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Method for preparing agarose

A technology of agarose and colloid, which is applied in the field of preparation of agarose, can solve the problems of high cost, cumbersome production process, and long production cycle, and achieve the effects of reducing intermediate production links, improving desulfurization efficiency, and saving production costs

Active Publication Date: 2020-12-18
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has certain disadvantages in the process of producing agarose. The raw material agar often needs to be extracted from seaweed by traditional alkali treatment process to make agar powder and then further modified. There are problems such as long production cycle, cumbersome production process and high cost.

Method used

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  • Method for preparing agarose
  • Method for preparing agarose
  • Method for preparing agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Different pH

[0059] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0060] Acidification treatment: Add sulfuric acid, oxalic acid and disodium edetate to the Gracilaria after rinsing according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40 minutes, pour off the liquid, soak and rinse until the pH of the washing liquid is neutral.

[0061] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral.

[0062] Boil gelatin: wash the Gracilaria gracilaria, boil t...

Embodiment 2

[0067] Embodiment 2 different hydrogen peroxide additions

[0068] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0069] Acidification treatment: Add sulfuric acid, oxalic acid and disodium edetate to the Gracilaria after rinsing according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40 minutes, pour off the liquid, soak and rinse until the pH of the washing liquid is neutral.

[0070] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral.

[0071] Boil gelatin: wash the G...

Embodiment 3

[0076] Embodiment 3 different reaction times

[0077] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0078] Acidification treatment: Add sulfuric acid, oxalic acid and disodium edetate to the Gracilaria after rinsing according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40 minutes, pour off the liquid, soak and rinse until the pH of the washing liquid is neutral.

[0079] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral.

[0080] Boil gelatin: wash the Gracilaria gra...

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Abstract

The invention relates to a method for preparing agarose. The method comprises the following steps: cleaning gracilaria, and carrying out alkali treatment, acidification treatment, bleaching treatment,gelatin boiling, filtration, cooling and solidification to obtain colloid; stirring and crushing the colloid; and adding water into the crushed colloid, heating, adjusting the pH value, adding an H2O2 solution for reaction, filtering, washing and repeatedly washing until no H2O2 is left in the colloidal particle sample and the pH value of the washing liquid is 7 after the reaction is finished, and freezing, dehydrating, drying and crushing the sample to obtain the agarose. According to the method, the content of sulfuric acid groups of agar can be remarkably reduced, the gel strength is improved, the electroendosmosis is remarkably reduced, the commercially available agarose index requirement is met, the reaction time is short, and the method is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of seaweed deep processing, in particular to a method for preparing agarose. Background technique [0002] Agarose is a polysaccharide polymer and the main component of agar. Its basic structure is repeated alternating 1,3-linked β-D-galactose and 1,4-linked 3,6-anhydro-α-L - Galactose. Agarose is white or slightly yellow powder, odorless and tasteless, and has good solubility in hot water and can also be dissolved in dimethyl sulfoxide (DMSO). Compared with agar, agarose contains less negatively charged groups and has better properties. It has the characteristics of high gel strength, low sulfate content, and low electroosmosis. These properties also make agarose have great potential in the fields of biology and medicine. Wide range of applications. [0003] In the 1960s, a scientist in Sweden isolated agarose for the first time. Since then, researchers from various countries have begun to explore the te...

Claims

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Application Information

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IPC IPC(8): C08B37/12
CPCC08B37/0003C08B37/0039
Inventor 肖安风张聪肖琼陈福泉张永辉杨秋明
Owner SHANGHAI OPM BIOSCI CO LTD
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