Preparation method of gene editing novel coronavirus vaccine vector

Abstract

The invention discloses a preparation method of a gene editing novel coronavirus vaccine vector, which is characterized in that tissue cells such as amniocytes, neonatal cord blood, umbilical cords and placentas, which are originally abandoned and left after prenatal diagnosis, are transformed into a recyclable novel coronavirus vaccine vector which have a stem cell natural treatment function and an infinite passage immortalization function and a novel coronavirus ACE2 receptor missing characteristic by methods of novel coronavirus susceptible receptor ACE2 gene editing, SV40LT and / or hTERT gene transfection; the gene editing novel coronavirus vaccine vector is specially used for preparing a vaccine for individualized treatment of COVID-19, and can be pre-stored in a stem cell library with a temperature of minus 196 DEG C for a long time according to names, ABO blood types or HLA typing; and when the novel coronavirus infectious disease breaks out or a certain individual needs to use the gene editing novel coronavirus vaccine vector, stem cell treatment or vaccine preparation can be carried out after passage and amplification of an autologous or homotype gene editing immortal stem cell line, so as to eliminate and reduce immunological rejection in traditional stem cell treatment and vaccine use.

Description

technical field [0001] The invention relates to a method for preparing a gene-edited COVID-19 vaccine vector, which belongs to the infectious disease prevention and control technology in the field of biomedicine. Background technique [0002] The novel coronavirus (SARS-CoV-2) has posed a serious threat to global public health. The main structure of SARS-CoV-2 includes single-stranded positive-strand nucleic acid (ssRNA), spike protein (S), membrane protein (M), envelope protein (E) and nucleocapsid protein (N). The S protein is cleaved by the host protease into the N-terminal S1 subunit and the C-terminal S2 subunit during the infection process. The S1 subunit is composed of an N-terminal domain (S1-NTD) and a receptor binding domain (S1-RBD). -RBD is responsible for recognizing and binding the host cell surface receptor angiotensin-converting enzyme 2 (ACE2), and the S2 subunit mediates the fusion between the viral envelope and the host cell membrane, allowing the virus t...

AI Technical Summary

Problems solved by technology

It has been reported in the literature that SARS-CoV can replicate in ACE2-transfected 293T cells but not mock-transfected 293T cells

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