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A greening bacteria agent for increasing the chlorophyll content of green leafy vegetables and its preparation method and application

A technology of chlorophyll content and green leafy vegetables, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of not being able to tolerate soil microanaerobic environment, not being able to tolerate low temperature, and having no antibacterial effect Disease resistance and other issues

Active Publication Date: 2021-09-14
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, among the microorganisms used in conjunction with organic fertilizers to increase the chlorophyll of green leafy vegetables, most microorganisms cannot produce enzymes, cannot tolerate the low temperature of 18-20°C in the soil, cannot germinate and proliferate in the soil, and cannot tolerate the soil. Oxygen environment, no antibacterial and disease-resistant effects, or no nitrogen-releasing, phosphorus-, and potassium-releasing effects, which lead to unsatisfactory greening effects

Method used

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  • A greening bacteria agent for increasing the chlorophyll content of green leafy vegetables and its preparation method and application
  • A greening bacteria agent for increasing the chlorophyll content of green leafy vegetables and its preparation method and application
  • A greening bacteria agent for increasing the chlorophyll content of green leafy vegetables and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Isolation, screening and identification of Bacillus filamentous bacteria GBW-F006

[0043] 1. Isolation and Screening of Filamentous Bacillus GBW-F006

[0044]Crop rhizosphere soils were taken from perennial vegetable greenhouses in Cangzhou, Handan, Henan, Puyang, Anyang, Qingdao, Haiyang, Qingzhou, and Shouguang in Hebei, stored in a medical test sample bag at 4°C, and separated in time. 56 kinds of crops including pepper, zucchini, cauliflower, melon, tomato and cucumber. Take 2g of soil sample, put it into 18mL sterile water, shake and extract at 30°C and 200rpm / min for 30min, put the sample solution in a water bath at 75°C for 20min, and then carry out gradient dilution, take 100μl of each gradient dilution solution and spread it on the NA plate. Incubate the coated plate upside down at 37°C for 48 hours, pick out single colonies of different shapes with sterilized bamboo sticks, transfer them to the NA slope, culture at 37°C for 48 hours, and confirm the spore...

Embodiment 2

[0075] The preparation method of the chlorophyll agent for improving the chlorophyll content of green leafy vegetables according to the present invention is as follows:

[0076] 1. Activate and cultivate Bacillus filamentous bacteria GBW-F006, Bacillus endophyta GBW-F008 and Clostridium butyricum GBW-N1 respectively in the corresponding seed culture solution to prepare seed solution; wherein,

[0077] The formula of the Bacillus filamentous bacteria GBW-F006 seed culture solution is: 1-1.5g corn steep liquor dry powder, 2-2.5g cottonseed protein powder, 0.8-1g peptone, 0.5-0.8g yeast extract, KH 2 PO 4 0.1~0.3g, MgSO4 7H 2 O 0.05~0.08g, MnSO 4 ·H 2 O 0.03~0.05g neutral protease 50,000 U / g 0.1~0.2g, pH 7.3~7.5, distilled water 1L, culture time 12-14h, culture temperature 35℃~38℃;

[0078] The formula of Bacillus intraplanta GBW-F008 seed culture solution is: corn starch 8-10g, bean cake powder 2-3g, K 2 HPO 4 2~3g, MgSO4·7H 2 O 0.5~0.8g, MnSO 4 ·H 2 O 0.5~0.8g, CaCO ...

Embodiment 3

[0085] The experiment was carried out in a spinach planting base in Loude Town, Xintai City. The spinach to be tested was divided into 6 groups, and the specific groupings are shown in Table 1. The test was set as A0, A1, A2, A3, A4, and A5 in the order of gradual increase in the amount of bacteria diluted with the chlorotic agent, and each group was divided into 6 groups. Blank sterilized expansion culture solution Dilute the greening bacteria agent prepared in Example 2 to a specific amount of bacteria, and then apply it to the root of spinach at 15cm to 20cm with the organic fertilizer base fertilizer at an application dose of 2kg / mu. The scale of each test group was 3 ridges, and 60-80 spinach trees were planted in each ridge.

[0086] Table 1: Grouping of bacteria in different dilutions of greening agent

[0087]

[0088] The experiment lasted for 2 months, focusing on the effects of different dilutions of chlorophyll agent on available nitrogen in spinach rhizosphere...

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Abstract

The invention provides a greening bacteria agent for increasing the chlorophyll content of green leafy vegetables, a preparation method and application thereof. The greening bacteria agent is made by expanding the mixed bacterial seed liquid, including Bacillus filamentous GBW-F006 with preservation number CGMCC No.20918 and Bacillus endophyta GBW-F008 with CGMCC No.20919 and CGMCC No.14499 Clostridium butyricum GBW-N1 prepared from the seed solution. The method of using the greening bacteria agent is simple, only need to dilute the greening bacteria agent and mix it with organic fertilizer in an amount of 2-4kg / mu, and then apply it to the 15-25cm of the root of the green leafy vegetable. The ability of the rhizosphere soil available nitrogen, leaf nitrogen content and chlorophyll content of green leafy vegetables can improve the photosynthesis of green leafy vegetables, promote the growth and quality of green leafy vegetables, and is also beneficial to the planting of green leafy vegetables.

Description

technical field [0001] The invention belongs to the field of plant planting, and in particular relates to a greening bacterium agent for increasing the chlorophyll content of green leafy vegetables, a preparation method and application thereof. Background technique [0002] Green leafy vegetables are a class of fast-growing vegetables that mainly produce fresh green leaves and petioles. There are more than 30 species in more than 10 families in my country. Leaf chlorophyll is an essential substance for the accumulation of organic matter in vegetables through photosynthesis, and its content has an important indicator effect on the absorption of plant nutrients. The higher the chlorophyll content, the stronger the photosynthetic ability, the darker green leaves of the plant will grow stronger, and the resistance to some plant diseases will also increase accordingly. Nitrogen content affects photosynthetic links such as chlorophyll content and photosynthetic rate. Nitrogen def...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/22A01N63/20A01P1/00A01P3/00A01P21/00C05G3/00C05G3/60C05G3/80C09K101/00C12R1/07C12R1/145
CPCA01N63/20A01N63/22C05G3/00C09K17/14C09K2101/00C12N1/20C05G3/60C05G3/80
Inventor 李慧芬张宗国魏秉培张大伟冯海霞陈四明樊连梅滕乐邦
Owner QINGDAO SHANGDE BIOTECH
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