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HACE2 knocked-out RNA interference stem cell vector novel coronavirus vaccine

A technology of RNA interference and carrier vaccines, applied in the field of biomedicine, to achieve the effect of safe use

Inactive Publication Date: 2021-04-09
翁炳焕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of a new coronavirus vaccine using artificially constructed stem cells with RNA interference function as a carrier

Method used

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  • HACE2 knocked-out RNA interference stem cell vector novel coronavirus vaccine
  • HACE2 knocked-out RNA interference stem cell vector novel coronavirus vaccine
  • HACE2 knocked-out RNA interference stem cell vector novel coronavirus vaccine

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Embodiment Construction

[0031] The specific implementation methods of the present invention will be described in detail below in conjunction with the accompanying drawings, but these exemplary descriptions do not constitute any limitation to the scope of protection defined by the claims of the present invention.

[0032] 1. Collection of Mesenchymal Stem Cells

[0033] Mesenchymal stem cells (MSCs) are derived from the mesoderm in the early stage of development and belong to pluripotent stem cells, which can be isolated from amniotic fluid cells, amniotic membrane tissue, bone marrow cells, umbilical cord blood, umbilical cord tissue or placental tissue left after the experiment or preserved in a stem cell bank get.

[0034] 1.1. Collection of amniotic fluid mesenchymal stem cells

[0035] Mesenchymal stem cells were isolated from amniocytes remaining after daily prenatal diagnosis in each unit. Amniotic fluid cells are the exfoliated cells of the respiratory system, digestive system and other tiss...

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Abstract

The invention relates to an hACE2 knocked-out RNA interference stem cell vector novel coronavirus vaccine, which is characterized in that an adenovirus vector of a traditional novel coronavirus vaccine is replaced by an hACE2 gene knocked-out RNA interference stem cell. The RNA interference stem cell is a stem cell obtained by recombining targeted interference gene shRNA of new coronavirus M, N, E and / or S genes on the basis of knocking out a susceptibility gene ACE2 of the new coronavirus and transfecting an immortalized gene SV40LT and / or hTERT. The RNA interference stem cell has the functions of resisting new coronavirus infection, infinite passage and inhibiting new coronavirus replication in the stem cell, can be pre-stored in a -196 DEG C stem cell bank for a long time according to names, ABO blood types or HLA types for later use, can be used for individualized treatment of COVID-19 by taking homologous stem cells, and can replace an immunogenic adenovirus vector; a new coronavirus antibody generation gene S1-RBD is inserted into RNA interference stem cell DNA to prepare an RNA interference stem cell vector vaccine capable of generating a new coronavirus neutralizing antibody.

Description

technical field [0001] The invention relates to a hACE2 knockout RNA interference stem cell carrier COVID-19 vaccine, which belongs to the infectious disease prevention and control technology in the field of biomedicine. Background technique [0002] The novel coronavirus (SARS-CoV-2) has posed a serious threat to global public health. The main structure of SARS-CoV-2 includes single-stranded positive-strand nucleic acid (ssRNA), spike protein (S), membrane protein (M), envelope protein (E) and nucleocapsid protein (N). Among them, the S protein is cleaved by host proteases into the N-terminal S1 subunit and the C-terminal S2 subunit during the infection process. The S1 subunit is composed of an N-terminal domain (S1-NTD) and a receptor binding domain (S1-RBD). -RBD is responsible for recognizing and binding the host cell surface receptor angiotensin-converting enzyme 2 (ACE2), and the S2 subunit mediates the fusion between the viral envelope and the host cell membrane, all...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61P31/14C12N15/867C12N15/90C12N15/66C12N15/57C12N15/113C12N15/50C12N5/10
CPCA61K39/12A61K2039/5156A61K2039/53A61P31/14C07K14/005C12N5/0668C12N9/485C12N15/1131C12N15/66C12N15/86C12N15/907C12N2310/14C12N2320/32C12N2510/04C12N2740/15043C12N2770/20022C12N2770/20034C12N2800/107C12Y304/17023
Inventor 翁炳焕
Owner 翁炳焕
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