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COVID-19 IgG/IgM detection kit, detection card, rare earth nanoprobe and preparation method thereof

A COVID-19, nanoprobe technology, applied in the field of COVID-19 IgG/IgM detection kits, detection cards, rare earth nanoprobes and preparations, can solve the problem of rapid diagnosis and screening of patients with unfavorable pneumonia, and detection time of viral nucleic acid It can eliminate the background fluorescence interference, eliminate the excitation light interference, and achieve the effect of high sensitivity and high sensitivity.

Pending Publication Date: 2021-01-05
厦门奥德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nucleic acid detection is currently the "gold standard" for the diagnosis of new coronavirus infection. However, virus nucleic acid detection has a series of problems such as long detection time and cycle, high environmental requirements, high technical force requirements, high hardware equipment requirements, and high price. Provincial / municipal CDCs and larger tertiary hospitals are not conducive to the rapid diagnosis and screening of pneumonia patients, and are not conducive to the prevention and control of the epidemic

Method used

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  • COVID-19 IgG/IgM detection kit, detection card, rare earth nanoprobe and preparation method thereof
  • COVID-19 IgG/IgM detection kit, detection card, rare earth nanoprobe and preparation method thereof
  • COVID-19 IgG/IgM detection kit, detection card, rare earth nanoprobe and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0058] The rare earth nanoprobe of this embodiment is praseodymium-doped gadolinium-lutetium fluoride-coated sodium yttrium fluoride with a core-shell structure, the particle size is 40nm-60nm, and its composition is:

[0059] NaGdxLu1-y-xF4:yPr@NaYF4, where NaGdxLu1-xF4 is the host and the dopant ion is Pr 3+ The colon ":" represents praseodymium doping; x and y are rare earth ion doping mole percentages, and the range of x is 20% to 90%, preferably 40%; the range of y is 1% to 10%, preferably 5 %; NaYF4 is the shell layer, @ indicates that NaYF4 is coated on the surface of NaGdxLu1-y-xF4:yPr.

[0060] Further, the rare earth nanoprobe is stable in the ground state, and emits fluorescence with a wavelength in the range of 450-700 nm under the action of an excitation light source of 365 nm. NaGd x Lu 1-y-x f 4 :yPr@NaYF 4 Fluorescence spectra of rare earth nanoprobes under excitation at 365nm, such as figure 2 shown. NaGd x Lu 1-y-x f 4 :yPr@NaYF 4 TEM images of ra...

Embodiment 2

[0066] Such as figure 1 As shown, the COVID-19 IgM / IgG detection card of the present embodiment includes:

[0067]Substrate 1; as the substrate of the COVID-19 IgM / IgG detection card;

[0068] Coating film 4; arranged in the middle of the upper surface of the substrate 1;

[0069] Bonding pad 3: overlapped at one end of the upper surface of the coating film 4

[0070] Sample pad 2; overlapped with one end of the upper surface of the binding pad 3;

[0071] Absorbent paper 4; overlapped on the other end of the upper surface of the coating film 3;

[0072] Wherein, the binding pad 3 is sprayed with a microsphere thread 31, and the microsphere thread 31 is the COVID-19 recombinant antigen labeled with the rare earth nanoprobe provided in Example 1; the coating film 4 is sequentially provided with a detection line M 41. The detection line G 42 and the quality control line 43, the detection line 41 is close to the binding pad 3, the detection line M 41, the detection line G 42 ...

Embodiment 3

[0084] The COVID-19 IgM / IgG detection kit of the present embodiment includes:

[0085] COVID-19 IgM / IgG detection card: adopt the COVID-19 IgM / IgG detection card provided by embodiment 2;

[0086] ID card containing the calibration curve: the calibrators with different antibody titers determined by the COVID-19 IgM / IgG test card, take the reciprocal of the titer of the calibrators as the abscissa, and the fluorescence signal ratio as the ordinate, draw a standard curve, write Enter and generate the corresponding two-dimensional code information stored in the ID card. The corresponding QR code information on the reagent card can be read and the corresponding antibody titer can be measured by the dry fluorescent immunoassay analyzer.

[0087] The method for the quantitative detection of COVID-19 by the COVID-19 IgM / IgG detection kit of the present embodiment may further comprise the steps:

[0088] Step 1: Serum / plasma / whole blood samples are used as test samples;

[0089] St...

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Abstract

The invention discloses a COVID-19 IgG / IgM detection kit, a detection card, a rare earth nanoprobe and a preparation method thereof, the rare earth nanoprobe is praseodymium-doped gadolinium lutetiumfluoride coated sodium yttrium fluoride with a core-shell structure, the particle size is 40 nm-60 nm, the rare earth nanoprobe is composed of NaGdxLu1-y-xF4:yPr coated NaYF4, wherein NaGdxLu1-xF4 isa matrix, and the doping ion is Pr<3+>; wherein the colon : means praseodymium doping; wherein x and y are rare earth ion doping mole percentages, x ranges from 20% to 90%, and y ranges from 1% to 10%; wherein NaYF4 is a shell layer, @ is characterized in that NaYF4 is coated on the surface of NaGdxLu1-y-xF4: yPr. The nanoprobe is a rare earth fluoride nanomaterial and has the advantages of beinglow in background, long in light-emitting service life, strong in fluorescence signal, high in signal-to-noise ratio and the like, the probe is connected with a new coronavirus recombinant antigen through a covalent bond, a labeled product is stable, and the nanoprobe has the advantages of being high in sensitivity, high in accuracy, rapid, easy and convenient to detect and the like. Early new coronavirus suspected patients can be screened and detected, and clinical definite diagnosis can be quickly and specifically assisted.

Description

technical field [0001] The invention relates to a COVID-19 IgG / IgM detection kit and its microspheres as well as preparation and detection methods. Background technique [0002] Based on the current epidemiological investigation of the new coronavirus, the incubation period is generally 3-7 days, and the longest is no more than 14 days. The main manifestations of the infected person are fever, fatigue, and dry cough. Upper respiratory symptoms such as nasal congestion and runny nose are rare. About half of the patients developed dyspnea more than a week later, and severe cases rapidly progressed to acute respiratory distress syndrome, septic shock, metabolic acidosis that was difficult to correct, and coagulation dysfunction. It is worth noting that severe and critically ill patients may have moderate to low fever or even no obvious fever during the course of the disease. [0003] The new type of coronavirus is also known as severe acute respiratory syndrome (SARS) corona...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02B82Y20/00B82Y40/00G01N33/533G01N33/543G01N33/558G01N33/569G01N21/64
CPCB82Y20/00B82Y40/00C09K11/02C09K11/7773G01N21/6408G01N21/6428G01N33/533G01N33/54313G01N33/558G01N33/56983G01N2333/165G01N2469/20
Inventor 张云张肖宋良刘宁林文娜吴志滔洪茂椿
Owner 厦门奥德生物科技有限公司
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