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Plant growth-promoting rhizobacteria strain Gxun-20 and application thereof in plant growth promotion

A technology of gxun-20, plant roots, applied in the directions of application, plant cultivation, seed and rhizome treatment, etc., can solve the problems of weak targeted growth promoting effect, unstable effect, and insufficient strain purity.

Active Publication Date: 2020-10-30
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the microbial fertilizers in my country have problems such as insufficient strain purity, weak targeted growth-promoting effect, and unstable effect.

Method used

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  • Plant growth-promoting rhizobacteria strain Gxun-20 and application thereof in plant growth promotion
  • Plant growth-promoting rhizobacteria strain Gxun-20 and application thereof in plant growth promotion
  • Plant growth-promoting rhizobacteria strain Gxun-20 and application thereof in plant growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Screening of plant rhizosphere growth-promoting strain Gxun-20

[0055] (1) Take 1g of mangrove root soil samples, add them to five shaker flasks filled with 20mL of sterile water, label them, put them in a shaker at 200r / min at 30°C for 30min, take them out and let them stand for 5~ 10min, obtain soil suspension;

[0056] (2) Take 5 mL of soil suspension from the five bottles of samples and enrich and culture them in LB liquid medium for 24 hours. The enriched bacterial liquid is diluted in gradient, and 100 μL of bacterial liquid is applied to the ADF solid medium for each gradient. cloth, each coated in three parallels, and then placed the coated plates in a 34°C constant temperature incubator for 3 days to obtain a single colony;

[0057] (3) Pick single colonies with different shapes and sizes on the plate and inoculate them on LB solid medium for separation and purification;

[0058] (4) Inoculate the isolated and purified strains into LB liquid medium contai...

Embodiment 2

[0083] Compared with Embodiment 1, the difference of this embodiment is:

[0084] The R 2 The preferred formula of A liquid culture medium is as follows:

[0085] Sucrose 0.4g, corn steep liquor 0.5g, casamino acid 0.5g, sodium chloride 0.1g, sodium pyruvate 0.3g, tryptone 0.5g, soluble starch 0.5g, L-tryptophan 2mg, magnesium sulfate heptahydrate 0.05g , dissolved in 1000mL water, with K 2 HPO 4 with KH 2 PO 4 Adjust the pH value to 6.5 and sterilize at 121°C for 20 min. The mass concentration of the sucrose is 0.4%; the mass concentration of the corn steep liquor is 0.05%; the mass concentration of the soluble starch is 0.05%.

[0086] The fermentation condition is shaking and fermenting at 28° C. for 38 hours in a shaker at 200 r / min.

[0087] The crop that present embodiment handles is wheat, and concrete method is as follows:

[0088] (1) Select plump, undamaged, and good-quality wheat seeds, soak them in warm water at 50°C for 10 minutes, pour them out, then soak...

Embodiment 3

[0094] Compared with Embodiment 1, the difference of this embodiment is:

[0095] The R 2 The preferred formula of A liquid culture medium is as follows:

[0096] Sucrose 0.4g, corn steep liquor 0.5g, casamino acid 0.5g, sodium chloride 0.1g, sodium pyruvate 0.3g, tryptone 0.5g, soluble starch 0.5g, L-tryptophan 2mg, magnesium sulfate heptahydrate 0.05g , dissolved in 1000mL water, with K 2 HPO 4 with KH 2 PO 4 Adjust the pH value to 6.2 and sterilize at 121°C for 20 min. The mass concentration of the sucrose is 0.4%; the mass concentration of the corn steep liquor is 0.05%; the mass concentration of the soluble starch is 0.05%.

[0097] The fermentation condition is shaking and fermenting at 28° C. for 38 hours in a shaker at 200 r / min.

[0098] The crop that present embodiment handles is capsicum, and concrete method is as follows:

[0099] (1) Select plump, undamaged, and good-quality chili seeds, soak them in warm water at 50°C for 10 minutes, then pour them out, t...

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Abstract

The invention provides a plant growth-promoting rhizobacteria strain Gxun-20 and application thereofin plant growth promotion. A bacterial strain with efficient IAA secretion, phosphorus dissolving and growth promoting capabilities is selected out through an ADF screening culture medium and an LB purification culture medium in a screening mode, 16S rRNA and physiological and biochemical characteristics of the bacterial strain are determined, the bacterial strain is determined to be brevibacillus brevis, the bacterial strain is gram-positive bacteria, and the bacterial colony morphology is round, smooth, convex and white. The strain is preserved in the Guangdong Microbial Culture Collection Center on June 13th, 2019. The fermentation condition of Gxun-20 is optimized, and the bacterial liquid obtained by fermentation is used for carrying out a growth-promoting test on plants. Compared with other strains, the strain Gxun-20 has higher IAA yield, and can be used for promoting the growth and development of plants, so that the use of chemical fertilizers and the damage of chemical fertilizers to soil are effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of plant growth promotion, and in particular relates to a plant rhizosphere growth promotion bacterial strain Gxun-20 and its application in plant growth promotion. Background technique [0002] According to research findings, excessive use of chemical fertilizers causes soil heavy metal pollution, reduces soil biological activity, soil pH imbalance, soil compaction, etc., which are not conducive to the healthy growth of crops. These effects lead to serious excessive heavy metal content in crops, and human consumption of food containing a large amount of heavy metals will threaten human health. In order to reduce the use of harmful chemical fertilizers and make human food healthy, it is necessary to introduce and research more healthy and effective chemical fertilizers. [0003] Plant rhizosphere growth-promoting bacteria are beneficial bacteria that can secrete surfactants, plant growth hormones such as 3-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01C1/00A01G22/00A01C21/00C12R1/01
CPCC12N1/20C12N1/02A01C1/00A01G22/00A01C21/005C12R2001/01C12N1/205
Inventor 姜明国申乃坤杨立芳王一兵丰景许剑
Owner GUANGXI UNIV FOR NATITIES
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