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Culture method of artificial cordyceps militaris fruiting body

A cultivation method and technology of Cordyceps militaris are applied in the field of biomedicine, can solve the problems of inability to take into account high-yield artificial Cordyceps militaris fruiting bodies, neglect the content of cordycepin, etc., achieve anti-fatigue, regulate the heart and lungs, improve product quality, and achieve high cordycepin content. Effect

Pending Publication Date: 2021-02-05
SHANGHAI GUOBAO ENTERPRISE DEV CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the prior art often ignores the improvement of the content of cordycepin in Cordyceps militaris, and it is also impossible to take into account the artificial Cordyceps militaris fruiting body and its four elements of high yield, high cordycepin content, high Cordyceps polysaccharide content and high adenosine content. training method

Method used

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  • Culture method of artificial cordyceps militaris fruiting body
  • Culture method of artificial cordyceps militaris fruiting body
  • Culture method of artificial cordyceps militaris fruiting body

Examples

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Embodiment 1

[0032] A kind of culture method of artificial Cordyceps militaris fruiting body, the method comprises the following steps:

[0033] (1) Preparation of culture medium: under 10,000-level purification conditions, each box of culture medium includes 750g of rice, 30g of soybean powder, 950ml of water, 75ml of tomato juice and 1g of inorganic salt, and the pressure is 0.1- 0.2MPa, 35-40min damp heat sterilization, to form a culture medium; rice mainly provides carbon source, soybean powder mainly provides nitrogen source, inorganic salt provides trace elements; inorganic salt is dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate, The ratio of the mass concentration of dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate is (2-3):(0.2-0.4):(0.03-0.05); in this step operation, sterilization can also be sprayed with 0.2% bromogeramine solution, Turn on the ultraviolet lamp for disinfection for 30 minutes before and after the preparation operati...

Embodiment 2

[0065] The difference from Example 1 is that the wavelength of light source for light cultivation is 400-750nm.

Embodiment 3

[0067] The difference from Example 1 is that the wavelength of the light source for light cultivation is 530-550 nm.

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Abstract

The invention relates to a culture method of an artificial cordyceps militaris fruiting body. The culture method comprises the following steps of (1), culture medium preparation, specifically, mixingrice, soybean powder, water, tomato juice and an inorganic salt, and carrying out moist heat sterilization to form a culture medium; (2), hypha inoculation; (3), dark culture; (4), illumination culture, specifically, after white hyphae grow on the culture medium subjected to dark culture, putting a culture room under an illumination condition for culture, and gradually generating the cordyceps militaris fruiting body; and (5), cordyceps militaris fruiting body collection, specifically, collecting the cultured cordyceps militaris fruiting body. Compared with the prior art, the cordyceps militaris fruiting body cultured by the method is high in yield, cordycepin content, cordyceps polysaccharide content and adenosine content, and then the cordyceps militaris fruiting body has huge value in the fields of tumor resistance, bacteria resistance, virus resistance, anoxia resistance, fatigue resistance, cardiopulmonary regulation and the like.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for cultivating artificial fruit bodies of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, is a complex of insects and fungi formed by fungi parasitic on insects such as Lepidoptera. It has various medicinal effects such as anti-tumor, anti-inflammation, hypoxia resistance, and anti-fatigue. It has been widely used as a medicinal fungus in various regions. [0003] Cordycepin is a characteristic component of some fungi of the genus Cordyceps, and it is also one of the most important active ingredients in fungi of the genus Cordyceps. It has medicinal functions such as immune regulation, anti-tumor, anti-fatigue, and heart and lung regulation. As a new type of broad-spectrum antibiotic, cordycepin can inhibit the RNA synthesis of viruses with its unique antibacterial and antiviral activity; it h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/30
CPCA01G18/20A01G18/30A01G18/00
Inventor 唐永范唐培宸
Owner SHANGHAI GUOBAO ENTERPRISE DEV CENT
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