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Soil microorganism prebiotic composition capable of improving plant system resistance and application thereof

A technology of soil microorganisms and prebiotics, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as soil microbial community imbalance, decline in the abundance of beneficial bacteria, and difficulty in survival after addition of exogenous beneficial bacteria, to achieve The effect of reducing the cost of production raw materials, improving the quality of agricultural products, and reducing the incidence of crop disease

Active Publication Date: 2021-02-19
JIANGSU GUOXIN UNION ENERGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to develop a rhizosphere probiotic that increases the abundance of Pseudomonas in soil to improve plant disease resistance in view of the problems of imbalanced soil microbial community, decreased abundance of beneficial bacteria, and difficulty in survival after addition of exogenous beneficial bacteria. meta recipe

Method used

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  • Soil microorganism prebiotic composition capable of improving plant system resistance and application thereof
  • Soil microorganism prebiotic composition capable of improving plant system resistance and application thereof
  • Soil microorganism prebiotic composition capable of improving plant system resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Obtaining of prebiotic formula

[0033] Pathogens were inoculated into Arabidopsis leaves for infection culture. The metabolome of root exudates from healthy and pathogen-infected plants was determined one week later. It was found that after inoculation of pathogenic bacteria, capric acid, dodecanoic acid, tridecanoic acid, pentadecanoic acid, palmitoleic acid, hexadecanoic acid, octadecanoic acid, arachidic acid, isoleucine, leucine, etc. amino acid, methionine, proline, tryptophan, ornithine, aspartic acid, alanine, oxaloacetate, citric acid, cis-aconitic acid, ketoglutaric acid, succinic acid, malic acid, maltose , ribose, glucose, sucrose, fructose and xylose, a total of 28 substances were different. After subdivision, it was found that these 28 substances belonged to 4 categories, namely, long-chain fatty acids (capric acid, dodecanoic acid, tridecanoic acid, pentadecanoic acid, palmitoleic acid, hexadecanoic acid, octadecanoic acid and arachidic a...

Embodiment 2

[0035] Embodiment 2. Further optimization of prebiotic formula

[0036] Any one or more of 8 kinds of amino acids and any one or more of 8 kinds of fatty acids are combined in the solution and added to the soil according to the above requirements. The results show that any one of the above-mentioned amino acids combined with any of the above-mentioned fatty acids can increase the abundance of soil Pseudomonas. When the types of amino acids and fatty acids contained in prebiotics reach 5 or more types (including 5 types) respectively, their effects are not significantly different from those of all 16 substances, such as figure 2 Shown are three preferred formulas that comprise more than 10 kinds of substances (three formulas are shown in Table 1) and the formulas of all 16 kinds of substances are on the promotion of Pseudomonas abundance in soil.

[0037] Table 1 Three preferred prebiotic formulations

[0038]

Embodiment 3

[0039] Example 3. Use of soil after prebiotics to help plants resist the invasion of pathogenic bacteria

[0040] 3.1 The soil after the application of prebiotics helps Arabidopsis resist the invasion of aboveground pathogens

[0041] The Arabidopsis thaliana that had been reared in advance were planted in the soil after applying the above-mentioned several prebiotic formulas, and the leaves of Arabidopsis were inoculated with pathogenic bacteria two weeks later. The incidence was calculated 7 days after inoculation. The result is as image 3 As shown, the disease incidence rate of plants was about 20% in the soil where 4 kinds of prebiotics were applied, while the disease incidence rate of the control treatment without applying the product was above 60%. It shows that the product can help plants resist the invasion of pathogenic bacteria and significantly reduce the incidence of disease.

[0042] 3.2 Isolation of Pseudomonas in soil after application of prebiotics

[0043] ...

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Abstract

The invention discloses a soil microorganism prebiotic composition for improving plant system resistance and application thereof. The soil microorganism prebiotic composition is prepared from capric acid, dodecanoic acid, tridecanoic acid, pentadecanoic acid, palmitoleic acid, hexadecanoic acid, octadecanoic acid, arachidonic acid, isoleucine, leucine, methionine, proline, tryptophan, ornithine, alanine and aspartic acid. After application, the number of pseudomonas in soil can be increased by more than two orders of magnitudes, the plant system resistance is remarkably improved, and the morbidity is reduced. The formula product is suitable for herbaceous crops such as fruits and vegetables.

Description

[0001] Case Description [0002] The present invention is a divisional application of a Chinese invention patent application with the application number 2019106007488, the application date is 2019-07-04, and the title of the invention is: a prebiotic formula product that regulates soil microorganisms to increase plant system resistance and reduce disease incidence. technical field [0003] The invention belongs to the technical field of agricultural chemicals, and relates to a soil microbial community conditioner and its application method. Background technique [0004] The planting area of ​​economic crops in our country is increasing year by year. While continuous cropping and intensive planting bring considerable economic value, they also bring a heavy load to the soil. The most notable ones are frequent soil continuous cropping obstacles, serious soil-borne diseases, and difficulty in surviving exogenous beneficial bacteria. The root cause is the imbalance of soil microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/02A01N37/44A01N43/36A01N43/38A01P21/00A01B79/00
CPCA01B79/00A01N37/02A01N37/44A01N43/36A01N43/38
Inventor 袁军文涛沈其荣
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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