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Composite microbial agent and application thereof in regulating and controlling pH value of pit mud

A technology of compound microbial agent and compound microbial agent, which is applied in the field of compound microbial agent and its application in regulating the pH of pit mud, and can solve problems such as interference

Active Publication Date: 2020-11-20
JIANGSU YANGHE BREWERY JOINT STOCK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the pit mud micro-ecosystem has self-healing functions, the optimization of human interference, maintenance of pit mud with sufficient water, balanced nutrition, and appropriate pH value and other pit maintenance measures have a great impact on the construction and maintenance of a good micro-ecological flora.
Currently, chemical addition of Ca(OH) is used in pit mud production 2 , ammonia, etc. to adjust the pH of the artificial pit mud, which did not fundamentally solve the acidification problem of the pit mud

Method used

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  • Composite microbial agent and application thereof in regulating and controlling pH value of pit mud
  • Composite microbial agent and application thereof in regulating and controlling pH value of pit mud
  • Composite microbial agent and application thereof in regulating and controlling pH value of pit mud

Examples

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preparation example Construction

[0050] The preparation method of the seed liquid involved in the following examples is as follows:

[0051] Preparation method of lactic acid-utilizing bacteria seed solution: inoculate single colonies of Bacillus cereus CCTCC AB 205244 and Bacillus subtilis subsp. The seed liquid of Bacillus cereus CCTCC AB 205244 and the seed liquid of Bacillus subtilis subsp. subtilis CICC 20855 were obtained.

[0052] The preparation method of acetic acid-utilizing bacteria seed solution: inoculate single colonies of Bacillus cereus CCTCC AB 205244, Bacillus thiaminolyticum CGMCC 1.3475, Bacillus lysinica CICC 20021, and Brevibacillus soil CCTCC AB95009 into the In sodium acetate liquid medium, 37 ℃ high-level liquid static culture for 5 days, respectively to obtain Bacillus cereus CCTCC AB 205244 seed solution, Bacillus meelovii CGMCC 1.3475 seed solution, Bacillus lysinus CICC 20021 seed solution Liquid, Brevibacillus soil CCTCC AB 95009 seed liquid.

[0053] The preparation method of ...

Embodiment 1

[0056] Embodiment 1: the preparation of composite microbial bacterial agent

[0057] Specific steps are as follows:

[0058] 1. Preparation of composite bacterial solution,

[0059] (1) Preparation of compound lactic acid-utilizing bacteria: inoculate Bacillus cereus CCTCC AB 205244 and Bacillus subtilis subsp. 37°C high-level liquid static culture for 5-7 days until the number of microscopic bacteria is 2.0×10 10 Individual / mL, obtain the compound lactic acid utilization bacteria bacterium liquid; Wherein, described Bacillus cereus CCTCC AB 205244, Bacillus subtilis subsp. The total inoculum amount of Bacillus CCTCC AB 205244 seed liquid and Bacillus subtilis subsp. subsp. CICC 20855 seed liquid in the culture medium was 10% (v / v).

[0060] (2) Preparation of compound acetic acid-utilizing bacteria liquid: Bacillus cereus CCTCC AB 205244, Bacillus thiaminolyticum CGMCC 1.3475, Bacillus lysinus CICC 20021, and Brevibacillus agricultivum CCTCC AB95009 were prepared according...

Embodiment 2

[0070] Example 2: Application of compound microbial agent in regulating pit mud pH

[0071] Specific steps are as follows:

[0072] (1) Punching holes in the cellar: use a punching fork to punch holes in the bottom and wall of the cellar, and make small holes with a radius of 1.5cm and a depth of 4cm, 30 holes per square meter, and 50 holes near the upper part of the cellar. At -60cm, the eye-catching density becomes larger, with 50 small holes per square meter.

[0073] (2) after the hole is done, from the top of the cellar pool, pour the composite microbial bacterial agent obtained in the embodiment 1 into the hole, every pond uses the composite microbial bacterial agent 20kg, the long 3.4m of the cellar pond, wide 2.1m, 1.65m deep, cellar area 25m 2 .

[0074] (3) Curing: After adding pit mud into the cellar pool, smooth the holes, and limit the curing in summer before pressing the cellar. The temperature is set at 20-25°C. After one row of curing, the index of pit mud i...

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Abstract

The invention relates to a composite microbial agent and an application thereof in regulating and controlling the pH value of pit mud, and belongs to the technical field of biology and the technical field of fermentation. The invention provides a composite microbial agent. The composite microbial agent comprises composite microorganisms and yellow water; and the composite microorganisms comprise composite acetic acid utilization bacteria, comprise lactic acid utilization bacteria, comprise nitrate reducing bacteria, comprise actinomycetes and comprise sulfate reducing bacteria. The method disclosed by the invention follows the pit micro-ecological principle, and a variety of microorganisms such as the acetic acid utilization bacteria, the reducing bacteria, the actinomycetes and the lacticacid utilization bacteria are selected and combined for use to construct benign pit micro-ecology. The composite microbial agent provided by the invention can regulate the pH value of pit mud, so that the pH value of the pit mud is maintained at the optimal state of 6.0-6.8, aging of the pit mud is relieved, and the micro-ecological structure in the pit mud is stabilized.

Description

technical field [0001] The invention relates to a compound microbial bacterial agent and its application in regulating the pH of pit mud, and belongs to the field of biotechnology and fermentation technology. Background technique [0002] "A thousand-year-old cellar has been bad for thousands of years, and the quality of wine depends on the age of the cellar." High-quality cellar mud can significantly improve the quality of Luzhou-flavor liquor base wine. However, during the process of natural aging, a small number of pits will degenerate. Compared with degraded pit mud, the most intuitive manifestation is that the moisture and pH value of degraded pit mud are significantly lower than those of high-quality pit mud (between pH 4.1-5.6), the pit mud appears compacted and hardened, the surface of the pit mud is mixed with a large amount of calcium lactate and iron lactate crystals, and the metabolism of beneficial bacteria, caproic acid bacteria, butyric acid bacteria, and yeas...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12G3/02C12R1/085C12R1/01C12R1/125C12R1/145C12R1/47
CPCC12N1/20C12G3/02Y02W10/20
Inventor 谢旭苗秀珍刘乐乐吴琼张龙云朱超朱青
Owner JIANGSU YANGHE BREWERY JOINT STOCK
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