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A double network hydrogel shell polycystic core structure Trichoderma harzianum fungus

A technology of Trichoderma harzianum and hydrogel, applied in fungi, microorganisms, microorganism-based methods, etc., can solve the problems of poor heat resistance, restricted Trichoderma harzianum, long germination time, etc.

Active Publication Date: 2021-07-06
江西华威科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the common spore powder has low activity, long germination time, and low germination efficiency, which greatly restricts the efficacy of Trichoderma harzianum.
At the same time, the poor heat resistance and easy to be destroyed by mechanical force of Trichoderma harzianum limit its production form and product form, and affect the subsequent transportation and storage

Method used

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  • A double network hydrogel shell polycystic core structure Trichoderma harzianum fungus
  • A double network hydrogel shell polycystic core structure Trichoderma harzianum fungus
  • A double network hydrogel shell polycystic core structure Trichoderma harzianum fungus

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Embodiment 1

[0050] Embodiment 1 Trichoderma harzianum fungal agent of a kind of double-network hydrogel shell polycystic core structure

[0051] The preparation method comprises the following steps:

[0052] (a) Bacteria core layer: mix Trichoderma harzianum powder and filling powder in a ratio of 1:3 (w / w) (filling powder is bacterial cellulose: soluble starch: calcium carbonate = 1:1:1), Use a pelletizing machine to make a spherical shape with a diameter of 2mm, that is, the bacterial agent core layer;

[0053] (b) protective film layer: in the coating pan, spray one layer of polyvinyl alcohol-containing protective film on the surface of the spherical particles of the fungal agent nuclear layer, and the mass ratio of the bacterial agent nuclear layer to the protective film layer is 5:1; The protective film is 10% polyvinyl alcohol aqueous solution (wherein, polyvinyl alcohol is composed of polyvinyl alcohol with a degree of polymerization of 500 and 1700, and the model ratio is: 05:17=...

Embodiment 2

[0059] Embodiment 2 A Trichoderma harzianum fungal agent of a double-network hydrogel shell polycystic core structure, comprising the following steps:

[0060] (a) Bacteria core layer: Trichoderma harzianum powder and filling powder are mixed evenly in a ratio of 1:1 (w / w) (filling powder is bacterial cellulose: soluble starch: calcium carbonate=1:1:1), Use a pelletizing machine to make a spherical shape with a diameter of 2mm, that is, the bacterial agent core layer;

[0061] (b) protective film layer: in the coating pan, spray one layer of polyvinyl alcohol-containing protective film on the surface of the spherical particles of the fungal agent core layer, and the ratio of the bacterial agent core layer to the protective film layer is 5:1; The film is an aqueous solution of 10% polyvinyl alcohol (the ratio of the type of polyvinyl alcohol used is: 05:17=1:3).

[0062] (c) Nutritional synergistic layer: the nutritional synergist is mixed with the bacterial agent core layer w...

Embodiment 3

[0099] Embodiment 3 Trichoderma harzianum fungal agent of a kind of double-network hydrogel shell polycystic core structure

[0100] The preparation method comprises the following steps:

[0101] (a) Bacteria core layer: mix Trichoderma harzianum and filling powder at a ratio of 3:10 (w / w) (filling powder is bacterial cellulose: soluble starch: calcium carbonate = 0.5:1:0.5), and use The pelletizing machine is made into a spherical shape with a diameter of 2mm, i.e. the bacterial agent core layer; (b) protective film layer: in the coating pan, a layer of polyvinyl alcohol-containing protective film is sprayed on the spherical particle surface of the bacterial powder bacterial agent core layer, and the bacteria The mass ratio of agent core layer to protective film layer is 2:1; the protective film is 20% polyvinyl alcohol aqueous solution (model ratio: 05:17=1:2);

[0102] (c) Nutrient synergistic layer: the nutritional synergist is introduced, mixed with the bacterial agent c...

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Abstract

The invention belongs to the field of microbial bacterial fertilizer, and in particular relates to a trichoderma harzianum fungus with a double-network hydrogel shell and multi-cystic core structure, a preparation method and application thereof. In the present invention, Trichoderma harzianum, bacterial cellulose, soluble starch, calcium carbonate and other powders are evenly mixed to form a bacterial agent core layer; a polyvinyl alcohol film is attached to the surface of the bacterial agent core layer to isolate the bacterial agent core layer from the external environment; Subsequently, the nutritional synergist represented by glucose, ammonium sulfate, potassium dihydrogen phosphate, etc. is coated; finally, a polyvinyl alcohol-glycerol / gelatin double network hydrogel shell is formed on the surface of the nutritional synergistic layer. The method can obtain the Trichoderma harzianum fungus agent with low encapsulation amount of bacterial powder and high bacterial activity.

Description

Technical field: [0001] The invention belongs to the field of microbial bacterial fertilizer, and in particular relates to a trichoderma harzianum fungus with a double-network hydrogel shell and multi-cystic core structure, a preparation method and application thereof. Background technique: [0002] In order to pursue higher crop yields and high-quality agricultural products, traditional fertilizers such as nitrogen fertilizers and phosphorus fertilizers are often used in excess. Excessive accumulation of nitrogen and phosphorus elements will lead to soil pollution or eutrophication of water bodies, which is not conducive to the long-term development of agriculture. In recent years, as an environmentally friendly and pollution-free organic fertilizer, microbial fertilizer has received widespread attention and recognition from the society. The efficacy of microbial fertilizer refers to the degree of beneficial effects on soil or crops caused by the growth and reproduction of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N11/084C12N11/02C12R1/885
CPCC12N1/14C12N11/02C12N11/084
Inventor 邹立强吴通陈贵忠刘伟
Owner 江西华威科技有限公司
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