6''-O-succinyl daidzin derived from biological method and application of 6''-O-succinyl daidzin derived from biological method to preparation of neuroprotective drugs and health products

A technology of neuroprotection and succinyl, applied in the field of medicine, can solve problems such as limited application, low production efficiency, and difficulty in commercial promotion and application, and achieve significant drug effects

Active Publication Date: 2020-12-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1999, Toda et al. (Biol Pharm Bull, 22(11), 1193-1201) isolated a novel functional soybean isoflavone derivative 6″-O-succinyl daidzein (6″-O -succinyldaidzin), but due to the extremely low content, it is difficult to be applied commercially. In terms of biological activity, the article reports that the flavonoid derivative 6″-O-succinyldaidzin has the effect of anti-osteoporosis
Using the method of bio-fermentation transformation, the acquisition of 6″-O-succinyl daidzein is expected to break through the bottleneck (Journal of Food Science, 2010, 75 (1): C128-33; Biotechnology and Applied Biochemistry, 2015, 62 (2): 255- 259), but due to the low fermentation level, using Bacillus natto or Bacillus licheniformis, the production capacity is lower than 0.015g / L / h, it is difficult to obtain a large amount of 6″-O-succinyl daidzein and conduct pharmacological activity research, thus limiting its Application in the fields of medicine and food
[0004] The method of separating and obtaining soybean isoflavone derivatives from natto usually has problems such as high cost and cumbersome purification steps, and the production efficiency of ordinary bio-fermentation conversion methods is low, and it is difficult to obtain soybeans of 6″-O-succinyl daidzein on a large scale Isoflavone Derivatives

Method used

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  • 6''-O-succinyl daidzin derived from biological method and application of 6''-O-succinyl daidzin derived from biological method to preparation of neuroprotective drugs and health products
  • 6''-O-succinyl daidzin derived from biological method and application of 6''-O-succinyl daidzin derived from biological method to preparation of neuroprotective drugs and health products
  • 6''-O-succinyl daidzin derived from biological method and application of 6''-O-succinyl daidzin derived from biological method to preparation of neuroprotective drugs and health products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]Fermentation of Bacillus amyloliquefaciens FJ18 and preparation of resting cells

[0045] Fermentation of Bacillus amyloliquefaciens FJ18 was inoculated into the seed medium: yeast extract 5.0g / L, peptone 10.0g / L, NaCl 10.0g / L, pH7.0, cultured at 30°C, 200rpm for 12 hours. Expansion medium, fermentation medium, its components and contents are: sucrose 20g / L, yeast powder 15g / L.KH 2 PO 4 1.0g / L, CaCl 2 0.8g / L. The pH was adjusted to 8.0 with NaOH. The seed solution was inoculated into the expansion medium and the fermentation medium at 0.5% (v / v), and cultivated at 30° C. and 200 rpm for 12 hours. After centrifuging at 10000rpm for 15 minutes, the bacterial cells were collected, washed 1-2 times with physiological saline, and the resting cells of Bacillus amyloliquefaciens FJ18 were obtained.

Embodiment 2

[0047] The thalline cell fermentation broth in Example 1 was filtered to obtain wet thalline. The raw material solution, that is, the reaction solution, is prepared with dimethyl sulfoxide, daidzein, sucrose, and phosphate buffer. The ratio of organic solvent dimethyl sulfoxide in the reaction solution is 20% (v / v), daidzein 0.5g / L, the molar concentration of phosphate buffer is 150mmol / L, the pH of phosphate buffer is 8.0, and the sucrose concentration is 50g / L L. Disperse the above-obtained wet bacteria in the reaction solution, add it to the reactor, cultivate it at 30°C and 200rpm for 12 hours, centrifuge at 10000rpm for 10 minutes to obtain the supernatant of the reaction solution, and measure the conversion rate of daidzein by HPLC detection and analysis was 96.2%.

[0048] The product is separated with a macroporous resin, and an appropriate amount of resin is taken, soaked in ethanol for 24 hours, and then resin fragments and sundries are removed. Wet Packing Rins...

Embodiment 3

[0055] The thalline cell fermentation broth in Example 1 was filtered to obtain wet thalline. The raw material solution, that is, the reaction solution, is prepared with dimethyl sulfoxide, daidzein, sucrose, and phosphate buffer. The ratio of organic solvent dimethyl sulfoxide in the reaction solution is 15% (v / v), daidzein 1.0g / L, the molar concentration of phosphate buffer is 125mmol / L, the pH of phosphate buffer is 8.0, and the sucrose concentration is 25g / L L. Disperse the above-obtained wet bacteria in the reaction solution, add it to the reactor, cultivate it at 30°C and 200rpm for 12 hours, centrifuge at 10000rpm for 10 minutes to obtain the supernatant of the reaction solution, and measure the conversion rate of daidzein by HPLC detection and analysis was 95.6%.

[0056] The product is separated with a macroporous resin, and the method is the same as in Example 2; the product detection method is the same as in Example 2.

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Abstract

The invention discloses 6''-O-succinyl daidzin derived from a biological method as shown by a following formula (1), application of the 6''-O-succinyl daidzin derived from the biological method in anaspect of preparation of neuroprotective drugs and health products, and a drug using the 6''-O-succinyl daidzin as an active ingredient, and belongs to the technical field of medicine. The 6''-O-succinyl daidzin provided by the invention, as a rare functional substance in functional food natto, is a soy isoflavone derivative, and has the characteristic of strong neuroprotective effect, and a new choice is provided for the health care product development and the neuroprotective drug development.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the use of 6"-O-succinyl daidzin in the preparation of neuroprotective drugs and health care products. Background technique [0002] Soy isoflavones (Soy isoflavones) are phytochemicals mainly derived from the pods of legumes. Studies have shown that soybeans can prevent cardiovascular diseases, prevent and treat osteoporosis in women, and treat diabetes and kidney disease. There are 12 kinds of soy isoflavones naturally present in soybeans, mainly including daidzein, genistein, glycitein, daidzin, genistin, propanediol Acyl daidzin (6″-O-malonydidzin), malonyl genistin (6″-O-malonygenistin), etc. [0003] Natto (fermented from soybeans and Natto bacteria) is a functional health food. Studies have shown that compared with soybeans, natto has stronger functional health effects, such as thrombolysis, anti-tumor, blood pressure lowering, and antibacterial. Disinfection, adjustment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/60A61K31/7048A61P25/00A23L33/105C12R1/07
CPCC12P19/60A61K31/7048A61P25/00A23L33/105A23V2002/00A23V2200/322A23V2250/21172
Inventor 黄志恒张恩泽黄子争刘佳琪俞子静张森段金廒
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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