the purity of...

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Abstract

The invention belongs to the technical field of biomedicine, relates to an application of demethylzeylasteral in a medicine for treating leucoderma and an ointment prepared from demethylzeylasteral, and determines an effective treatment effect of a tripterygium wilfordii monomer demethylzeylasteral in the aspect of treating leucoderma. The active component demethylzeylasteral in the medicine is used for treating leucoderma by reducing generation and secretion of keratinocyte chemotactic factors CXCL9 / 10 / 16 under inflammatory stimulation and oxidative stress and inhibiting skin migration, activation, proliferation and differentiation of CD8 + T cells. The medicine can be prepared into an oral administration dosage form, a non-oral administration injection or an external dosage form, the prepared medicine is used for treating leucoderma, the ointment is low in toxicity and good in effect, and a foundation is laid for research and development of leucoderma targeted therapy medicines in the future.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of norzeratin in vitiligo medicine and its ointment. Background technique [0002] Vitiligo is a common chronic, acquired, autoimmune skin disease characterized by depigmentation of epidermal melanocytes. The typical clinical manifestations of its skin lesions are depigmented spots with clear borders on the skin and mucous membranes. The skin lesions can be distributed all over the body, usually around the mouth, around the eyes, fingers, dorsum of feet, etc., and the hair may turn white. . At present, the global incidence rate of this disease is about 0.5-1%, and it is reported that India has the highest incidence rate, about 8.8%, followed by Mexico, about 2.6-4%, and Japan incidence rate, about 0.06%-2.3%. A large-scale epidemiological survey in my country showed that the prevalence of vitiligo in Shaanxi in 2004 was about 0.09%. [0003] Vitiligo is a d...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61K36/37A61K9/06A61K47/36A61P17/00A61P37/02A61P19/02A61P17/06C07J63/00
CPCA61K31/56A61K36/37A61K9/06A61K47/36A61P17/00A61P37/02A61P19/02A61P17/06C07J63/008
Inventor 李春英常毓倩李舒丽杜鹏冉郭森高天文
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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