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Application of prawnella in treatment or prevention of osteoporosis

A technology for Prevotella and osteoporosis, applied in the field of microbial applications, can solve the problems of bisphosphonate side effects that cannot be ignored, dependence on malignant tumors, and the inability to meet the conditions for long-term maintenance of osteoporosis.

Inactive Publication Date: 2020-10-30
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many types of anti-osteoporosis drugs, long-term use of these drugs has side effects such as gastrointestinal reactions, nephrotoxicity, atypical femur fractures, mandibular osteonecrosis, and estrogen-dependent malignant tumors, and is expensive
Osteoporosis is a disease that requires long-term continuous treatment. Except for bisphosphonates, the curative effect of other drugs declines rapidly after drug withdrawal. However, the side effects caused by long-term use of bisphosphonates cannot be ignored. Therefore, the current guidelines It is recommended that the course of oral therapy should not exceed 5 years, but this does not meet the long-term maintenance conditions required for the treatment of postmenopausal osteoporosis

Method used

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  • Application of prawnella in treatment or prevention of osteoporosis
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  • Application of prawnella in treatment or prevention of osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] Preparation of animal models

[0030] Mouse bilateral ovariectomy model: after intraperitoneal injection of 3.6% chloral hydrate (10ml / kg) for anesthesia, incise the back skin and muscle layer layer by layer, enter the abdominal cavity, find out the bilateral ovaries, and ligate the fallopian tubes. The ovaries were completely removed and sutured in layers. All mice were intramuscularly injected with penicillin 50,000 U / d for 3 days to prevent infection. After 1 week of postoperative recovery, the experiment was carried out in groups.

[0031] Mouse sham operation model: after intraperitoneal injection of 3.6% chloral hydrate (10ml / kg) for anesthesia, cut the back skin and muscular layer layer by layer, enter the abdominal cavity, find out the bilateral ovaries, and remove only the part of the abdomen around the ovaries After the fat was sutured layer by layer, all mice were intramuscularly injected with penicillin 50,000 U / d for 3 days to prevent infection. After 1 ...

Embodiment 1

[0040] 1. Example 1: P. histicola improves osteoporosis caused by estrogen deficiency in mice.

[0041] Micro-CT scanning and analysis

[0042] After the mice were sacrificed, the mouse femurs were dissected, and the soft tissues attached to the bones were removed, and then fixed in 4% tissue fixative solution for 24 hours. After the fixation, the right femur was scanned with Skyscan 1176 Micro-CT instrument (Bruker microCT, Kontich, Belgium), and the parameters were set as follows: source voltage, 50kV; source current, 500μA; AI 0.5mm filter; pixel size 9μm; rotation step, 0.4 degree. The scanned images were reconstructed using NReconru software (Bruker microCT, Kontich, Belgium), with the following parameter settings: ring artefact correction, 7; smoothing, 2; beam hardening correction, 40%. A more precise analysis area was defined as 0.5 mm above the growth plate, and the analysis thickness was 1 mm. The trabecular bone region of interest (region of interest, ROI) in this...

Embodiment 2

[0044] 2. Example 2: P.histicola bacteria can inhibit the increase in osteoclast activity caused by estrogen deficiency and improve the systemic inflammatory response and bone resorption level

[0045] Decalcification, paraffin embedding and sectioning

[0046] After Micro-CT analysis, femurs were decalcified by immersion in 14% EDTA (Sigma-Aldrich, Sydney, NSW, Australia) solution at 37°C for 7 days, and the solution was changed every two days. After decalcification was completed, paraffin embedding was carried out. The embedding scheme is shown in Table 2 below:

[0047] Femoral Paraffin Embedding Protocol

[0048]

[0049]

[0050] Table 2

[0051] After embedding, the specimens were put into a square mold, poured with liquid paraffin, and sliced ​​on a paraffin microtome after cold cutting with a thickness of 6 μm.

[0052] Dewaxing and Dyeing

[0053] Dewax the cut paraffin sections according to the following table 3:

[0054] Section Dewaxing Protocol

[0055...

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Abstract

The invention relates to an application of prawnella in treatment or prevention of osteoporosis. The P.histicola bacteria are used as symbiotic bacteria in normal human intestinal tracts and have excellent safety, animal experiments on estrogen-deficient mice find that the P.histacola can be used for preventing bone mass loss of the mice; the imbalance of intestinal flora caused by estrogen deficiency is regulated, the expression of inflammatory factors is inhibited, the intestinal permeability increase caused by estrogen deficiency is reduced, then inflammatory factors in the intestinal tractwall are reduced, differentiation, maturation and activation of osteoclasts guided by RANKL are inhibited; the P.histacola bacteria have effects of osteoporosis resistance, bone absorption reducing,bone mass loss inhibition and estrogen deficiency osteoporosis resistance, and can meet the requirements of long-term taking, economy, safety and no side effect required by osteoporosis prevention andtreatment as the micro-ecological preparation.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to the application of a Prevotella genus in the treatment or prevention of osteoporosis. Background technique [0002] Osteoporosis (osteoporosis OP) is a systemic bone disease characterized by decreased bone mass, destruction of bone tissue microstructure, increased bone fragility, and susceptibility to fracture. It is a common senile disease. Osteoporotic fractures are very harmful and are one of the main causes of disability and death in elderly patients. Within 1 year after hip fracture, 20% of patients will die from various complications, about 50% of patients will be disabled, and the quality of life will be significantly reduced. Moreover, the medical treatment and nursing of osteoporosis and fractures need to invest a lot of manpower, material and financial resources, causing heavy family and social burdens. It is predicted that in 2015, 2035 and 2050, the me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P19/10
CPCA61K35/74A61P19/10
Inventor 陈雷金可可王中祥
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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