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Therapeutic milk-borne composite probiotics as well as preparation method and application thereof

A compound probiotic and milk-derived technology, applied in biochemical equipment and methods, microbial-based methods, bacteria, etc., to achieve the effect of improving acid resistance

Inactive Publication Date: 2021-02-19
安徽燕婉健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a therapeutic milk-derived compound probiotic and its preparation method and application, which solves the problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The invention provides a technical solution: a therapeutic milk-derived compound probiotic and its preparation method and application, which are characterized in that the preparation method is: strain inoculation culture, seed tank culture, production culture, adding carrier, drying, Screening, quality inspection, and completion of probiotics include the following steps:

[0024] Select Lactobacillus acidophilus and Bifidobacterium species that were freeze-dried and stored, and place them in two MRS culture dishes at a concentration of 1% for anaerobic culture, and the culture time is 24 hours; Extract part of the cultured Lactobacillus acidophilus and Bifidobacterium species and put them into the seed tank for activation. The oxygen concentration inside the seed tank is maintained at 10%, and the culture time is maintained for 12 hours; the Lactobacillus acidophilus species and Bifidobacterium species after the initial culture Bacillus species, mixed with skim milk ste...

Embodiment 2

[0027] The invention provides a technical solution: a therapeutic milk-derived compound probiotic and its preparation method and application, which are characterized in that the preparation method is: strain inoculation culture, seed tank culture, production culture, adding carrier, drying, Screening, quality inspection, and completion of probiotics include the following steps:

[0028] Select Lactobacillus acidophilus and Bifidobacterium species that were freeze-dried and stored, and place them in two MRS culture dishes at a concentration of 1% for anaerobic culture, and the culture time is 24 hours; After extracting part of the cultured Lactobacillus acidophilus and Bifidobacterium species, put them into the seed tank for activation, the oxygen concentration inside the seed tank was maintained at 13%, and the culture time was maintained for 18 hours; the Lactobacillus acidophilus species and Bifidobacterium species Bacillus species, mixed with 3% inoculum amount of skim milk...

Embodiment 3

[0031] The invention provides a technical solution: a therapeutic milk-derived compound probiotic and its preparation method and application, which are characterized in that the preparation method is: strain inoculation culture, seed tank culture, production culture, adding carrier, drying, Screening, quality inspection, and completion of probiotics include the following steps:

[0032] Select Lactobacillus acidophilus and Bifidobacterium species that were freeze-dried and stored, and place them in two MRS culture dishes at a concentration of 1% for anaerobic culture, and the culture time is 24 hours; Extract part of the cultured Lactobacillus acidophilus and Bifidobacterium species and put them into the seed tank for activation. The oxygen concentration inside the seed tank is maintained at 15%, and the culture time is maintained for 24 hours; the Lactobacillus acidophilus species and Bifidobacterium species Bacillus species, mixed with skim milk sterilized at 115°C for 15 mi...

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PUM

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Abstract

The invention discloses therapeutic milk-borne composite probiotics as well as a preparation method and application thereof. The preparation method is characterized by comprising the following steps:inoculating and culturing strains, culturing in a seeding tank, performing producing and culturing, adding a carrier, performing drying, performing sieving and performing quality inspection to obtainthe probiotics. Lactobacillus acidophilus strains and bifidobacterium strains, having the concentration of 1%, are respectively placed in two MRS culture dishes for anaerobic culture. According to thetherapeutic milk-borne composite probiotics as well as the preparation method and the application thereof, the seed tank is used for fully activating lactobacillus acidophilus and bifidobacteria to provide certain nutrients for the lactobacillus acidophilus and the bifidobacteria, and the lactobacillus acidophilus and the bifidobacteria are centrifuged, cleaned and frozen, so that a good effect of mixing the two bacteria is guaranteed, and the situation that after freeze storage, thalli can be reused and propagated can be guaranteed; and the thalli are embedded with calcium alginate to form composite probiotic particles, and the acid resistance of the composite probiotics is improved under the condition of no influence on the bile salt tolerance of the probiotics.

Description

technical field [0001] The invention relates to the technical field of probiotic preparation, in particular to a therapeutic milk-derived compound probiotic and its preparation method and application. Background technique [0002] Probiotics can regulate the normal flora of the gastrointestinal tract of the body, maintain micro-ecological balance, improve food digestibility and biological value, lower serum cholesterol, control endotoxins, inhibit the growth and reproduction of spoilage bacteria in the intestine and the production of spoilage products, and produce nutrients. Stimulate tissue development, thereby exerting effects on the body's nutritional status, physiological functions, cell infection, drug effects, toxic reactions, immune responses, tumorigenesis, aging process, and sudden emergency responses. [0003] Commonly used probiotics are usually produced with a single species of bacteria. The acid resistance of this type of probiotics needs to be improved, and the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12N11/10C12N11/04A61K35/747A61K35/745A61K9/16A61P1/14C12R1/23C12R1/01
CPCC12N1/20C12N1/04C12N11/10C12N11/04A61K35/747A61K35/745A61K9/1652A61P1/14A61K2300/00
Inventor 张正前
Owner 安徽燕婉健康科技有限公司
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