Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Yemazhui sesquiterpene lactone b in the preparation of anti-liver cancer drugs and an anti-liver cancer drug

A sesquiterpene lactone, anti-cancer technology, applied in the field of medicine, can solve the problems of less research and mechanism research, and achieve the effect of increasing membrane density

Active Publication Date: 2022-03-08
CHONGQING THREE GORGES MEDICAL COLLEGE
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on Yemachai in liver cancer and its mechanism

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Yemazhui sesquiterpene lactone b in the preparation of anti-liver cancer drugs and an anti-liver cancer drug
  • Application of Yemazhui sesquiterpene lactone b in the preparation of anti-liver cancer drugs and an anti-liver cancer drug
  • Application of Yemazhui sesquiterpene lactone b in the preparation of anti-liver cancer drugs and an anti-liver cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Inhibitory effect and mechanism of Yemazhui sesquiterpene lactone B on the growth of liver cancer cell lines

[0034] By the CCK8 method, two human liver cancer cells, SMMC-7721 and HCCLM3, were cultured to a density of about 90%, and the cells were counted. They were inoculated in 96-well culture plates according to the experimental groups, and the number of cells in each well was 5×10. 3 6 replicate wells for each group, placed at 37°C, 5% CO after inoculation 2 Cultured in the incubator for 24 hours respectively. In the treatment group, the final concentration of 6, 12, and 24 μmol / L of Yemachai sesquiterpene lactone B was added, and after 24 hours, 48 ​​hours, and 72 hours of incubation, 10 μL of CCK8 solution was added, and incubated in a 37°C incubator for 4 hours. Measure its OD value at 450nm on the microplate reader, the result is as follows figure 1 as shown, figure 1 Middle A is the test result of Yemazhui sesquiterpene lactone B on growth inhibition of SM...

Embodiment 2

[0040] Study on the effect and mechanism of Yemazhui sesquiterpene lactone B on the migration ability of liver cancer cell lines

[0041] Two human liver cancer cells, SMMC-7721 and HCCLM3, in the logarithmic growth phase were inoculated in 6-well plates, 5×10 5 cells / well, 2 mL medium / well. Put in 37℃, 5% CO 2 Incubate for 24 h in a constant temperature incubator. The cells cover the bottom wall, use the tip of a 20 μL pipette to evenly streak the bottom of the culture dish, wash the cells 3 times with PBS, and treat them with serum-free medium containing 6 μM, 12 μM, and 24 μM Yemazi sesquiterpene lactone B respectively For the cells in the experimental group, the cells in the control group were treated with serum-free medium containing 0.1% DMSO. Use an inverted microscope to take pictures at time points of 0, 6, 24, and 48 hours, and use Image J statistical data to calculate the scratch wound healing rate = (0h scratch width - scratch width after culture) / 0h scratch wid...

Embodiment 3

[0045] Study on the mechanism of Yemazhui sesquiterpene lactone B-induced ferroptosis signaling pathway in liver cancer cells

[0046] (1) Yemazhui sesquiterpene lactone B down-regulates the expression of ferroptosis-related protein GPX4 in liver cancer cells

[0047] Two human liver cancer cells, SMMC-7721 and HCCLM3, were cultured. Experimental groups were: control group (DMSO), Yemazhui sesquiterpene lactone B high-dose group (24μM), treated with drugs for 48 hours, and the two cells were in logarithmic growth During the period, discard the culture medium and proceed as follows: wash twice with PBS, add RIPA, phosphatase inhibitors, and protease inhibitors (100:10:1), scrape off the adherent cells with a cell scraper, and suck them into In a centrifuge tube, centrifuge at 3000rpm at 4°C for 5min. The supernatant was collected after centrifugation, and the whole process was completed on ice. The supernatant was collected to extract the total protein; the protein concentrati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the application of Yemazhui sesquiterpene lactone B in the preparation of anti-liver cancer drugs and an anti-liver cancer drug, belonging to the technical field of medicine. Yemazhui sesquiterpene lactone B has a certain inhibitory effect on human liver cancer in vivo and in vitro, and can significantly inhibit the growth of liver cancer cells. The specific mechanism is to arrest the cell cycle in the S phase, down-regulate the cycle-related proteins CDK2 and Cyclin E1 expression. Yemazhui sesquiterpene lactone B inhibits the migration of liver cancer cells, and the specific mechanism is to down-regulate the expression of Vimentin and up-regulate the expression of E‑cadherin. At the same time, Yemazhui sesquiterpene lactone B shrinks the mitochondria of liver cancer cells and increases the membrane density, which can increase the reactive oxygen species of liver cancer cells and induce the signaling pathway of ferroptosis, thus playing an anti-hepatic effect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Yemazhui sesquiterpene lactone B in the preparation of anti-liver cancer drugs and an anti-liver cancer drug. Background technique [0002] Primary liver cancer is one of the common clinical malignant tumors. According to histological analysis, most liver cancers (75-85%) are primary liver cancers. The high morbidity and mortality of liver cancer have brought a huge economic burden to the society. Therefore, exploring effective treatment options for liver cancer is still an urgent clinical problem to be solved. [0003] At present, the treatment options for liver cancer mainly include surgical treatment and non-surgical treatment. Although the most effective method for the treatment of primary liver cancer is surgical treatment, due to the insidious onset of primary liver cancer, asymptomatic or insignificant symptoms in the early stage, most pati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P35/00
CPCA61K31/365A61P35/00
Inventor 张永慧魏勇陈地龙刘桂元张昊洋牟金阿哥韩美月潘李安曹志豪王婷婷董凤罗雾晶余明洁刘欢李佳鑫金俐杉丁文轩杨婉龙刘丹刘奉
Owner CHONGQING THREE GORGES MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com